公开(公告)号：US06211988B1

公开(公告)日：2001-04-03

申请号：US09331457

申请日：1999-08-25

Applicant: Johann Engelhardt ,  Heinrich Ulrich

Inventor： Johann Engelhardt ,  Heinrich Ulrich

IPC: G02B2608

CPC classification number: G02B21/0048 ,  G02B21/0024 ,  G02B21/0072 ,  G02B21/0084

Abstract: The invention relates to an optical device for scanning a beam in two axes that are substantially perpendicular to each other, for use in particular in confocal laser scan microscopes, and aims to avoid serious image defects. The invention is characterized in that it has three mirrors (1, 2; 3) of which two mirrors (1, 2) are fixedly positioned at an angle to each other so that they rotate together around the y-axis and in so doing rotate the beam (4) around a pivot point located on the axis of rotation (x-axis) of the third mirror (3) which rotates by itself.

Abstract translation: 本发明涉及一种用于扫描基本上彼此垂直的两个轴的光束的光学装置，特别是用于共焦激光扫描显微镜中，并且旨在避免严重的图像缺陷。 本发明的特征在于其具有三个反射镜（1,2; 3），其中两个反射镜（1,2）以彼此成一定角度固定地定位，使得它们围绕y轴一起旋转，并且旋转 所述梁（4）围绕位于所述第三反射镜（3）的旋转轴线（x轴）上的枢转点自身旋转。

公开(公告)号：US6002509A

公开(公告)日：1999-12-14

申请号：US817618

申请日：1997-04-17

Applicant: Roelof W. Wijnaendts Van Resandt ,  Joachim Jehle ,  Johann Engelhardt

Inventor： Roelof W. Wijnaendts Van Resandt ,  Joachim Jehle ,  Johann Engelhardt

IPC: G02B21/00 ,  G02B26/02

CPC classification number: G02B21/0044

Abstract: A beam scanner is disclosed for confocal microscopes. The beam scanner is provided with a disc-shaped, rotary-driven base unit which largely prevents disruptive optical scattering. The beam scanner is so designed that at least one main surface of the base unit is provided with a reflecting grid; light is reflected only by those regions of the main surface which correspond to the meshes of the reflecting grid, or only by those regions which correspond to the lattice of the reflecting grid.

Abstract translation: PCT No.PCT / US95 / 01463 Sec。 371日期：1998年4月17日 102（e）日期1998年4月17日PCT 1995年10月21日PCT PCT。 公开号WO96 / 12982 日期：1996年5月2日公开了一种用于共聚焦显微镜的光束扫描器。 光束扫描器设置有盘状的旋转驱动基座单元，其大大防止破坏性的光散射。 光束扫描器被设计成使得基座单元的至少一个主表面设置有反射栅格; 光仅由对应于反射栅格的网格的主表面的那些区域反射，或者仅由对应于反射栅格的晶格的那些区域反射。

公开(公告)号：US20120104279A1

公开(公告)日：2012-05-03

申请号：US13297872

申请日：2011-11-16

Applicant: Matthias Reuss ,  Johann Engelhardt

Inventor： Matthias Reuss ,  Johann Engelhardt

IPC: G01N21/64

CPC classification number: G02B21/0032 ,  G02B21/0068 ,  G02B21/0076

Abstract: A fluorescence light scanning microscope (2) comprises a light source providing excitation light (8) for exciting a fluorophore in a sample to be imaged for spontaneous emission of fluorescence light, and suppression light (7) for suppressing spontaneous emission of fluorescence light by the fluorophore on a common optical axis (4), the suppression wavelength differing from the excitation wavelength; an objective (19) focusing both the excitation (8) and the suppression (7) light to a focus point; a detector (21) detecting fluorescence light (11) spontaneously emitted by the fluorophore; and a chromatic beam shaping device (1) arranged on the common optical axis (4), and including a birefringent chromatic optical element (3) adapted to shape a polarization distribution of the suppression light (7) such as to produce an intensity zero at the focus point, and to leave the excitation light such as to produce a maximum at the focus point.

Abstract translation: 荧光光扫描显微镜（2）包括提供用于激发要成像的样品中的荧光团以激发荧光的自发发射的激发光（8）的光源，以及用于抑制荧光的自发发射的抑制光（7） 荧光团在共同的光轴（4）上，抑制波长与激发波长不同; 将激发（8）和抑制（7）光聚焦到焦点的目标（19） 检测器（21），检测由荧光团自发发射的荧光（11）; 以及布置在公共光轴（4）上的色光束整形装置（1），并且包括适于使抑制光（7）的偏振分布成形的双折射彩色光学元件（3），以产生在 焦点，并留下激发光，以便在焦点处产生最大值。

