公开(公告)号：US20130149774A1

公开(公告)日：2013-06-13

申请号：US13615267

申请日：2012-09-13

Applicant: Min-seok KIM ,  Tae-seok SIM ,  Yeon-jeong KIM ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG ,  Jeong-gun LEE

Inventor： Min-seok KIM ,  Tae-seok SIM ,  Yeon-jeong KIM ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG ,  Jeong-gun LEE

IPC: G01N27/02 ,  C12M1/36

CPC classification number: G01N15/12 ,  C12M41/36 ,  G01N2015/0065 ,  G01N2015/1081

Abstract: A cell counter system includes an inlet via which a fluid containing a plurality of cells inflows; a channel in which the fluid flows; a valve unit, which controls flow of the fluid in the channel; an electrode unit, which is arranged in the channel for measuring impedance for counting a number of the plurality of cells; and an outlet, which is connected to the channel to drain the fluid.

Abstract translation: 细胞计数器系统包括通过其流入含有多个细胞的流体入口的入口; 流体流动的通道; 阀单元，其控制流体在通道中的流动; 电极单元，布置在通道中用于测量用于对多个单元的数量进行计数的阻抗; 和出口，其连接到通道以排出流体。

公开(公告)号：US20130137092A1

公开(公告)日：2013-05-30

申请号：US13605787

申请日：2012-09-06

Applicant: Hun-joo LEE ,  Jeong-gun LEE ,  Jeong-woo CHOI ,  Yeon-jeong KIM ,  Jong-myeon PARK ,  Mi-jeong SONG ,  Jin-mi OH

Inventor： Hun-joo LEE ,  Jeong-gun LEE ,  Jeong-woo CHOI ,  Yeon-jeong KIM ,  Jong-myeon PARK ,  Mi-jeong SONG ,  Jin-mi OH

IPC: C12M1/34 ,  G01N33/566

CPC classification number: G01N33/54306 ,  G01N33/54366

Abstract: A device, system, and method for detecting or separating target molecules allowing efficient detection even when only a small amount of target molecules or target cells are included in a sample involving the use of a target molecule linkage portion, a signal production portion, and first and second separation portions.

Abstract translation: 用于检测或分离目标分子的装置，系统和方法，即使当仅使用少量靶分子或靶细胞包括使用靶分子连接部分，信号产生部分和第一 和第二分离部。

公开(公告)号：US20130086998A1

公开(公告)日：2013-04-11

申请号：US13571202

申请日：2012-08-09

Applicant: June-young LEE ,  Sang-hyun BAEK ,  Hyo-young JEONG ,  Tae-seok SIM ,  Jeong-gun LEE

Inventor： June-young LEE ,  Sang-hyun BAEK ,  Hyo-young JEONG ,  Tae-seok SIM ,  Jeong-gun LEE

IPC: G01N1/20

CPC classification number: B01L3/502753 ,  B01L3/5027 ,  B01L2200/0621 ,  B01L2200/141 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  G01N2035/00237 ,  G01N2035/0437 ,  Y10T137/4259

Abstract: A fluid controlling apparatus including at least one sample chamber for holding a fluid containing target materials; a cleaning chamber for holding a cleaning solution; a first multi-port connected to the at least one sample chamber through a first channel and connected to the cleaning chamber through a second channel; a filter portion, connected to the first multi-port through a third channel, for filtering the target materials; and a first pump, connected to the filter portion, for applying a pressure; and a method of controlling a fluid using the fluid controlling apparatus, which comprises passing the fluid containing the target materials from the at least one sample chamber to the filter portion; and cleaning a path of the fluid by passing the cleaning solution through the path.

