公开(公告)号：US11612885B2

公开(公告)日：2023-03-28

申请号：US16406816

申请日：2019-05-08

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Qi Wang ,  Matthew A. Lauber

IPC: B01J49/53 ,  B01J39/05 ,  B01D15/36 ,  B01J39/18 ,  B01J39/26 ,  B01J47/02 ,  C07K1/18 ,  C07K16/24 ,  C07K16/28 ,  C07K16/32 ,  G01N30/08 ,  B01J47/022

Abstract: The present disclosure relates to methods, compositions and kits useful for the enhanced pH gradient cation exchange chromatography of a variety of analytes. In various aspects, the present disclosure pertains to chromatographic elution buffer solutions that comprise a first buffer salt, a second buffer salt, a third buffer salt, and fourth buffer salt. The first buffer salt may be, for example, a diprotic acid buffer salt, the second buffer salt may be, for example, a divalent buffer salt with two amine groups, the third buffer salt may be, for example, a monovalent buffer salt comprising a single amine group, and the fourth buffer salt may be, for example, a zwitterionic buffer salt. Moreover, the buffer solution has a pH ranging from 3 to 11.

公开(公告)号：US20230088954A1

公开(公告)日：2023-03-23

申请号：US17881175

申请日：2022-08-04

Applicant: Waters Technologies Corporation

Inventor： Joomi Ahn ,  Moon Chul Jung ,  Kevin D. Wyndham

IPC: C12Q1/37 ,  C12N11/06 ,  C12N11/14 ,  C12M1/40 ,  C12M1/34 ,  C12Q1/48

Abstract: An immobilized enzymatic reactor can include a wall defining a chamber having an inlet and an outlet; a solid stationary phase covalently linked to an enzyme and disposed within the chamber; and a pressure modulator in a fluid communication with the chamber and adapted to support continuous flow of a liquid sample comprising a polymer analyte through the inlet, over the solid stationary phase, and out of the outlet under a pressure between about 2,500 and 35,000 psi. In one example, the solid stationary phase includes inorganic/organic hybrid particles in an ultra performance liquid chromatography system, the enzyme is a protease, and the polymer analyte is a polypeptide. The immobilized enzymatic reactor can prepare an analyte for applications such as for hydrogen deuterium exchange mass spectrometry.

公开(公告)号：US20230083910A1

公开(公告)日：2023-03-16

申请号：US17993392

申请日：2022-11-23

Applicant: Waters Technologies Corporation

Inventor： Peyton C. Beals ,  Sylvain Gilles Cormier ,  Richard Wayne Andrews

IPC: G01N30/34 ,  B01F25/432 ,  B01F33/30

Abstract: Described is a mixer for a chromatography system. The mixer includes an inlet manifold channel, an outlet manifold channel and a plurality of transfer channels. The inlet manifold channel has an inlet at a proximal end of the inlet manifold channel for receiving an inlet flow. The transfer channels are fluidly connected between the inlet and outlet manifold channels. The respective fluid connections are distributed along each of the inlet and outlet manifolds channels. The transfer channels have different volumes. The mixer may be formed of a plurality of layer and the layers may be diffusion bonded to each other.

公开(公告)号：US11597789B2

公开(公告)日：2023-03-07

申请号：US16165134

申请日：2018-10-19

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Stephan M. Koza ,  Pamela C. Iraneta ,  KEvin D. Wyndham

IPC: B01D15/30 ,  B01J20/286 ,  C08F230/08 ,  C08L33/14 ,  B01J20/32 ,  G01N30/06 ,  G01N30/02

Abstract: The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.

公开(公告)号：US11565197B2

公开(公告)日：2023-01-31

申请号：US14898014

申请日：2014-06-12

Applicant: Waters Technologies Corporation

Inventor： Christopher J. Hudalla ,  Abhijit Tarafder

IPC: B01D15/40 ,  B01D15/16 ,  G01N30/86 ,  G01N30/32 ,  B01J3/02

Abstract: A methodology scales supercritical fluid chromatography (SFC) and/or carbon dioxide based chromatography methods between different system and/or column configurations. The methodology includes measuring an average mobile phase density during a first separation utilizing C02 as a mobile phase component and substantially duplicating the average density profile for a second separation. Substantial duplication of the average mobile phase density (e.g., within about 10%, 5%, 2.5%, 1%, 0.5%, 0.1 %, 0.05%) results in chromatography for both system and/or column configurations having similar selectivity and retention factors. Average mobile phase density may be, either measured directly, calculated, or approximated using average pressure or density measurements. The average pressure profile may be used as a close approximation to duplicate average density profiles between separations.