公开(公告)号：US07897937B2

公开(公告)日：2011-03-01

申请号：US12413665

申请日：2009-03-30

Applicant: Johann Engelhardt ,  Marion Lang ,  Stefan Hell

Inventor： Johann Engelhardt ,  Marion Lang ,  Stefan Hell

IPC: G01N21/64

CPC classification number: G01N21/6458 ,  G01N21/6428

Abstract: For imaging a structure in a sample with spatial resolution, the structure is labeled with a fluorophore which is transferable by an optical transfer signal out of a first into a second photochromic state. Via a common objective, the sample is subjected to both the focussed optical transfer signal and a focussed optical excitation signal only exciting a portion of the fluorophore being in its second photochromic state for fluorescence. The transfer and the excitation signal have a common centre of maximum intensity; and a decrease of intensity of the transfer signal with the distance to this common centre is substantially stronger than any decrease of the effective return rate of the fluorophore back into the first photochromic state. Fluorescence light emitted by the excited fluorophore is detected. Then, the common centre is shifted with regard to the sample; and the steps of subjecting and detecting are repeated.

Abstract translation: 为了对具有空间分辨率的样品中的结构进行成像，结构用荧光团标记，荧光团可通过第一光致变色状态的光转移信号转移。 通过共同的目标，样品经受聚焦的光学转移信号和聚焦的光学激发信号，仅激发处于其第二光致变色状态的荧光团的一部分用于荧光。 传输和激励信号具有共同的最大强度中心; 并且与该公共中心的距离的传输信号的强度的降低比荧光团回到第一光致变色状态的有效返回速率的任何降低显着更强。 检测由激发的荧光团发射的荧光。 然后，公共中心相对于样本移动; 重复进行检测的步骤。

公开(公告)号：US07746470B2

公开(公告)日：2010-06-29

申请号：US11910938

申请日：2006-03-31

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G01J4/00

CPC classification number: G02B21/0076 ,  G02B21/0036

Abstract: The present invention relates to an optical scanning device that comprises a light source to emit a beam of light, and a beam splitter to split that beam into several beamlets, and further a first objective lens to direct said beamlets onto a focal plane wherein each of said beamlets impinges on the focal plane spacially separated from each other, wherein the beam splitter comprises several birefringent elements for splitting said beam, preferably a stack of Wollaston prisms.

Abstract translation: 本发明涉及一种包括发射光束的光源的光学扫描装置，以及将该光束分成几个子束的分束器，以及另外的第一物镜，以将所述子束引导到焦平面上，其中 所述子束照射在彼此空间上分离的焦平面上，其中分束器包括若干双折射元件，用于分裂所述光束，优选地是Wollaston棱镜的堆叠。

公开(公告)号：US06975394B2

公开(公告)日：2005-12-13

申请号：US09987364

申请日：2001-11-14

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G01N21/64 ,  G02B21/16

CPC classification number: G01N21/6458 ,  B82Y15/00 ,  G01N21/6408 ,  G01N21/6428 ,  G02B21/16

Abstract: An apparatus for measuring the lifetime of an excited state in a specimen is disclosed. The apparatus comprises an electromagnetic energy source (1) that emits light (3) of one wavelength. Also provided are a means (5) for dividing the light (3) into at least a first and a second partial light beam (7, 9) and an intermediate element (23) in at least one partial light beam to influence the transit time.

Abstract translation: 公开了一种用于测量试样中的激发态的寿命的装置。 该装置包括发射一个波长的光（3）的电磁能源（1）。 还提供了一种用于将光（3）分成至少一个部分光束中的第一和第二部分光束（7,9）和中间元件（23）的装置（5），以影响传播时间 。

公开(公告)号：US06961124B2

公开(公告)日：2005-11-01

申请号：US10133654

申请日：2002-04-26

Applicant: Johann Engelhardt ,  Juergen Hoffmann

Inventor： Johann Engelhardt ,  Juergen Hoffmann

IPC: G01J3/36 ,  G01N21/64 ,  G02B21/00 ,  G02B21/06 ,  G01J3/30

CPC classification number: G01N21/6458 ,  G01J3/36 ,  G01N2021/6441 ,  G02B21/0048 ,  G02B21/0064 ,  G02B21/0076 ,  G02B2207/114

Abstract: A method for examining a specimen (27) that exhibits at least two optical transition lines and is optically excitable at least with light of a first and light of a second wavelength is characterized by the step of illuminating the specimen (27) with illuminating light (15) that generates at least a multiple of the first wavelength and a multiple of the second wavelength; and by the step of detecting the detected light (29) proceeding from the specimen (27).Also disclosed is a scanning microscope system (1) having at least one light source (3) that emits illuminating light (15) for illumination of a specimen (27), the specimen (27) exhibiting at least two optical transition lines and being optically excitable at least with light of a first and light of a second wavelength, having at least one detector (41, 43, 65, 77, 79) for detection of the detected light (29) proceeding from the specimen (27) and an objective (25) for focusing the illuminating light (15) onto a subregion of the specimen (27). The scanning microscope system is characterized in that the illuminating light (15) generates at least a multiple of the first wavelength and a multiple of the second wavelength.

