公开(公告)号：US20140335553A1

公开(公告)日：2014-11-13

申请号：US14445597

申请日：2014-07-29

Applicant: Yasuhisa Asano ,  Daisuke Matsui

Inventor： Yasuhisa Asano ,  Daisuke Matsui

IPC: C12Q1/00 ,  C12Q1/28 ,  C12N9/06

CPC classification number: C12Q1/005 ,  C12N9/0022 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2533/00

Abstract: Methods are provided measuring L-lysine using a variant enzyme, an L-lysine measurement kit, and an enzyme sensor. Variant L-amino acid oxidase having a predetermined amino acid mutation, and having oxidase activity that is highly substrate-specific for L-lysine; a method for measuring L-lysine using this variant enzyme; an L-lysine measurement kit; and an enzyme sensor are also provided.

Abstract translation: 提供使用变体酶，L-赖氨酸测量试剂盒和酶传感器测量L-赖氨酸的方法。 具有预定氨基酸突变的具有对L-赖氨酸具有高度底物特异性的氧化酶活性的变体L-氨基酸氧化酶; 使用该变体酶测定L-赖氨酸的方法; L-赖氨酸测量试剂盒; 还提供酶传感器。

公开(公告)号：US08865124B2

公开(公告)日：2014-10-21

申请号：US12920283

申请日：2009-02-27

Applicant: Atsushi Miyawaki ,  Kazuki Sasaki

Inventor： Atsushi Miyawaki ,  Kazuki Sasaki

IPC: A61K51/00 ,  A61M36/14 ,  G01N33/50 ,  A61K51/08 ,  A61K49/14 ,  A61K49/00 ,  C07K14/00 ,  C12Q1/28 ,  G01N21/64 ,  G01N33/84 ,  G01N21/77

CPC classification number: G01N33/5008 ,  A61K49/0017 ,  A61K49/0056 ,  A61K49/14 ,  A61K51/08 ,  A61K51/088 ,  C07K14/00 ,  C12Q1/28 ,  G01N21/6428 ,  G01N21/77 ,  G01N33/5014 ,  G01N33/84 ,  G01N2500/10

Abstract: The present invention relates to fluorescent or luminescent probe reagents for measuring oxidative stress in a cell or an organism. Examples of the probe reagents include: a fluorescent or luminescent protein and a marker protein; a fluorescent or luminescent protein, a marker protein, and a regulatory factor; or a fluorescent or luminescent protein, a marker protein, a cleavage sequence, and a regulatory factor. In the probe reagents, the marker protein makes it possible to detect the oxidative stress caused by reactive oxygen species and comprises a regulatory factor-binding site and a ubiquitin-binding site; and the regulatory factor is a protein making it possible to regulate degradation of the marker protein in response to the reactive oxygen species. The present invention also relates to a method of measuring oxidative stress in a cell or an organism, or a method of screening a substance which suppresses or promotes the oxidative stress in a cell or an organism by using the probe reagent.

Abstract translation: 本发明涉及用于测量细胞或生物体中的氧化应激的荧光或发光探针试剂。 探针试剂的实例包括：荧光或发光蛋白和标记蛋白; 荧光或发光蛋白，标记蛋白和调节因子; 或荧光或发光蛋白，标记蛋白，切割序列和调节因子。 在探针试剂中，标记蛋白使得可以检测由活性氧引起的氧化应激，并且包含调节因子结合位点和泛素结合位点; 并且调节因子是使得可以响应于活性氧来调节标记蛋白的降解的蛋白质。 本发明还涉及一种测定细胞或生物体中的氧化应激的方法，或者通过使用探针试剂筛选抑制或促进细胞或生物体中的氧化应激的物质的方法。

