公开(公告)号：US20240425840A1

公开(公告)日：2024-12-26

申请号：US18680688

申请日：2024-05-31

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Jorg Augustin ,  Ashley Siegel

IPC: C12N9/78 ,  A01N63/22 ,  A23K10/18 ,  A23K20/189 ,  A61K9/00 ,  A61K35/742 ,  A61K38/00 ,  C07K14/32 ,  C12N9/24 ,  C12N9/42

Abstract: Fusion proteins containing a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member are provided. The fusion proteins further comprise an enzyme having ACC deaminase activity, a phospholipase, a lipase, a xylanase, a xylosidase, a lactonase, a chitosanase, a protease, a glucanase, a phytase, an acid phosphatase, a pectinase, a mannanase, and/or an expansin protein. Also provided are recombinant Bacillus cereus family members that express the fusion proteins, exosporium fragments derived from the recombinant Bacillus cereus family members, and formulations containing the recombinant Bacillus cereus family members or exosporium fragments. Plant seeds treated with the recombinant Bacillus cereus family members, exosporium fragments, or formulations are also provided. The invention further relates to methods for stimulating plant growth and/or promoting plant health using the recombinant Bacillus cereus family members, exosporium fragments, or formulations.

公开(公告)号：US20180099999A1

公开(公告)日：2018-04-12

申请号：US15842062

申请日：2017-12-14

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A01N37/44 ,  A01N63/02 ,  C12N1/20 ,  A61L2/18 ,  E21B43/16 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  C09K8/62 ,  C02F3/34 ,  A61K39/00 ,  C02F103/00 ,  C02F103/06 ,  C02F103/10 ,  C02F103/14 ,  C02F103/26 ,  C02F103/28 ,  C02F103/16

CPC classification number: C07K14/32 ,  A01N37/44 ,  A01N63/02 ,  A23K10/18 ,  A23K20/147 ,  A61K39/00 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K8/62 ,  C09K2208/24 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

公开(公告)号：US20200216828A1

公开(公告)日：2020-07-09

申请号：US16648810

申请日：2018-09-20

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Jorg Augustin ,  Ashley Siegel

IPC: C12N9/78 ,  C07K14/32 ,  A01N63/22 ,  A61K9/00 ,  C12N9/42 ,  C12N9/24 ,  A61K35/742 ,  A23K10/18 ,  A23K20/189

Abstract: Fusion proteins containing a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member are provided. The fusion proteins further comprise an enzyme having ACC deaminase activity, a phospholipase, a lipase, a xylanase, a xylosidase, a lactonase, a chitosanase, a protease, a glucanase, a phytase, an acid phosphatase, a pectinase, a mannanase, and/or an expansin protein. Also provided are recombinant Bacillus cereus family members that express the fusion proteins, exosporium fragments derived from the recombinant Bacillus cereus family members, and formulations containing the recombinant Bacillus cereus family members or exosporium fragments. Plant seeds treated with the recombinant Bacillus cereus family members, exosporium fragments, or formulations are also provided. The invention further relates to methods for stimulating plant growth and/or promoting plant health using the recombinant Bacillus cereus family members, exosporium fragments, or formulations.

公开(公告)号：US12031164B2

公开(公告)日：2024-07-09

申请号：US16648810

申请日：2018-09-20

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Jorg Augustin ,  Ashley Siegel

IPC: C12N9/78 ,  A01N63/22 ,  A23K10/18 ,  A23K20/189 ,  A61K9/00 ,  A61K35/742 ,  A61K38/00 ,  C07K14/32 ,  C12N9/24 ,  C12N9/42

CPC classification number: C12N9/78 ,  A01N63/22 ,  A23K10/18 ,  A23K20/189 ,  A61K9/0056 ,  A61K35/742 ,  C07K14/32 ,  C12N9/2402 ,  C12N9/2434 ,  C12Y302/01151 ,  C12Y305/99007 ,  A61K38/00 ,  C07K2319/035

Abstract: Fusion proteins containing a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member are provided. The fusion proteins further comprise an enzyme having ACC deaminase activity, a phospholipase, a lipase, a xylanase, a xylosidase, a lactonase, a chitosanase, a protease, a glucanase, a phytase, an acid phosphatase, a pectinase, a mannanase, and/or an expansin protein. Also provided are recombinant Bacillus cereus family members that express the fusion proteins, exosporium fragments derived from the recombinant Bacillus cereus family members, and formulations containing the recombinant Bacillus cereus family members or exosporium fragments. Plant seeds treated with the recombinant Bacillus cereus family members, exosporium fragments, or formulations are also provided. The invention further relates to methods for stimulating plant growth and/or promoting plant health using the recombinant Bacillus cereus family members, exosporium fragments, or formulations.

