公开(公告)号：US20130079248A1

公开(公告)日：2013-03-28

申请号：US13584552

申请日：2012-08-13

Applicant: Minseok S. KIM ,  Sun-soo KIM ,  Jin-hoon KIM ,  Won-ho LEE ,  Jeong-gun LEE ,  Tae-seok SIM ,  Sang-hyun BAEK ,  Hyo-young JEONG

Inventor： Minseok S. KIM ,  Sun-soo KIM ,  Jin-hoon KIM ,  Won-ho LEE ,  Jeong-gun LEE ,  Tae-seok SIM ,  Sang-hyun BAEK ,  Hyo-young JEONG

IPC: C12M1/00 ,  C40B40/12 ,  C40B40/06 ,  C40B40/10 ,  F16L55/027 ,  B01D35/00

CPC classification number: B01L3/502753 ,  B01L3/502746 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0487 ,  B01L2400/086 ,  G01N21/05 ,  G01N2001/4088 ,  G01N2021/0325 ,  G01N2021/0346

Abstract: A fluid controlling apparatus including an inlet through which a fluid is introduced, a channel portion connected to the inlet, an outlet that is connected to the channel portion and through which the fluid is discharged, and at least one fluid resisting portion disposed between the inlet and the outlet, as well as a filter and a biochip including the fluid controlling apparatus.

Abstract translation: 一种流体控制装置，其包括入口，流体通过该入口，与入口连接的通道部分，连接到通道部分并且流体被排出的出口以及设置在入口之间的至少一个流体阻力部分 和出口，以及包括流体控制装置的过滤器和生物芯片。

公开(公告)号：US20120322132A1

公开(公告)日：2012-12-20

申请号：US13594293

申请日：2012-08-24

Applicant: Yoon-kyoung CHO ,  Jeong-gun LEE ,  Beom-seok LEE ,  Jong-myeon PARK

Inventor： Yoon-kyoung CHO ,  Jeong-gun LEE ,  Beom-seok LEE ,  Jong-myeon PARK

IPC: C12M1/10 ,  G01N1/34 ,  C12N13/00

CPC classification number: B01L3/502738 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/0233 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/004 ,  F16K2099/0076 ,  F16K2099/0084

Abstract: A centrifugal force-based microfluidic device for nucleic acid extraction and a microfluidic system are provided. The microfluidic device includes a body of revolution; a microfluidic structure disposed in the body of revolution, the microfluidic structure including a plurality of chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structure transmitting the fluid using centrifugal force due to rotation of the body of revolution; and magnetic beads contained in one of the chambers which collect a target material from a biomaterial sample flowing into the chamber, wherein the microfluidic structure washes the magnetic beads which collect the target material, and separates nucleic acid by electromagnetic wave irradiation from an external energy source to the magnetic beads. The microfluidic system includes the microfluidic device; a rotation operating unit which rotates the body of revolution; and an external energy source which irradiates electromagnetic waves.

Abstract translation: 提供了用于核酸提取的离心力微流体装置和微流体系统。 微流体装置包括一个革命体; 设置在旋转体内的微流体结构，所述微流体结构包括多个室，连接所述室的通道和设置在通道中的阀以控制流体流动，所述微流体结构由于所述体的旋转而使用离心力传递流体 的革命 以及包含在其中一个室中的磁珠，其从流入腔室的生物材料样品收集目标材料，其中微流体结构洗涤收集目标材料的磁珠，并通过来自外部能量源的电磁波照射分离核酸 到磁珠。 微流体系统包括微流体装置; 使旋转体旋转的旋转操作单元; 以及照射电磁波的外部能量源。