Abstract translation: 一种流体控制装置，包括用于保持含有目标材料的流体的至少一个样品室; 用于保持清洁溶液的清洁室; 第一多端口，其通过第一通道连接到所述至少一个样品室，并通过第二通道连接到所述清洁室; 过滤器部分，通过第三通道连接到第一多端口，用于过滤目标材料; 以及连接到过滤器部分的用于施加压力的第一泵; 以及使用所述流体控制装置控制流体的方法，所述方法包括将包含所述目标材料的流体从所述至少一个样品室传送到所述过滤器部分; 以及通过使清洁溶液通过路径来清洁流体的路径。

公开(公告)号：US20110303586A1

公开(公告)日：2011-12-15

申请号：US13156630

申请日：2011-06-09

Applicant: Tae-seok SIM ,  Jeong-gun LEE ,  Hyo-il JUNG ,  Ki-ho KWON

Inventor： Tae-seok SIM ,  Jeong-gun LEE ,  Hyo-il JUNG ,  Ki-ho KWON

IPC: B03B7/00 ,  B07B1/00

CPC classification number: B01L3/502761 ,  B01L3/502753 ,  B01L2200/0652 ,  B01L2300/087 ,  B01L2400/0487

Abstract: A device separating target particles in a fluid sample includes first through third multi-orifice flow fractionation (“MOFF”) channels, each including a multi-orifice segment with an inlet and an outlet at opposite ends, and an alternating series of contraction channels and expansion chambers interconnected in a lengthwise direction; a first separation unit including a first separation channel which is interconnected in fluid communication with a center region of the outlet of the first MOFF channel, and first branch channels which are interconnected in fluid communication with sidewall regions of the outlet of the first MOFF channel, and respectively with inlets of the second and third MOFF channels; and buffer inlets which are connected to the inlets of the second and third MOFF channels and through which a buffer flows into the second and third MOFF channels.

Abstract translation: 分离流体样品中的目标颗粒的装置包括第一至第三多孔流动分馏（“MOFF”）通道，每个通道包括具有相对端的入口和出口的多孔段，以及交替的一系列收缩通道和 膨胀室沿长度方向互相连接; 第一分离单元，包括与第一MOFF通道的出口的中心区域流体连通的第一分离通道和与第一MOFF通道的出口的侧壁区域流体连通地互连的第一分支通道， 并分别具有第二和第三MOFF通道的入口; 以及连接到第二和第三MOFF通道的入口并且缓冲器通过其流入第二和第三MOFF通道的缓冲器入口。

公开(公告)号：US20110183325A1

公开(公告)日：2011-07-28

申请号：US13079926

申请日：2011-04-05

Applicant: In-ho LEE ,  Jun-hong MIN ,  Young-rok KIM ,  Jae-ho YOU ,  Chang-eun YOO ,  Jeong-gun LEE ,  Ki-woong HAN

Inventor： In-ho LEE ,  Jun-hong MIN ,  Young-rok KIM ,  Jae-ho YOU ,  Chang-eun YOO ,  Jeong-gun LEE ,  Ki-woong HAN

IPC: C12Q1/68 ,  C12N13/00

CPC classification number: C12Q1/68 ,  C12M47/06 ,  C12N15/1006

Abstract: Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.

Abstract translation: 本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。 该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

公开(公告)号：US20110015094A1

公开(公告)日：2011-01-20

申请号：US12888927

申请日：2010-09-23

Applicant: Beom-seok LEE ,  Jeong-gun LEE ,  Hye-jung CHO ,  In-seok KANG ,  Jae-suk MOON ,  Jin-seok HONG

Inventor： Beom-seok LEE ,  Jeong-gun LEE ,  Hye-jung CHO ,  In-seok KANG ,  Jae-suk MOON ,  Jin-seok HONG

IPC: C40B40/02 ,  C40B40/06 ,  C40B40/10 ,  C40B50/18

CPC classification number: B01L3/0262 ,  B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00371 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/00689 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L2300/0819 ,  B01L2300/0838 ,  B01L2400/027 ,  Y10T436/11 ,  Y10T436/118339 ,  Y10T436/119163 ,  Y10T436/25 ,  Y10T436/2575