公开(公告)号：US10407472B2

公开(公告)日：2019-09-10

申请号：US15842062

申请日：2017-12-14

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A01N37/44 ,  A61L2/18 ,  C09K8/62 ,  E21B43/16 ,  A23K20/147 ,  C12P21/02 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  A01N63/02 ,  C02F3/34 ,  A23K10/18 ,  A61K39/00 ,  C02F103/00 ,  C02F103/06 ,  C02F103/10 ,  C02F103/14 ,  C02F103/16 ,  C02F103/26 ,  C02F103/28 ,  C02F101/10 ,  C02F101/30

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

公开(公告)号：US20160108096A1

公开(公告)日：2016-04-21

申请号：US14857606

申请日：2015-09-17

Applicant: SPOGEN BIOTECH INC.

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A23K1/16 ,  C12N9/18 ,  A01N63/00 ,  E21B43/16 ,  A61L2/18 ,  A23K1/00 ,  C09K8/62 ,  C02F3/34 ,  A61K39/07 ,  A01N37/44

CPC classification number: C07K14/32 ,  A01N37/44 ,  A01N63/02 ,  A23K10/18 ,  A23K20/147 ,  A61K39/00 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K8/62 ,  C09K2208/24 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

Abstract translation: 提供了含有将融合蛋白靶向蜡状芽孢杆菌家族成员的外源的靶向序列，外切蛋白或外切蛋白片段的融合蛋白。 还提供了表达这种融合蛋白的重组蜡状芽孢杆菌家族成员。 还提供遗传灭活的蜡样芽孢杆菌家族成员和过表达外胚蛋白的重组蜡状芽孢杆菌家族成员。 还提供了用重组蜡状芽孢杆菌家族成员包被的种子和使用重组蜡状芽孢杆菌家族成员的方法（例如刺激植物生长）。 还提供了表达融合蛋白的重组蜡状芽孢杆菌家族成员的各种修饰。 还提供了包含孢子外壳蛋白和感兴趣的蛋白质或肽的融合蛋白，表达这种融合蛋白的重组细菌，用这种重组细菌包被的种子，以及使用这种重组细菌（例如刺激植物生长）的方法。

公开(公告)号：US20240199709A1

公开(公告)日：2024-06-20

申请号：US18398650

申请日：2023-12-28

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A01H3/00 ,  A01N37/44 ,  A01N63/10 ,  A01N63/20 ,  A01N63/50 ,  A23K10/18 ,  A23K20/147 ,  A61K39/00 ,  A61L2/18 ,  C02F3/34 ,  C02F101/10 ,  C02F101/30 ,  C02F103/00 ,  C02F103/06 ,  C02F103/10 ,  C02F103/14 ,  C02F103/16 ,  C02F103/26 ,  C02F103/28 ,  C09K8/62 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16

CPC classification number: C07K14/32 ,  A01H3/00 ,  A01N37/44 ,  A01N63/10 ,  A01N63/50 ,  A23K20/147 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C09K8/62 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16 ,  A01N63/20 ,  A23K10/18 ,  A61K39/00 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K2208/24

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

公开(公告)号：US11905315B2

公开(公告)日：2024-02-20

申请号：US17079942

申请日：2020-10-26

Applicant: Spogen Biotech Inc

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C12N15/62 ,  A01H3/00 ,  A01N63/20 ,  C07K14/32 ,  A01N37/44 ,  A61L2/18 ,  C09K8/62 ,  E21B43/16 ,  C12P21/02 ,  C12N1/20 ,  C12N3/00 ,  C12N15/75 ,  C02F3/34 ,  A23K20/147 ,  A01N63/50 ,  A01N63/10 ,  A61K39/00 ,  C02F103/00 ,  C02F103/06 ,  C02F103/10 ,  C02F103/14 ,  C02F103/16 ,  C02F103/26 ,  C02F103/28 ,  A23K10/18 ,  C02F101/10 ,  C02F101/30

CPC classification number: C07K14/32 ,  A01H3/00 ,  A01N37/44 ,  A01N63/10 ,  A01N63/50 ,  A23K20/147 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C09K8/62 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16 ,  A01N63/20 ,  A23K10/18 ,  A61K39/00 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K2208/24 ,  A01N63/50 ,  A01N63/22

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

公开(公告)号：US20210179672A1

公开(公告)日：2021-06-17

申请号：US17079942

申请日：2020-10-26

Applicant: Spogen Biotech Inc

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A01N37/44 ,  A61L2/18 ,  C09K8/62 ,  E21B43/16 ,  C12P21/02 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C02F3/34 ,  A23K20/147 ,  A01N63/10

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

公开(公告)号：US09845342B2

公开(公告)日：2017-12-19

申请号：US14857606

申请日：2015-09-17

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A01N37/44 ,  A61L2/18 ,  C09K8/62 ,  C02F3/34 ,  E21B43/16 ,  A23K20/147 ,  C12P21/02 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  A01N63/02 ,  C02F101/10 ,  C02F101/30 ,  A23K10/18 ,  A61K39/00 ,  C02F103/00 ,  C02F103/06 ,  C02F103/10 ,  C02F103/14 ,  C02F103/16 ,  C02F103/26 ,  C02F103/28

CPC classification number: C07K14/32 ,  A01N37/44 ,  A01N63/02 ,  A23K10/18 ,  A23K20/147 ,  A61K39/00 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K8/62 ,  C09K2208/24 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.