公开(公告)号：US11882829B2

公开(公告)日：2024-01-30

申请号：US17025483

申请日：2020-09-18

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson

IPC: A01N63/00 ,  A01N37/46 ,  A01N63/50 ,  A01N63/22 ,  A01N63/10 ,  A61K38/16 ,  C07K14/32 ,  C12N9/02 ,  C12N9/16 ,  C12N9/24 ,  A01N37/48 ,  C12N9/20 ,  C12N9/42 ,  C12N9/48 ,  A61K39/07

CPC classification number: A01N37/46 ,  A01N37/48 ,  A01N63/00 ,  A01N63/10 ,  A01N63/22 ,  A01N63/50 ,  A61K38/164 ,  C07K14/32 ,  C12N9/0095 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/48 ,  C12Y118/06001 ,  C12Y301/01003 ,  C12Y302/01004 ,  C12Y304/00 ,  A61K39/07 ,  C07K2319/035 ,  C12Y301/03008 ,  A01N63/50 ,  A01N63/22 ,  A01N63/50 ,  A01N63/23

Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.

公开(公告)号：US20210179672A1

公开(公告)日：2021-06-17

申请号：US17079942

申请日：2020-10-26

Applicant: Spogen Biotech Inc

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A01N37/44 ,  A61L2/18 ,  C09K8/62 ,  E21B43/16 ,  C12P21/02 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C02F3/34 ,  A23K20/147 ,  A01N63/10

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

公开(公告)号：US20210002333A1

公开(公告)日：2021-01-07

申请号：US16929422

申请日：2020-07-15

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Michelle Leslie

IPC: C07K14/32 ,  A01N37/46 ,  C12P21/02 ,  A01N63/10 ,  A01N63/50 ,  A01N63/25 ,  A01N63/22 ,  C12N1/20 ,  C07K14/195 ,  C07K14/21 ,  C07K14/245 ,  C07K14/27

Abstract: Bioactive priming polypeptides are provided that are useful when applied to plants in agricultural formulations. Methods of using the formulations containing the bioactive priming polypeptides are also provided which are applied exogenously to the surface of a plant or a plant cell membrane or endogenously to the interior of a plant or to a plant cell. The bioactive priming polypeptides when applied to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture.

公开(公告)号：US20200275664A1

公开(公告)日：2020-09-03

申请号：US16785870

申请日：2020-02-10

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson

IPC: A01N63/00 ,  A01N37/46 ,  A01N63/10 ,  C07K14/32 ,  C12N9/02 ,  C12N9/16 ,  C12N9/24 ,  A01N37/48 ,  C12N9/20 ,  C12N9/42 ,  C12N9/48

Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.

公开(公告)号：US10555534B2

公开(公告)日：2020-02-11

申请号：US16145628

申请日：2018-09-28

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson

IPC: A01N63/00 ,  C12N15/62 ,  C12N9/20 ,  A01N63/02 ,  A01N37/46 ,  C07K14/32 ,  C12N9/02 ,  C12N9/16 ,  C12N9/24 ,  A01N37/48 ,  C12N9/42 ,  C12N9/48

Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.

公开(公告)号：US10349660B2

公开(公告)日：2019-07-16

申请号：US15846487

申请日：2017-12-19

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson

IPC: A01N63/00 ,  A01N63/02 ,  A01N37/46 ,  C07K14/32 ,  C12N9/02 ,  C12N9/16 ,  C12N9/24 ,  A01N37/48 ,  C12N9/20 ,  C12N9/42 ,  C12N9/48

Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.

公开(公告)号：US09850289B2

公开(公告)日：2017-12-26

申请号：US14775892

申请日：2014-03-17

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson

IPC: C07K14/32 ,  A01N63/02 ,  A01N37/48 ,  A01N63/00 ,  C12N9/02 ,  C12N9/16 ,  C12N9/24

CPC classification number: C07K14/32 ,  A01N37/46 ,  A01N37/48 ,  A01N63/00 ,  A01N63/02 ,  C07K2319/035 ,  C12N9/0095 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/48 ,  C12Y118/06001 ,  C12Y301/01003 ,  C12Y301/03008 ,  C12Y302/01004 ,  C12Y304/00

Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.

公开(公告)号：US09845342B2

公开(公告)日：2017-12-19

申请号：US14857606

申请日：2015-09-17

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A01N37/44 ,  A61L2/18 ,  C09K8/62 ,  C02F3/34 ,  E21B43/16 ,  A23K20/147 ,  C12P21/02 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  A01N63/02 ,  C02F101/10 ,  C02F101/30 ,  A23K10/18 ,  A61K39/00 ,  C02F103/00 ,  C02F103/06 ,  C02F103/10 ,  C02F103/14 ,  C02F103/16 ,  C02F103/26 ,  C02F103/28

CPC classification number: C07K14/32 ,  A01N37/44 ,  A01N63/02 ,  A23K10/18 ,  A23K20/147 ,  A61K39/00 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K8/62 ,  C09K2208/24 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

公开(公告)号：US20170135353A1

公开(公告)日：2017-05-18

申请号：US15414050

申请日：2017-01-24

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson

IPC: A01N63/00 ,  C12N9/20 ,  C12N9/48 ,  C12N9/02 ,  C12N9/42

CPC classification number: C07K14/32 ,  A01N37/46 ,  A01N37/48 ,  A01N63/00 ,  A01N63/02 ,  C07K2319/035 ,  C12N9/0095 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/48 ,  C12Y118/06001 ,  C12Y301/01003 ,  C12Y301/03008 ,  C12Y302/01004 ,  C12Y304/00

Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.

公开(公告)号：US20140274691A1

公开(公告)日：2014-09-18

申请号：US14213238

申请日：2014-03-14

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson ,  Brittany Angle

IPC: A01N63/00

CPC classification number: A01N63/00 ,  A01H3/00 ,  A01N63/02 ,  C12R1/01 ,  C12R1/05 ,  C12R1/07 ,  C12R1/075 ,  C12R1/085 ,  C12R1/125 ,  A01N25/00

Abstract: The present invention generally relates to bacteriologically pure bacterial cultures of novel strains of plant growth-promoting bacteria, and inoculums comprising the same. The invention is also directed to plant seeds coated with the inoculums, kits comprising the inoculums and methods for stimulating plant growth by applying the biologically pure bacterial culture or the inoculum to a plant, plant seed, or plant growth medium.

Abstract translation: 本发明一般涉及植物生长促进细菌新菌株的细菌学纯细菌培养物和包含其的接种物。 本发明还涉及用接种物包被的植物种子，包含接种物的试剂盒和通过将生物学纯细菌培养物或接种物施用于植物，植物种子或植物生长培养基来刺激植物生长的方法。