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    Microfluidic apparatus, method, and applications
    31.
    发明授权
    Microfluidic apparatus, method, and applications 有权

    公开(公告)号：US10081828B2

    公开(公告)日：2018-09-25

    申请号：US14478049

    申请日：2014-09-05

    Applicant: RHEONIX, INC.

    Inventor： Peng Zhou

    IPC: C12Q1/68 ,  C12Q1/6806 ,  C07H1/08 ,  B01L3/00 ,  C12N15/10

    CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L2200/0631 ,  C07H1/08 ,  C12N15/1003 ,  C12Q2527/101 ,  C12Q2565/629

    Abstract: A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.

    Microfluidic device-based nucleic acid purification method
    34.
    发明授权
    Microfluidic device-based nucleic acid purification method 有权
    基于微流控装置的核酸纯化方法

    公开(公告)号：US09169479B2

    公开(公告)日：2015-10-27

    申请号：US14226204

    申请日：2014-03-26

    Applicant: RHEONIX, INC.

    Inventor： Lincoln C. Young ,  Peng Zhou ,  Gwendolyn Spizz ,  Rubina Yasmin

    IPC: C12Q1/68 ,  C12N15/10

    CPC classification number: C12N15/1013 ,  C12Q1/6806

    Abstract: A method is provided for purifying nucleic acid from a sample in a microfluidic device. The method can be used to purify nucleic acids from any source known in the art that comprises nucleic acids, such as prokaryotic or eukaryotic organisms, viruses, cell, tissues, organs, etc. In a specific example, the tissue is whole blood. The method for purifying nucleic acid may run fully automated in the microfluidic device.

    Abstract translation: 提供了一种用于从微流体装置中的样品中纯化核酸的方法。 该方法可用于从本领域已知的任何来源纯化核酸，其包含核酸，例如原核或真核生物，病毒，细胞，组织，器官等。在具体实例中，组织是全血。 纯化核酸的方法可以在微流体装置中完全自动化。

    CHANNEL-LESS PUMP, METHODS, AND APPLICATIONS THEREOF
    36.
    发明申请
    CHANNEL-LESS PUMP, METHODS, AND APPLICATIONS THEREOF 审中-公开
    无通道泵，其方法和应用

    公开(公告)号：US20150147195A1

    公开(公告)日：2015-05-28

    申请号：US14548474

    申请日：2014-11-20

    Applicant: Rheonix, Inc.

    Inventor： Lincoln C. Young ,  Peng Zhou

    IPC: F04B43/14 ,  F04B43/04 ,  F04B43/06

    CPC classification number: F04B43/043 ,  B01L3/50273 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1827 ,  B01L2400/0481 ,  F04B43/0054 ,  F04B43/02 ,  F04B43/06 ,  F04B43/12

    Abstract: A channel-less microfluidic pump includes a cartridge including a substrate and an actuatable film layer disposed on the substrate, and a manifold having at least three actuatable void volumes separated by a plurality of wall sections and an actuatable flexible layer disposed on the manifold interfacing the actuatable film layer. In operation, the pump can be in an unactuated state wherein the actuatable film layer is disposed against the surface of the substrate or an actuated state wherein at least a portion of the flexible layer and a corresponding portion of the actuatable film layer are deflected into a corresponding void volume thus forming a fluidic volume between the deflected portion of the actuatable film layer and the surface of the substrate. In the actuated state, there is a fluidic gap between immediately adjacent void volumes formed by a thinned region of the flexible layer at a point of contact with a top surface of a wall section. A method of transporting fluid using the channel-less microfluidic pump is described.

    Abstract translation: 无通道微流体泵包括一个包括一个基板和一个设置在该基板上的致动膜层的盒，以及一个歧管，具有由多个壁部分分开的至少三个可致动的空隙体积，以及设置在歧管上的可致动的柔性层， 可致动膜层。 在操作中，泵可以处于未致动状态，其中可致动膜层设置在基板的表面上或致动状态，其中柔性层的至少一部分和可致动膜层的相应部分偏转成 从而在可致动膜层的偏转部分和基底表面之间形成流体体积。 在致动状态下，在与壁部分的顶表面的接触点处的柔性层的薄化区域形成的紧邻的空隙体之间存在流体间隙。 描述了使用无通道微流体泵输送流体的方法。

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