公开(公告)号：US20080151240A1

公开(公告)日：2008-06-26

申请号：US12043312

申请日：2008-03-06

Applicant: Wayne D. Roth

Inventor： Wayne D. Roth

IPC: G01J3/30 ,  G01N21/64 ,  G01N21/00

CPC classification number: B82Y20/00 ,  B82Y10/00 ,  G01N15/14

Abstract: Methods and systems for expanding the dynamic range of a system are provided. One method includes splitting fluorescent light emitted by a particle into multiple light paths having different intensities, detecting the fluorescent light in the multiple light paths with different channels to generate multiple signals, and determining which of the channels is operating in a linear range based on the multiple signals. The method also includes altering the signal generated by the channel operating in the linear range to compensate for the different intensities. Another method includes illuminating a particle in multiple illumination zones with light having different intensities and separately detecting fluorescent light emitted by the particle while located in the multiple illumination zones to generate multiple signals. The method also includes determining which of the signals is located in a linear range and altering the signal located in the linear range to compensate for the different intensities.

Abstract translation: 提供了扩展系统动态范围的方法和系统。 一种方法包括将由颗粒发射的荧光分成具有不同强度的多个光路，检测具有不同通道的多个光路中的荧光以产生多个信号，以及基于以下步骤确定哪个通道在线性范围内操作 多个信号。 该方法还包括改变由在线性范围中操作的通道产生的信号以补偿不同的强度。 另一种方法包括用具有不同强度的光照射多个照明区域中的颗粒，并分别检测位于多个照明区域中的颗粒发射的荧光，以产生多个信号。 该方法还包括确定哪个信号位于线性范围内并且改变位于线性范围内的信号以补偿不同的强度。

公开(公告)号：US20080113447A1

公开(公告)日：2008-05-15

申请号：US11938457

申请日：2007-11-12

Applicant: Jarden E. Krager ,  William R. Deicher ,  Adam Richard Schilffarth ,  Paul Pempsell ,  Wayne D. Roth

Inventor： Jarden E. Krager ,  William R. Deicher ,  Adam Richard Schilffarth ,  Paul Pempsell ,  Wayne D. Roth

IPC: G01N21/01 ,  B01J19/00 ,  G01N21/76 ,  F17D1/14

CPC classification number: G01N15/1404 ,  G01N21/6428 ,  G01N21/76 ,  G01N2015/1409 ,  Y10T137/0352 ,  Y10T137/85986 ,  Y10T436/2575

Abstract: A flow cytometer is provided which includes an interrogation flow cell and a plurality of assay fluidic lines extending into the interrogation flow cell. A method of operating such a flow cytometer includes priming the interrogation flow cell with a sheath fluid and injecting different assay fluids into a flow of the sheath fluid through the plurality of fluidic lines. A fluidic line assembly is provided which includes a plurality of capillary tubes coupled to a base section configured for coupling to an interrogation flow cell assembly of a flow cytometer. The capillary tubes are dimensionally configured such that when the fluidic line assembly is arranged within the flow cytometer and fluid is dispensed from one or more of the capillary tubes at a given pressure differential with respect to an encompassing sheath fluid within the interrogation flow cell the fluid is substantially centrally aligned within the interrogation flow cell.

Abstract translation: 提供了流式细胞仪，其包括询问流动池和延伸到询问流动池中的多个测定流体线。 操作这种流式细胞计数器的方法包括用鞘液灌注询问流动池，并将不同的分析流体注入穿过多条流体管线的鞘液流。 提供流体线组件，其包括多个毛细管，所述多个毛细管耦合到构造成耦合到流式细胞仪的询问流动池组件的基部。 毛细管的尺寸构造使得当流体线组件布置在流式细胞计数器内时，并且流体从一个或多个毛细管以相对于询问流动池内的包围护套流体的给定压差来分配时，流体 在询问流动池内基本集中对齐。

公开(公告)号：US5805236A

公开(公告)日：1998-09-08

申请号：US769016

申请日：1996-12-18

Applicant: Wayne D. Roth

Inventor： Wayne D. Roth

IPC: H04N1/00 ,  H04N5/12 ,  H04N5/20 ,  H04N5/268 ,  H04N5/14

CPC classification number: H04N1/00283 ,  H04N5/126 ,  H04N5/20 ,  H04N5/268

Abstract: Apparatus for processing analog image signals comprising: a plurality of analog image signal channels, each channel including a variable gain amplifier and a high speed video op amp connected to the variable gain amplifier, the op amp having an output and a disable/enable feature, wherein the outputs of the op amps of the plurality of signal channels are connected to a common output; and controller for controlling the plurality of signal channels so that only one channel is operable and all other channels are inoperable by setting the gain of the variable gain amplifier of the operable channel to the proper gain and by enabling the op amp of the operable channel and by setting the gains of the inoperable channels to a minimum and by disabling the op amps of the inoperable channels.

Abstract translation: 一种用于处理模拟图像信号的装置，包括：多个模拟图像信号通道，每个通道包括连接到可变增益放大器的可变增益放大器和高速视频运算放大器，该运算放大器具有输出和禁用/使能特征， 其中所述多个信号通道的运算放大器的输出连接到公共输出; 以及用于控制多个信号通道的控制器，使得通过将可操作通道的可变增益放大器的增益设置为适当增益并且通过使能可操作通道的运算放大器和仅使用可操作通道的运算放大器，使得仅一个通道可操作并且所有其它通道不可操作，以及 通过将无法使用的通道的增益设置为最小值，并禁用不可操作的通道的运算放大器。