公开(公告)号：US20120028850A1

公开(公告)日：2012-02-02

申请号：US13269249

申请日：2011-10-07

Applicant: Beom-seok LEE ,  Yoon-kyoung CHO ,  Jong-myeon PARK ,  Jeong-gun LEE

Inventor： Beom-seok LEE ,  Yoon-kyoung CHO ,  Jong-myeon PARK ,  Jeong-gun LEE

IPC: C40B60/12 ,  G01N33/48

CPC classification number: B01L3/502738 ,  B01L3/502723 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0677 ,  B01L2400/084 ,  B01L2400/086 ,  F16K99/0001 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/004 ,  F16K99/0061 ,  F16K2099/0084 ,  G01N35/00069

Abstract: Provided is a rotatable microfluidic device for conducting simultaneously two or more assays. The device includes a platform which can be rotated, a first unit which is disposed at one portion of the platform and detects a target material from a sample using surface on which a capture probe selectively binds to the target material is attached, and a second unit which is disposed at another portion of the platform and detects a target material included in the sample by a different reaction from the reaction conducted in the first unit.

公开(公告)号：US20110180251A1

公开(公告)日：2011-07-28

申请号：US13081193

申请日：2011-04-06

Applicant: Jeong-gun LEE ,  Yong-moo Shin ,  Kui-hyun Kim ,  Jin-han Chung

Inventor： Jeong-gun LEE ,  Yong-moo Shin ,  Kui-hyun Kim ,  Jin-han Chung

IPC: F25B29/00 ,  G05D23/00

CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L9/527 ,  B01L2300/0681 ,  B01L2300/0806 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2300/1883 ,  B01L2400/0409 ,  G01N35/00069 ,  G01N2035/00346 ,  Y10T436/11 ,  Y10T436/25

Abstract: Provided is a biochemical analyzer including: a microfluidic device loading space including a microfluidic device supporting unit detachably supporting a microfluidic device including an electromagnetic radiation application region in which electromagnetic energy is applied; an energy source loading space including an energy source applying the electromagnetic energy to the electromagnetic radiation application region; and an isolation wall isolating the microfluidic device loading space and the energy source loading space to prevent heat transfer between the microfluidic device loading space and the energy source loading space and including a transparent window through which the electromagnetic energy can be transmitted. A method of controlling an internal temperature of the biochemical analyzer is also provided.

Abstract translation: 提供了一种生物化学分析仪，其包括：微流体装置装载空间，包括微流体装置支撑单元，其可拆卸地支撑包括施加电磁能的电磁辐射施加区域的微流体装置; 能量源装载空间，其包括将电磁能施加到电磁辐射施加区域的能量源; 以及隔离壁，隔离微流体装置加载空间和能量源装载空间，以防止微流体装置加载空间和能量源装载空间之间的热传递，并且包括可透射电磁能的透明窗口。 还提供了一种控制生化分析仪内部温度的方法。

公开(公告)号：US20110143414A1

公开(公告)日：2011-06-16

申请号：US13034857

申请日：2011-02-25

Applicant: Yoon-kyoung CHO ,  Jeong-gun LEE ,  Sung-young JEONG

Inventor： Yoon-kyoung CHO ,  Jeong-gun LEE ,  Sung-young JEONG

IPC: C12N13/00

CPC classification number: B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/1861 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/043 ,  B01L2400/0439 ,  C12M47/02 ,  C12M47/04 ,  C12M47/06 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A microfluidic device for the concentration and lysis of cells or viruses and a method of concentrating and lysing cells or viruses using the microfluidic device include: magnetic beads, a reaction chamber in which the magnetic beads are accommodated and a laser source. The reaction chamber includes a plurality of electrodes which cross each other and are separated by a dielectric to generate an electric field and a vibrating part agitating the magnetic beads in the chamber. The laser source radiates a laser onto the magnetic beads in the reaction chamber.