公开(公告)号：US20140274931A1

公开(公告)日：2014-09-18

申请号：US14354314

申请日：2012-10-15

Applicant: Yoji Kato ,  HEALTHCARE SYSTEMS

Inventor： Yoji Kato

IPC: C07H15/207 ,  G01N33/15 ,  A61K31/235 ,  A61K31/7034 ,  C07H1/08

CPC classification number: C07H15/207 ,  A61K31/235 ,  A61K31/7034 ,  C07H1/08 ,  C07H15/203 ,  C12Q1/18 ,  C12Q1/28 ,  G01N33/15

Abstract: The present description discloses a novel biologically active ingredient of manuka honey. Specially, the present description discloses a compound represented by the following formula. In this formula, each of R1, R2 and R3 independently represents a hydrogen atom or optionally substituted C1-4 alkyl group, m represents an integer from 1 to 3, each of R4-m, R5-m and R6-m independently represents a hydrogen atom or optionally substituted C1-4 alkyl group, and each of R7, R8, R9 and R10 independently represents a hydrogen atom or optionally substituted C1-4 alkyl group.

Abstract translation: 本说明书公开了麦卢卡蜂蜜的新型生物活性成分。 特别地，本说明书公开了下式表示的化合物。 在该式中，R 1，R 2和R 3各自独立地表示氢原子或任选取代的C 1-4烷基，m表示1至3的整数，R 4-m，R 5-m和R 6-m各自独立地表示 氢原子或任意取代的C 1-4烷基，R 7，R 8，R 9和R 10各自独立地表示氢原子或任意取代的C 1-4烷基。

公开(公告)号：US08697383B2

公开(公告)日：2014-04-15

申请号：US13699422

申请日：2011-05-25

Applicant: Yasuteru Urano ,  Tetsuo Nagano ,  Tomoe Ohta ,  Fujio Yu

Inventor： Yasuteru Urano ,  Tetsuo Nagano ,  Tomoe Ohta ,  Fujio Yu

IPC: C12Q1/34 ,  C07D311/88

CPC classification number: C07D311/82 ,  C12Q1/28

Abstract: The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme.The present invention provides a compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which comprises the compound.

Abstract translation: 本发明的目的是提供一种用于检测腈相关酶的酶活性的荧光底物。 本发明提供由式（I）表示的化合物和用于检测包含该化合物的腈相关酶的酶活性的荧光底物。

公开(公告)号：US20140065653A1

公开(公告)日：2014-03-06

申请号：US13679328

申请日：2012-11-16

Applicant: California Institute of Technology

Inventor： Sebastian J. Maerkl ,  Todd A. Thorsen ,  Xiaoyan Bao ,  Stephen R. Quake ,  Vincent Studer

IPC: C12Q1/28

CPC classification number: C12Q1/28 ,  B01L3/5025 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  B81B2201/0214 ,  B81B2201/054 ,  B81B2201/07 ,  B81C1/00119 ,  F15C5/00 ,  F16K11/20 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T137/0318 ,  Y10T137/0329 ,  Y10T137/2224 ,  Y10T137/85938 ,  Y10T137/87249

Abstract: High-density microfluidic chips contain plumbing networks with thousands of micromechanical valves and hundreds of individually addressable chambers. These fluidic devices are analogous to electronic integrated circuits fabricated using large scale integration (LSI). A component of these networks is the fluidic multiplexor, which is a combinatorial array of binary valve patterns that exponentially increases the processing power of a network by allowing complex fluid manipulations with a minimal number of inputs. These integrated microfluidic networks can be used to construct a variety of highly complex microfluidic devices, for example the microfluidic analog of a comparator array, and a microfluidic memory storage device resembling electronic random access memories.

