公开(公告)号：US20210138361A1

公开(公告)日：2021-05-13

申请号：US17091196

申请日：2020-11-06

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Xiaoxiao Liu ,  Qi Wang ,  Nicole Lawrence

IPC: B01D15/36 ,  B01J20/283 ,  B01J20/28

Abstract: In various aspects, the present disclosure pertains to high purity chromatographic materials that comprise a chromatographic surface wherein the chromatographic surface comprises a hydrophobic modifier and an ionizable modifier comprising one or more anion exchange moieties that are positively charged when ionized, as well as devices containing such materials. In other aspects, the present disclosure provides methods for mixed mode, anion exchange reversed phase liquid chromatography comprising: (a) loading a sample comprising a plurality of acidic analytes (e.g., acidic glycans) onto a chromatographic separation device comprising such a high purity chromatographic material and (b) eluting adsorbed acidic analytes from the high purity chromatographic material with a mobile phase comprising water, organic solvent, and an organic acid salt, wherein during the course of elution a pH of the mobile phase, an ionic strength of the mobile phase, and a concentration of the organic solvent are altered over time.

公开(公告)号：US11612885B2

公开(公告)日：2023-03-28

申请号：US16406816

申请日：2019-05-08

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Qi Wang ,  Matthew A. Lauber

IPC: B01J49/53 ,  B01J39/05 ,  B01D15/36 ,  B01J39/18 ,  B01J39/26 ,  B01J47/02 ,  C07K1/18 ,  C07K16/24 ,  C07K16/28 ,  C07K16/32 ,  G01N30/08 ,  B01J47/022

Abstract: The present disclosure relates to methods, compositions and kits useful for the enhanced pH gradient cation exchange chromatography of a variety of analytes. In various aspects, the present disclosure pertains to chromatographic elution buffer solutions that comprise a first buffer salt, a second buffer salt, a third buffer salt, and fourth buffer salt. The first buffer salt may be, for example, a diprotic acid buffer salt, the second buffer salt may be, for example, a divalent buffer salt with two amine groups, the third buffer salt may be, for example, a monovalent buffer salt comprising a single amine group, and the fourth buffer salt may be, for example, a zwitterionic buffer salt. Moreover, the buffer solution has a pH ranging from 3 to 11.

公开(公告)号：US20200284771A1

公开(公告)日：2020-09-10

申请号：US16811188

申请日：2020-03-06

Applicant: Waters Technologies Corporation

Inventor： Qi Wang ,  Matthew A. Lauber ,  Samantha Ippoliti ,  Ying Qing Yu

IPC: G01N30/96 ,  H01J49/26

Abstract: The present disclosure relates to methods, compositions and kits useful for the enhanced pH gradient cation exchange chromatography of a variety of analytes. In various aspects, the present disclosure pertains to chromatographic elution kits comprising (a) a first aqueous buffer solution having a first pH and comprising a first organic acid salt in a first concentration and (b) a second aqueous buffer solution having a second pH and comprising the first organic acid salt in a second concentration, wherein the first organic acid salt comprises a first organic acid ammonium salt, wherein the second pH is greater than the first pH, and wherein the second concentration is greater than the first concentration. In various aspects, the present disclosure pertains to methods of using such aqueous buffer solutions in chromatographic separations.