Abstract translation: 用于细胞或病毒的浓缩和裂解的微流体装置以及使用微流体装置浓缩和裂解细胞或病毒的方法包括：磁珠，容纳磁珠的反应室和激光源。 反应室包括彼此交叉并被电介质隔开以产生电场的多个电极以及搅拌室中的磁珠的振动部分。 激光源将激光辐射到反应室中的磁珠上。

公开(公告)号：US20110126918A1

公开(公告)日：2011-06-02

申请号：US12979049

申请日：2010-12-27

Applicant: Jong-myeon PARK ,  Yoon-kyuong CHO ,  Jeong-gun LEE ,  Beom-seok LEE ,  Sung-woo HONG

Inventor： Jong-myeon PARK ,  Yoon-kyuong CHO ,  Jeong-gun LEE ,  Beom-seok LEE ,  Sung-woo HONG

IPC: F16K49/00

CPC classification number: F16K99/0019 ,  B01L3/502738 ,  B01L2300/0803 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0032 ,  F16K99/0034 ,  F16K99/004 ,  F16K2099/0084 ,  Y10T137/1827 ,  Y10T137/2196 ,  Y10T137/6606 ,  Y10T137/7755

Abstract: Provided are a valve unit and a reaction apparatus having the valve unit. The valve unit includes a phase transition material, which melts and expands upon an application of the electromagnetic waves to the valve filler, and the valve filler is directed into the channel through the connection passage and closes the channel. The valve unit also includes heat generation particles, which are dispersed in the phase transition material and generate heat upon an application of electromagnetic wave energy.

Abstract translation: 提供具有阀单元的阀单元和反应装置。 阀单元包括相变材料，其在将电磁波施加到阀填充器时熔化和膨胀，并且阀填料通过连接通道引导到通道中并关闭通道。 阀单元还包括发散颗粒，其分散在相变材料中并且在施加电磁波能量时产生热量。

公开(公告)号：US20090181412A1

公开(公告)日：2009-07-16

申请号：US12332446

申请日：2008-12-11

Applicant: Yoon-kyoung CHO ,  Hee-Kyun LIM ,  Jeong-gun LEE ,  Ki-eun KIM ,  Sung-ho LEE ,  Chae-joon LEE

Inventor： Yoon-kyoung CHO ,  Hee-Kyun LIM ,  Jeong-gun LEE ,  Ki-eun KIM ,  Sung-ho LEE ,  Chae-joon LEE

IPC: C12Q1/62 ,  C12Q1/48 ,  C12Q1/32 ,  C12Q1/42 ,  G01N33/92 ,  G01N33/00 ,  C12Q1/60 ,  C12Q1/58 ,  C12Q1/54

CPC classification number: B01L3/50273 ,  B01L2200/16 ,  B01L2300/0803 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0409

Abstract: Provided is a method of storing a reagent in a microfluidic device. The reagent, in a liquid form, is loaded into reaction chambers arranged on the microfluidic device; followed by lyophilization as being contained in the microfluidic device.

Abstract translation: 提供了将试剂储存在微流体装置中的方法。 将液体形式的试剂装载到布置在微流体装置上的反应室中; 随后冻干包被在微流体装置中。

公开(公告)号：US20080108120A1

公开(公告)日：2008-05-08

申请号：US11848748

申请日：2007-08-31

Applicant: Yoon-kyoung CHO ,  Jeong-gun LEE ,  Beom-seok LEE ,  Jong-myeon PARK

Inventor： Yoon-kyoung CHO ,  Jeong-gun LEE ,  Beom-seok LEE ,  Jong-myeon PARK

IPC: C12N13/00 ,  B04B5/10 ,  C12M1/10

CPC classification number: B01L3/502738 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/0233 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/004 ,  F16K2099/0076 ,  F16K2099/0084

Abstract: A centrifugal force-based microfluidic device for nucleic acid extraction and a microfluidic system are provided. The microfluidic device includes a body of revolution; a microfluidic structure disposed in the body of revolution, the microfluidic structure including a plurality of chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structure transmitting the fluid using centrifugal force due to rotation of the body of revolution; and magnetic beads contained in one of the chambers which collect a target material from a biomaterial sample flowing into the chamber, wherein the microfluidic structure washes the magnetic beads which collect the target material, and separates nucleic acid by electromagnetic wave irradiation from an external energy source to the magnetic beads. The microfluidic system includes the microfluidic device; a rotation operating unit which rotates the body of revolution; and an external energy source which irradiates electromagnetic waves.