公开(公告)号：US11554331B2

公开(公告)日：2023-01-17

申请号：US15930683

申请日：2020-05-13

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Joseph D. Michienzi ,  Joshua A. Shreve ,  Scott Kelley ,  Emily J. Berg

IPC: B01D15/14 ,  B01D15/16 ,  B01D15/40 ,  G01N30/32 ,  F16K1/38 ,  F16K25/00 ,  F15B13/02 ,  F15B13/04

Abstract: The technology generally relates to tailoring a back pressure regulator in a chromatographic system to reduce unswept volume within the back pressure regulator to achieve better sample detection and a reduction in chromatographic band distortion effects.

公开(公告)号：US20220357305A1

公开(公告)日：2022-11-10

申请号：US17864944

申请日：2022-07-14

Applicant: Waters Technologies Corporation

Inventor： Peter Kirby ,  Marcos O. Peroza ,  Steven D. Trudeau ,  Ryan M. Hill ,  Raymond P. Fisk ,  Jonathan L. Belanger ,  Wade P. Leveille

IPC: G01N30/60 ,  F16L19/00 ,  B01D15/10 ,  G01N30/02 ,  G01N30/30

Abstract: A thermal module for pre-heating liquid flowing into a liquid chromatography column, includes a trough compartment with two ends, one of the two ends having an electrical socket, a first fluidic assembly, a second fluidic assembly, and a clamp assembly. The clamp assembly includes a rail configured to receive the first fluidic assembly. The clamp assembly includes a carriage slidably mounted to the rail and configured to receive the second fluidic assembly. The carriage is operable to establish a first fluid tight seal between the first fluidic assembly and a chromatography column received within the clamp assembly, and to establish a second fluid tight seal between the second fluidic assembly and the chromatography column. The clamp assembly is disposed within the trough compartment, and the first fluidic assembly is plugged into the electrical socket at the one end of the trough compartment.

公开(公告)号：US20220357304A1

公开(公告)日：2022-11-10

申请号：US17736371

申请日：2022-05-04

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Fabrice Gritti

IPC: G01N30/46 ,  G01N30/20 ,  G01N30/72

Abstract: The exemplary embodiments may provide liquid chromatography systems that are smaller in size and with reduced extra-column volume than conventional liquid chromatography systems. The exemplary embodiments may reduce the size of the liquid chromatography systems enough that the liquid chromatography systems of the exemplary embodiments may be deployed adjacent to automated sample preparation robotics, adjacent to a process stream, or adjacent to the inlet of a mass spectrometer. In addition, the exemplary embodiments may reduce the extra-column volume of the liquid chromatography system by eliminating many of the connection tubes found in conventional liquid chromatography systems and by situating components of the liquid chromatography system in closer proximity.

公开(公告)号：US11478727B2

公开(公告)日：2022-10-25

申请号：US16848234

申请日：2020-04-14

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Jennifer M. Nguyen ,  Ximo Zhang ,  Nilini Ranbaduge ,  Robert Birdsall ,  Henry Shion ,  Melvin Blaze Muttikal Thomas ,  Thomas Walter

IPC: B01D15/16 ,  B01D15/30 ,  B01D15/38 ,  B01D3/02 ,  G01N30/72 ,  B01J20/26 ,  B01J20/283 ,  B01J20/22 ,  B01J20/285 ,  B01J20/28 ,  C07C67/54

Abstract: The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 2.50% (v/v) difluoroacetic acid and less than about 100 ppb of any individual impurity, especially metal impurities. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.

公开(公告)号：US11460438B2

公开(公告)日：2022-10-04

申请号：US16077859

申请日：2017-02-17

Applicant: Waters Technologies Corporation

Inventor： Giuseppe Astarita ,  Darryl W. Brousmiche

IPC: G01N27/622 ,  G01N27/447 ,  G01N30/06 ,  G01N30/72 ,  G01N33/58 ,  G01N33/68

Abstract: The present disclosure relates to a method for using chemical tags which have two or more sites for ionization to improve quantification and identification of components of interest from a complex mixture. This method relies on first selectively reacting one or more component in a sample with a chemical tag having two or more sites for ionization, followed by separation of components based on charge status, and finally characterization of each component to identify the same. Additionally disclosed are compounds useful as chemical tags in the disclosed methods.