公开(公告)号：US09212385B2

公开(公告)日：2015-12-15

申请号：US13612681

申请日：2012-09-12

Applicant: Robert H. Batchelor ,  Yue Ge ,  Iain Johnson ,  Wai-Yee Leung ,  Jixiang Liu ,  Brian Patch ,  Peter Smalley ,  Thomas Steinberg ,  Kyle Gee

Inventor： Robert H. Batchelor ,  Yue Ge ,  Iain Johnson ,  Wai-Yee Leung ,  Jixiang Liu ,  Brian Patch ,  Peter Smalley ,  Thomas Steinberg ,  Kyle Gee

IPC: A61B10/00 ,  A61B8/00 ,  A61B5/00 ,  C12Q1/28 ,  C07D265/38 ,  C07D413/12 ,  C07D495/04 ,  C09B19/00

CPC classification number: C09B19/00 ,  C07D265/38 ,  C07D413/12 ,  C07D495/04 ,  C12Q1/28

Abstract: The invention provides novel fluorinated resorufin compounds that are of use in a variety of assay formats. Also provided are methods of using the compounds and kits that include a compound of the invention and instructions detailing the use of the compound in one or more assay formats.

Abstract translation: 本发明提供可用于各种测定形式的新型氟化聚砜化合物。 还提供了使用包括本发明化合物的化合物和试剂盒的方法以及详细说明化合物在一种或多种测定形式中的用途的说明书。

公开(公告)号：US20150293110A1

公开(公告)日：2015-10-15

申请号：US14683693

申请日：2015-04-10

Applicant: SurModics IVD, Inc.

Inventor： Sean Lundquist

IPC: G01N33/58

CPC classification number: G01N33/583 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2326/10 ,  G01N33/581

Abstract: The disclosure is directed to compositions, kits, and methods for performing colorimetric analysis. A composition for a colorimetric assay can be prepared that includes an aromatic amine chromogenic substrate and a zwitterionic solubilization reagent that is N,N,N-trimethylglycine (betaine). The zwitterionic solubilization reagent is highly useful as a non-encapsulating solubilization reagent and provided faster kinetics and increased dye content of an aromatic amine chromogenic substrate in an enzymatic reaction. In turn, this can result in improvements in detection of an analyte in a sample and also can increase the sample throughput. The aromatic amine chromogenic substrate and N,N,N-trimethylglycine solubilization reagent can be used in assays such as ELISAs in order to provide a more accurate and faster detection of analytes in a biological sample.

Abstract translation: 本公开涉及用于进行比色分析的组合物，试剂盒和方法。 可以制备用于比色测定的组合物，其包括芳族胺显色底物和N，N，N-三甲基甘氨酸（甜菜碱）的两性离子溶解试剂。 两性离子溶解试剂作为非包封的增溶剂是非常有用的，并提供酶反应中芳族胺显色底物的更快的动力学和增加的染料含量。 反过来，这可以导致样品中分析物的检测的改进，并且还可以增加样品通量。 芳族胺显色底物和N，N，N-三甲基甘氨酸增溶剂可用于诸如ELISA的测定中，以便提供对生物样品中分析物的更准确和更快速的检测。

公开(公告)号：US09150837B2

公开(公告)日：2015-10-06

申请号：US13929222

申请日：2013-06-27

Applicant: Galera Therapeutics, LLC

Inventor： Daniela Salvemini ,  William L. Neumann ,  Samuel Tremont ,  Kishore Udipi ,  Amruta Reddy Poreddy

IPC: A61K47/48 ,  C12N9/02 ,  C12Q1/26 ,  C12Q1/28 ,  G01N33/50

CPC classification number: C12N9/0089 ,  A61K47/48215 ,  A61K47/60 ,  C12Q1/26 ,  C12Q1/28 ,  G01N33/5014

Abstract: Compounds and methods for utilizing compounds comprising a superoxide dismutase mimetic covalently linked to polyethylene glycol. Methods are also provided for preparing a superoxide dismutase mimetic covalently linked to a polyethylene glycol, the methods comprising reacting an activated polyethylene glycol with a superoxide dismutase mimetic, or alternatively, reacting a superoxide dismutase mimetic with an activated polyethylene glycol. A method is also provided for preventing or treating a disease or disorder in which superoxide anions are implicated, comprising administering to a subject in need thereof, a therapeutically effective amount of a compound comprising a superoxide dismutase mimetic covalently linked to a polyethylene glycol. Methods of determining the safety and efficacy of the compounds are also provided. Methods for determining the safety and efficacy can include methods in lab animals and humans.