Abstract translation: 提供了用于核酸提取的离心力微流体装置和微流体系统。 微流体装置包括一个革命体; 设置在旋转体内的微流体结构，所述微流体结构包括多个室，连接所述室的通道和设置在通道中的阀以控制流体流动，所述微流体结构由于所述体的旋转而使用离心力传递流体 的革命 以及包含在其中一个室中的磁珠，其从流入腔室的生物材料样品收集目标材料，其中微流体结构洗涤收集目标材料的磁珠，并通过来自外部能量源的电磁波照射分离核酸 到磁珠。 微流体系统包括微流体装置; 使旋转体旋转的旋转操作单元; 以及照射电磁波的外部能量源。

公开(公告)号：US20070231216A1

公开(公告)日：2007-10-04

申请号：US11625009

申请日：2007-01-19

Applicant: Jong-myeong PARK ,  Jeong-gun LEE ,  Yoon-kyoung CHO

Inventor： Jong-myeong PARK ,  Jeong-gun LEE ,  Yoon-kyoung CHO

IPC: B01L11/00

CPC classification number: F16K13/10 ,  B01L3/502715 ,  F16K31/002 ,  F16K31/025 ,  F16K99/0001 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/004 ,  F16K2099/0084 ,  Y10T436/2575

Abstract: A valve unit and an apparatus having the same include a plug which includes a phase change material in a solid state at a room temperature and a plurality of fine heat-dissipating particles dispersed in the phase change material. The fine heat-dissipating particles dispersed in the phase change material dissipate heat by absorbing an electromagnetic wave energy generated by electromagnetic wave radiation from the outside and block fluid flow in a path formed by a channel. As an external energy source irradiates an electromagnetic wave on the plug, the plurality of fine heat-dissipating particles dissipate heat and the phase change material becomes molten, thus opening the path to allow the fluid to flow.

Abstract translation: 阀单元及具有该阀单元的装置包括：塞子，其包括在室温下处于固态的相变材料和分散在相变材料中的多个细散热粒子。 分散在相变材料中的精细散热颗粒通过吸收由外部的电磁波辐射产生的电磁波能量并阻塞由通道形成的路径中的流体流而散发热量。 作为外部能量源，在电源插头上照射电磁波，多个精细的散热粒子散热，相变材料变得熔融，从而打开路径，使流体流动。

公开(公告)号：US20130178543A1

公开(公告)日：2013-07-11

申请号：US13594610

申请日：2012-08-24

Applicant: Yeon-jeong KIM ,  You-sun KIM ,  Jeong-gun LEE ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG

Inventor： Yeon-jeong KIM ,  You-sun KIM ,  Jeong-gun LEE ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG

IPC: G01N33/574 ,  C12N5/095 ,  C40B30/04 ,  C07K17/00 ,  A61P35/00 ,  A61P35/04 ,  C12N5/09 ,  G01N33/577

CPC classification number: C07K14/705 ,  C12Q1/6881 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N33/57484

Abstract: A method of detecting a cancer stem cell or circulating tumor cell that has undergone epithelial-mesenchymal transition, comprising determining the level of caveolin-1 expressed by a sample cell, and comparing the level of caveolin-1 expressed by the sample cell to a control, wherein higher expression of caveolin-1 by the sample cell indicates that the sample cell is a cancer cell, as well as a method of detecting cancer or metastasis in a subject, and related methods and compositions.

Abstract translation: 一种检测已经经历上皮 - 间质转化的癌症干细胞或循环肿瘤细胞的方法，包括测定由样品细胞表达的窖蛋白-1的水平，并将由样品细胞表达的窖蛋白-1的水平与对照组 其中样品细胞中较高表达的小窝蛋白-1表明样品细胞是癌细胞，以及检测受试者的癌症或转移的方法，以及相关的方法和组合物。