Abstract translation: 高密度微流控芯片包含具有数千个微机械阀门和数百个单独可寻址腔室的管道网络。 这些流体装置类似于使用大规模集成（LSI）制造的电子集成电路。 这些网络的一个组件是流体多路复用器，它是二进制阀模式的组合阵列，通过允许使用最少数量的输入的复杂流体操纵来指数地增加网络的处理能力。 这些集成的微流体网络可用于构建各种高度复杂的微流体装置，例如比较器阵列的微流体模拟装置和类似于电子随机存取存储器的微流体存储器存储装置。

公开(公告)号：US08557591B2

公开(公告)日：2013-10-15

申请号：US13673932

申请日：2012-11-09

Applicant: General Atomics

Inventor： Chong-Sheng Yuan ,  Abhijit Datta ,  Limin Liu

IPC: G01N33/72 ,  G01N33/00

CPC classification number: C12Q1/28 ,  G01N33/723

Abstract: The invention provides enzymatic methods for direct determination of percentage of glycated hemoglobin in blood samples without the need of a separated measurement of total hemoglobin content in blood samples. The methods utilizes one or two different types of oxidizing agents which selectively oxidize low-molecular weight reducing substances and high-molecular weight (mainly hemoglobin) reducing substances in blood samples, coupled with enzymatic reactions catalyzed by proteases, fructosyl amino acid oxidase. The amount of hydrogen peroxide generated in the reaction is measured for determination of percentage of glycated hemoglobin in blood samples. The invention provides kits for performing the methods of the invention.

Abstract translation: 本发明提供用于直接测定血液样品中糖化血红蛋白百分比的酶法，而不需要分离测量血液样品中的总血红蛋白含量。 该方法使用一种或两种不同类型的氧化剂，其选择性地氧化血液样品中的低分子量还原物质和高分子量（主要是血红蛋白）还原物质，加上由蛋白酶，果糖基氨基酸氧化酶催化的酶反应。 测定反应中产生的过氧化氢的量，以测定血液样品中糖化血红蛋白的百分比。 本发明提供了用于实施本发明方法的试剂盒。

公开(公告)号：US20130252261A1

公开(公告)日：2013-09-26

申请号：US13849056

申请日：2013-03-22

Applicant: SURMODICS, INC.

Inventor： Gary Opperman ,  Wendy Nelson

IPC: G01N21/78

CPC classification number: G01N33/581 ,  C12Q1/28 ,  G01N21/78 ,  G01N33/52 ,  G01N33/535 ,  G01N33/558

Abstract: The invention provides compositions, kits, and methods for performing colorimetric analysis. A substrate is reacted to generate a chromogenic reaction product, and a reaction stop reagent that is a sulfonic acid is added to stop and stabilize the reaction product. The absorbance properties of the chromogenic reaction product can be maintained over significantly longer periods of time of that of conventional reagents and methods. The sulfonic acid can be used in assays such as ELISAs in order to provide a more accurate and safer detection of analytes in a biological sample.

Abstract translation: 本发明提供用于进行比色分析的组合物，试剂盒和方法。 使底物反应生成显色反应产物，并加入作为磺酸的反应停止试剂以停止并稳定反应产物。 显色反应产物的吸光度特性可以在常规试剂和方法的显着更长的时间内保持。 磺酸可用于诸如ELISA的测定中，以便提供对生物样品中分析物的更准确和更安全的检测。

公开(公告)号：US20130123491A1

公开(公告)日：2013-05-16

申请号：US13811925

申请日：2011-08-09

Applicant: Haruyo Soya ,  Tomomi Murakami ,  Haruki Tsunoda ,  Yu Ohsugi ,  Ayako Yoda ,  Masashi Matsushita

Inventor： Haruyo Soya ,  Tomomi Murakami ,  Haruki Tsunoda ,  Yu Ohsugi ,  Ayako Yoda ,  Masashi Matsushita

IPC: C07D279/30

CPC classification number: C07D279/30 ,  C09B11/12 ,  C09B21/00 ,  C09B67/0077 ,  C09B67/0083 ,  C12Q1/28 ,  G01N33/723

Abstract: It is to provide a method for preserving an aqueous solution comprising a leuco chromogen, comprising adding at least one compound selected from the group consisting of polyoxyethylene alkylamine and polyoxyethylene alkenylamine to the aqueous solution containing a leuco chromogen, and a method for stabilizing a leuco chromogen, comprising allowing the leuco chromogen to coexist in an aqueous solution comprising at least one compound selected from the group consisting of polyoxyethylene alkylamine and polyoxyethylene alkenylamine. The present invention provides a method for preserving an aqueous solution comprising a leuco chromogen for stably preserving the leuco chromogen in an aqueous solution and a method for stabilizing a leuco chromogen.