Abstract translation: 用于利用包含与聚乙二醇共价连接的超氧化物歧化酶模拟物的化合物的化合物和方法。 还提供了制备与聚乙二醇共价连接的超氧化物歧化酶模拟物的方法，所述方法包括使活化的聚乙二醇与超氧化物歧化酶模拟物反应，或者使超氧化物歧化酶模拟物与活化的聚乙二醇反应。 还提供了一种用于预防或治疗涉及超氧化物阴离子的疾病或病症的方法，包括向有需要的受试者施用治疗有效量的包含与聚乙二醇共价连接的超氧化物歧化酶模拟物的化合物。 还提供了确定化合物的安全性和功效的方法。 确定安全性和功效的方法可以包括实验动物和人类的方法。

公开(公告)号：US20150252336A1

公开(公告)日：2015-09-10

申请号：US14675777

申请日：2015-04-01

Applicant: Massachusetts Institute of Technology

Inventor： Alice Y. Ting ,  Jeffrey Daniel Martell

IPC: C12N9/08 ,  C12Q1/28 ,  G01N33/58

CPC classification number: C12N9/0065 ,  C12Q1/28 ,  G01N33/581

Abstract: An imaging method utilizing a split peroxidase is described herein. Imaging methods involve contacting a cell with a split peroxidase and a substrate thereof to allow conversion of a substrate into a product via an enzymatic reaction catalyzed by the reconstitute split peroxidase. Also disclosed herein are split peroxidases, related products and kits.

Abstract translation: 本文描述了利用裂解过氧化物酶的成像方法。 成像方法包括使细胞与裂解的过氧化物酶及其底物接触，以通过由重构的裂解过氧化物酶催化的酶反应将底物转化成产物。 本文还公开了分离的过氧化物酶，相关产物和试剂盒。

公开(公告)号：US20150212005A1

公开(公告)日：2015-07-30

申请号：US14677524

申请日：2015-04-02

Applicant: Beckman Coulter, Inc.

Inventor： Hashem Akhavan-Tafti ,  Dean G. Binger ,  Ying Chen ,  Renuka De Silva ,  Terri McLernon ,  James Mendoza ,  Bruce H. Odegaard ,  Michael Salvati ,  Nir Shapir ,  Wenhua Xie

IPC: G01N21/76

CPC classification number: G01N21/76 ,  C09B15/00 ,  C12Q1/28 ,  G01N21/763 ,  G01N33/542 ,  G01N33/582

Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.

Abstract translation: 公开了用于确定疑似含有分析物的样品中的分析物的方法，试剂，试剂盒和系统，其中所有试剂都可溶于水溶液。 一种测定方法包括在如下条件下处理怀疑含有分析物的样品，使得如果存在分析物，则使活化剂与化学发光化合物反应构型以使其活化。 样品也用试剂处理以减少与分析物无关的信号。 最后，用触发溶液处理样品，从而产生来自活化化学发光化合物的光。 没有试剂与表面或其他固相相关。

公开(公告)号：US20150203892A1

公开(公告)日：2015-07-23

申请号：US14416984

申请日：2013-09-03

Applicant: WAKO PURE CHEMICAL INDUSTRIES, LTD. ,  OSAKA UNIVERSITY

Inventor： Shuji Matsuura ,  Nariaki Matsuura ,  Sadamu Kurono ,  Yuka Kaneko

IPC: C12Q1/527 ,  C12Q1/28 ,  C12Q1/30

CPC classification number: C12Q1/527 ,  C12Q1/28 ,  C12Q1/30 ,  G01N33/57415 ,  G01N2800/365 ,  G01N2800/52

Abstract: The present invention relates to: a breast cancer marker which is selected from the group consisting of carboxypeptidase N subunit 2, extracellular matrix protein 1, serum amyloid P component, nebulin, complement component C8 α chain, apolipoprotein L1, flavin reductase, catalase, carbonic anhydrase 2, apolipoprotein C—I, nuclear pore glycoprotein 210, superoxide dismutase [Cu—Zn], bisphosphoglycerate mutase, carbonic anhydrase 1 and peroxiredoxin-2; a method for determining breast cancer, which comprises detecting the breast cancer marker in a sample and determining breast cancer on the basis of the results of the detection; and a kit for use in the method.

Abstract translation: 本发明涉及：选自羧肽酶N亚基2，细胞外基质蛋白1，血清淀粉样蛋白P成分，神经蛋白，补体成分C8α链，载脂蛋白L1，黄素还原酶，过氧化氢酶，碳酸的乳腺癌标志物 脱水酶2，载脂蛋白C-1，核孔糖蛋白210，超氧化物歧化酶[Cu-Zn]，双磷酸甘油酸变位酶，碳酸酐酶1和过氧氧化还原酶-2; 一种确定乳腺癌的方法，其包括根据检测结果检测样品中的乳腺癌标志物并确定乳腺癌; 以及用于该方法的试剂盒。

公开(公告)号：US20150168387A1

公开(公告)日：2015-06-18

申请号：US14335010

申请日：2014-07-18

Applicant: Diagnostic Innovations, LLC

Inventor： Victor Kovalenko

IPC: G01N33/545 ,  C12Q1/28 ,  C07K17/02 ,  C07K17/08 ,  C08G81/02

CPC classification number: G01N33/545 ,  C07K17/02 ,  C07K17/08 ,  C08G81/02 ,  C12Q1/28 ,  G01N33/535 ,  G01N33/581 ,  G01N2333/908

Abstract: Provided herein are novel reagents and their use in color-producing detection systems for performing diagnostic tests and analytical assays.