Abstract: Disclosed is an apparatus and method for ejecting droplets using charge concentration and liquid bridge breakup. The droplet ejection apparatus includes a reservoir storing a liquid; a capillary nozzle having a lower end submerged in the liquid stored in the reservoir and an upper end exposed outside the surface of the liquid, the capillary nozzle transferring the liquid to the upper end using capillary force; a potentiostat for applying a voltage to the liquid; a substrate mount on which a substrate is disposed to face the upper end of the capillary nozzle; and a distance adjusting unit for reciprocatingly moving the substrate between first and second positions with respect to the capillary nozzle, wherein the first position denotes a position where a distance between the upper end of the capillary nozzle and the surface of the substrate is less than a effective distance.

Abstract translation: 公开了一种使用电荷浓度和液桥分解喷射液滴的装置和方法。 液滴喷射装置包括储存液体的储液器; 毛细喷嘴，其下端浸没在存储在储存器中的液体中，上端暴露在液体表面之外，毛细喷嘴使用毛细管力将液体传送到上端; 用于向液体施加电压的恒电位仪; 基板安装件，基板设置在其上面对毛细管喷嘴的上端; 以及距离调节单元，用于使所述基板相对于所述毛细管喷嘴在第一位置和所述第二位置之间往复移动，其中所述第一位置表示所述毛细管喷嘴的上端与所述基板的表面之间的距离小于 有效距离。

公开(公告)号：US20080081357A1

公开(公告)日：2008-04-03

申请号：US11733846

申请日：2007-04-11

Applicant: Young-nam KWON ,  Jeong-gun LEE ,  Jeong-hee LEE ,  Ju-chul PARK ,  Chang-eun YOO

Inventor： Young-nam KWON ,  Jeong-gun LEE ,  Jeong-hee LEE ,  Ju-chul PARK ,  Chang-eun YOO

IPC: C12P19/34 ,  C12M1/42 ,  C12N13/00

CPC classification number: B01L3/5027 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/1861 ,  B82Y5/00 ,  B82Y30/00 ,  C12M47/06 ,  C12N1/066 ,  C12N13/00 ,  C12N15/1006

Abstract: Provided herein are a method and an apparatus for isolating nucleic acids from cells. The method comprises introducing carbon nanotubes (CNTs) and silica beads into a solution containing the cells, irradiating the solution with a laser beam disrupt the cells releasing the nucleic acids from the disrupted cells, thereby binding the nucleic acids to the silica beads, and adding a nucleic acid eluting solution to the silica beads to which the nucleic acids are bound, to elute the nucleic acids from the silica beads.

Abstract translation: 本文提供了用于从细胞中分离核酸的方法和装置。 该方法包括将碳纳米管（CNT）和二氧化硅珠粒引入到含有细胞的溶液中，用激光束照射溶液从而破坏从破碎的细胞中释放核酸的细胞，从而将核酸与二氧化硅珠结合，并加入 将与核酸结合的二氧化硅珠粒的核酸洗脱溶液从二氧化硅珠洗脱出来。

公开(公告)号：US20080058991A1

公开(公告)日：2008-03-06

申请号：US11835461

申请日：2007-08-08

Applicant: Jeong-gun LEE ,  Yoon-kyoung Cho ,  Beom-seok Lee ,  Jong-myeon Park

Inventor： Jeong-gun LEE ,  Yoon-kyoung Cho ,  Beom-seok Lee ,  Jong-myeon Park

IPC: G05B15/00 ,  B01J19/00 ,  G01N21/01

CPC classification number: B01L3/502715 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/14 ,  B01L2300/0806 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0032 ,  F16K2099/0084 ,  F16K2099/0086 ,  G01N35/00069 ,  Y10T436/11 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Provided are an apparatus and a method of controlling a microfluidic system, and the microfluidic system. The apparatus of controlling the microfluidic system includes a central control block controlling an operation of the microfluidic system, a rotator control block controlling a rotator, a position control block controlling the position of a moving unit, the moving unit moving to a position of the microfluidic structure, and a radiation energy source control block controlling energy of a radiation energy source, the radiation energy source using an electromagnetic wave to scan over a position of the microfluidic structure. Such a configuration allows effective control of a miniaturized portable microfluidic system.