Abstract translation: 用于检查至少具有第一和第二波长的光的至少两个光学过渡线并且是光学上可激发的检测器（27）的方法的特征在于用照明光照射样品（27）的步骤 15），其产生所述第一波长的至少一倍和所述第二波长的倍数; 并且通过检测从样本（27）进行的检测光（29）的步骤。 还公开了一种扫描显微镜系统（1），其具有发射用于照射样本（27）的照明光（15）的至少一个光源（3），所述检体（27）显示出至少两个光学过渡线并且是光学的 至少具有第一和第二波长的光的可兴奋的，具有用于检测从样本（27）进行的检测光（29）的至少一个检测器（41,43,65,77,79）和一个目标 （25），用于将照明光（15）聚焦到样本（27）的子区域上。 扫描显微镜系统的特征在于，照明光（15）产生第一波长的倍数和第二波长的倍数。

公开(公告)号：US06934022B1

公开(公告)日：2005-08-23

申请号：US09857960

申请日：1999-12-10

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G01N33/483 ,  G01N21/27 ,  G01N21/39 ,  G01N21/49 ,  G01N21/55 ,  G02B21/00 ,  G02B21/06 ,  G02B21/36 ,  G01N15/02 ,  G01N21/00

CPC classification number: G01N21/6458 ,  G01N21/554 ,  G01N21/648 ,  G02B21/0068 ,  G02B21/0072 ,  G02B21/0076

Abstract: The invention relates to a method for examining different structures in preferably biological preparations in a differential manner, especially by means of confocal laser scanning microscopy. The method is characterized in that particles having a specific diameter and specific characteristics are assigned to the structures and in that said structures are detected by detecting the particles which have specifically bonded in or to the preparations. The detection process is carried out in an advantageous manner by marking the structures with metal particles with diameters of 10 nm to 1,500 nm and detecting Mie scattering or a plasmon signal.

Abstract translation: 本发明涉及以差别方式，特别是通过共焦激光扫描显微镜检查优选生物制剂中的不同结构的方法。 该方法的特征在于具有特定直径和特定特性的颗粒被分配给结构，并且通过检测特异性结合到制剂中的颗粒来检测所述结构。 通过用直径为10nm〜1500nm的金属颗粒标记结构并检测米氏散射或等离子体信号，以有利的方式进行检测处理。

公开(公告)号：US06888118B2

公开(公告)日：2005-05-03

申请号：US09878842

申请日：2001-06-11

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G02B7/00 ,  G02B21/00 ,  G02F1/03 ,  G02F1/11 ,  G02F1/33 ,  G05D23/19 ,  G02B7/04

CPC classification number: G02B21/0064 ,  G02B7/008 ,  G02B21/0032 ,  G02B21/0052 ,  G02F1/113 ,  G02F1/116 ,  G05D23/1919

Abstract: The present invention concerns a method and an apparatus for stabilizing the temperature of optical, in particular optically active, electrooptical, or acoustooptical components, preferably in scanning microscopy, in particular in confocal scanning microscopy, such that the temperature of the component can be held in stable fashion at a constant value in a space-saving manner, as simply as possible, and with as few additional assemblies as possible, and is characterized in that the energy that interacts with the component serves for stabilization.

Abstract translation: 本发明涉及一种用于稳定光学特别是光学活性的电光学或声光组件的温度的方法和装置，优选地在扫描显微术中，特别是在共聚焦扫描显微术中，使得组件的温度可以保持在 以尽可能简单的节省空间的方式以恒定的值稳定地进行，并且尽可能少的附加组件，其特征在于与组件相互作用的能量用于稳定化。

公开(公告)号：US06864989B2

公开(公告)日：2005-03-08

申请号：US09939726

申请日：2001-08-28

Applicant: Rafael Storz ,  Johann Engelhardt ,  Holger Birk

Inventor： Rafael Storz ,  Johann Engelhardt ,  Holger Birk

IPC: G02B26/06 ,  G02B21/00 ,  G02B21/06 ,  G02F1/01 ,  H01S3/00 ,  G01B11/24

CPC classification number: G02B21/0024 ,  G02B21/0056

Abstract: A method for illuminating an object with light (2) from a laser light source (3), preferably in a confocal scanning microscope (1). With the method according to the invention, it is possible to reduce the coherence length of the laser light, so that disruptive interference phenomena can be substantially eliminated. Should interference phenomena nevertheless be formed, these are to be influenced in such a way that they have no effect on the detection. The method according to the invention is characterized in that the phase angle of the light field is varied by a modulator in such a way that interference phenomena do not occur in the optical beam path, or occur only to an undetectable extent, within a predeterminable time interval.

Abstract translation: 优选在共焦扫描显微镜（1）中从激光光源（3）用光（2）照射物体的方法。 利用根据本发明的方法，可以减少激光的相干长度，从而可以基本上消除破坏性干扰现象。 应该如何形成干扰现象，这些影响将不会对检测产生影响。 根据本发明的方法的特征在于，光场的相位角由调制器改变，使得在预定时间内在光束路径中不发生干涉现象或仅发生不可检测的程度 间隔。