Abstract translation: 本文提供了新颖的试剂及其在色彩生成检测系统中用于进行诊断测试和分析测定的用途。

公开(公告)号：US20150072367A1

公开(公告)日：2015-03-12

申请号：US14389510

申请日：2013-04-01

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Toyomi Yamaguchi

IPC: C12Q1/28

CPC classification number: C12Q1/28 ,  C12Q1/26

Abstract: Provided is a method for measuring a substance in a blood sample, which allows avoidance of the influences of both of bilirubin and hemoglobin by simple operations.Provided is a method for measuring a substance in a blood sample by an enzymatic method using an oxidizable color reagent, the method including (1) bringing the blood sample into contact with a non-ionic surfactant; and then (2) bringing the resultant sample into contact with a betaine-type amphoteric surfactant, to perform an enzyme reaction and a color reaction by an oxidizable color reagent at the same time as the contact or after the contact.

Abstract translation: 提供了一种用于测量血液样品中的物质的方法，其通过简单的操作来避免胆红素和血红蛋白的影响。 本发明提供一种通过使用可氧化显色剂的酶法测定血液样品中的物质的方法，所述方法包括（1）使血液样品与非离子表面活性剂接触; 然后（2）使所得样品与甜菜碱型两性表面活性剂接触，在接触或接触的同时进行酶反应和可氧化着色试剂的显色反应。

公开(公告)号：US20150044764A1

公开(公告)日：2015-02-12

申请号：US14523698

申请日：2014-10-24

Applicant: I-SENS, INC.

Inventor： Guen Sig Cha ,  Hakhyun Nam ,  Dongxuan Shen ,  Joo Young Cho ,  Kap Soo Park ,  Jihoon Kim

IPC: C12Q1/28 ,  G01N33/53

CPC classification number: C12Q1/28 ,  A61B5/14532 ,  A61B5/14546 ,  A61B5/1455 ,  A61B5/150022 ,  A61B5/150343 ,  A61B5/150755 ,  A61B2560/0443 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/52 ,  B01L2200/025 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2300/044 ,  B01L2300/0816 ,  B01L2400/0457 ,  G01N33/5304 ,  G01N2333/805

Abstract: The present invention relates to a biochemical assay cartridge. More particularly, the present invention provides a biochemical assay cartridge including an insertion-type solution cartridge and a reaction cartridge receiving the insertion-type solution cartridge, in which the solution cartridge is inserted into the reaction cartridge to catch a protruding protection film inducement unit by a latch in the reaction cartridge and thus break the protection film inducement unit and a protection film for sealing a reaction solution storage unit attached to the solution cartridge is automatically detached to discharge the reaction solution from the solution cartridge to the reaction cartridge. The biochemical assay cartridge according to the present invention is automatically injected into the reaction cartridge while a sample to be subjected to assay is inserted, through the insertion-type solution cartridge, into the reaction cartridge, and thus user convenience and operability of the assay cartridge are improved.

Abstract translation: 本发明涉及生化测定盒。 更具体地，本发明提供一种生物化学测定盒，其包括插入型溶液盒和接收插入型溶液盒的反应盒，其中将溶液盒插入反应盒中以通过以下步骤捕获突出的保护膜诱导单元： 反应盒中的闩锁并因此破坏保护膜诱导单元，并且自动分离用于密封附接到溶液盒的反应溶液存储单元的保护膜，以将反应溶液从溶液滤芯排出到反应盒。 根据本发明的生物化学分析盒被自动注射到反应盒中，同时待测试的样品通过插入型溶液盒插入反应盒中，因此用户方便和可操作性的测定盒 改进了

公开(公告)号：US08916341B1

公开(公告)日：2014-12-23

申请号：US14071287

申请日：2013-11-04

Applicant: Allied Innovative Systems, LLC

Inventor： Simon Bystryak ,  Rasa Santockyte

IPC: G01N21/00 ,  C12Q1/00 ,  G01N33/542 ,  C12Q1/28 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/569

CPC classification number: G01N33/542 ,  C12Q1/28 ,  G01N33/56911 ,  G01N33/56988 ,  Y02A50/52 ,  Y02A50/59

Abstract: An improved assay for detecting an analyte in a fluid sample includes a step of conducting a photochemical reaction, in which a substrate conversion catalyzed by a photosensitizer into a product of the photochemical reaction is temporary inhibited when the reaction mixture is irradiated with a light at a wavelength within a light absorption spectrum of the photosensitizer. The photosensitizer (or an enzyme to catalyze producing thereof) is attached to an entity having an affinity to the analyte, such entity is bound to the analyte prior to irradiation. The assay may increase the sensitivity of ELISA 20- to 100-fold.

Abstract translation: 用于检测流体样品中的分析物的改进测定包括进行光化学反应的步骤，其中当将光反应混合物用光照射时，由光敏剂催化的底物转化为光化学反应的产物被暂时抑制 在光敏剂的光吸收光谱内的波长。 光敏剂（或催化其产生的酶）附着于对分析物具有亲和性的实体，这样的实体在照射之前与分析物结合。 该测定可以将ELISA的灵敏度提高到100倍。