Abstract translation: 提供了一种控制微流体系统和微流体系统的装置和方法。 控制微流体系统的装置包括控制微流体系统的操作的中央控制块，控制旋转器的旋转体控制块，控制移动单元的位置的位置控制块，移动到微流体位置的移动单元 结构以及辐射能量源控制块，其控制辐射能源的能量，所述辐射能源使用电磁波扫描微流体结构的位置。 这种配置允许有效地控制微型便携式微流体系统。

公开(公告)号：US20080017736A1

公开(公告)日：2008-01-24

申请号：US11781714

申请日：2007-07-23

Applicant: Beom-seok LEE ,  Jeong-gun LEE ,  Hye-jung CHO

Inventor： Beom-seok LEE ,  Jeong-gun LEE ,  Hye-jung CHO

IPC: B05B5/00

CPC classification number: B05B5/0255 ,  B01J2219/00367 ,  B01J2219/00369 ,  B01J2219/00371 ,  B01L3/0268 ,  B01L2300/0838 ,  B01L2300/161 ,  B01L2400/027 ,  B01L2400/0406 ,  B01L2400/0487 ,  B05B1/14 ,  B05B5/0533 ,  B41J2/06

Abstract: Provided is a droplet dispensing device having a nonconductive capillary nozzle. The droplet dispensing device comprises: a nonconductive capillary nozzle disposed in a downward position; a pump connected with the nonconductive capillary nozzle through a hermetically sealed fluid tube and generating a negative pressure to decrease the influence of gravity on a solution within the nonconductive capillary nozzle and the fluid tube; and an open circuit voltage supplier applying a voltage to the solution. The droplet dispensing device supplies the solution by capillary force to regularly maintain the shape of a droplet surface in the tip of the nonconductive capillary nozzle without using a separate driving device.

Abstract translation: 提供了具有非导电毛细管喷嘴的液滴分配装置。 液滴分配装置包括：设置在向下位置的非导电毛细管喷嘴; 泵，其通过气密密封的流体管与非导电毛细管喷嘴连接并产生负压，以减少重力对非导电毛细管喷嘴和流体管内的溶液的影响; 以及向该溶液施加电压的开路电压供给器。 液滴分配装置通过毛细管力提供溶液以在不使用单独的驱动装置的情况下规定地保持非导电毛细管喷嘴的尖端中的液滴表面的形状。

公开(公告)号：US20070134809A1

公开(公告)日：2007-06-14

申请号：US11610938

申请日：2006-12-14

Applicant: Yoon-kyoung CHO ,  Jeong-gun LEE ,  Sung-young JEONG

Inventor： Yoon-kyoung CHO ,  Jeong-gun LEE ,  Sung-young JEONG

IPC: G01N1/10

CPC classification number: B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/1861 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/043 ,  B01L2400/0439 ,  C12M47/02 ,  C12M47/04 ,  C12M47/06 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A microfluidic device for the concentration and lysis of cells or viruses and a method of concentrating and lysing cells or viruses using the microfluidic device include: magnetic beads, a reaction chamber in which the magnetic beads are accommodated and a laser source. The reaction chamber includes a plurality of electrodes which cross each other and are separated by a dielectric to generate an electric field and a vibrating part agitating the magnetic beads in the chamber. The laser source radiates a laser onto the magnetic beads in the reaction chamber.

Abstract translation: 用于细胞或病毒的浓缩和裂解的微流体装置以及使用微流体装置浓缩和裂解细胞或病毒的方法包括：磁珠，容纳磁珠的反应室和激光源。 反应室包括彼此交叉并被电介质隔开以产生电场的多个电极以及搅拌室中的磁珠的振动部分。 激光源将激光辐射到反应室中的磁珠上。