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    Methods and apparatus for measuring analytes using large scale FET arrays
    32.
    发明授权
    Methods and apparatus for measuring analytes using large scale FET arrays 有权

    公开(公告)号：US11530444B2

    公开(公告)日：2022-12-20

    申请号：US16882414

    申请日：2020-05-22

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

    IPC: C12Q1/6874 ,  G01N27/414 ,  B01L3/00 ,  C12Q1/6869

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

    Method for measuring analytes using large scale chemfet arrays
    33.
    发明授权
    Method for measuring analytes using large scale chemfet arrays 有权

    公开(公告)号：US10816506B2

    公开(公告)日：2020-10-27

    申请号：US16677666

    申请日：2019-11-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

    IPC: G01N27/414 ,  C12Q1/6874 ,  H01L29/78 ,  H01L27/088 ,  C12Q1/6818 ,  G01N33/543 ,  H01L29/423

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

    Nucleic acid amplification
    36.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US10329544B2

    公开(公告)日：2019-06-25

    申请号：US15091717

    申请日：2016-04-06

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao ,  Wolfgang Hinz

    IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

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