Abstract translation: 本发明提供一种保存含有无色致发色素的水溶液的方法，该方法包括将至少一种选自聚氧乙烯烷基胺和聚氧乙烯链烯基胺的化合物加入到含有无色发色团的水溶液中，以及使无色发色体稳定的方法 包括使无色发色体共存于包含至少一种选自聚氧乙烯烷基胺和聚氧乙烯链烯基胺的化合物的水溶液中。 本发明提供了一种保存水溶液的方法，所述方法包括用于在水溶液中稳定保留无色发色团的无色发色体和稳定无色发色体的方法。

公开(公告)号：US20130102065A1

公开(公告)日：2013-04-25

申请号：US13447165

申请日：2012-04-14

Applicant: Tom Cheng Xu

Inventor： Tom Cheng Xu

IPC: C12Q1/28

CPC classification number: C12Q1/28 ,  G01N33/521 ,  G01N33/523 ,  G01N33/525 ,  G01N33/526

Abstract: A fast acting sensor designed to accommodate an aqueous analyte-containing sample having a volume of less than one microliter and that can be used to quantify the amount and concentration of such analyte in the sample through light reflectance or fiber-optic light reflectance. The sensor includes a storage chamber, a capillary passage, and a reaction membrane. The storage chamber acts to collect the sample and to secure such sample while it undergoes detection. The capillary passage acts to direct the sample over the reaction membrane and controls the diffusion of the sample in the storage chamber. The reaction membrane contains all of the chemicals and enzymes needed to cause a color-change reaction when contacted with the sample. The amount of analyte can be determined by light reflectance intensity with an optical measurement instrument.

Abstract translation: 一种速效传感器，其设计用于容纳体积小于1微升的含水分析物样品，可用于通过光反射率或光纤光反射率定量样品中此类分析物的量和浓度。 传感器包括储存室，毛细通道和反应膜。 储存室用于收集样品并固定样品，同时进行检测。 毛细管通道用于将样品引导到反应膜上并控制样品在储存室中的扩散。 反应膜含有当与样品接触时引起变色反应所需的所有化学物质和酶。 分析物的量可以通过光学测量仪器的光反射强度来确定。

公开(公告)号：US20130102019A1

公开(公告)日：2013-04-25

申请号：US13707725

申请日：2012-12-07

Applicant: Stanley L. Hazen ,  Renliang Zhang

Inventor： Stanley L. Hazen ,  Renliang Zhang

IPC: C12Q1/28

CPC classification number: G01N33/573 ,  C12Q1/28 ,  G01N33/6893 ,  G01N2333/908 ,  G01N2800/32 ,  G01N2800/50 ,  G01N2800/56

Abstract: Methods for characterizing the near term risk of experiencing a major adverse cardiac event in a patient presenting with chest pain are provided. In one embodiment the method comprises determining the level of myeloperoxidase (MPO) activity in a bodily sample obtained from the patient. In another embodiment, the method comprises determining the level of MPO mass in a bodily sample obtained from the patient. In another embodiment, the method comprises determining the level of one or more select MPO-generated oxidation products in a bodily sample obtained from the patient. The select MPO-generated oxidation products are dityrosine, nitrotyrosine, chlorotyrosine, methionine sulphoxide or an MPO-generated lipid peroxidation product. Levels of MPO activity, MPO mass, or the select MPO-generated oxidation product in bodily samples from the test subject are then compared to a control value that is derived from measurements of MPO activity, MPO mass, or the select MPO-generated oxidation product in comparable bodily samples obtained from control population. Such comparison can also be used to determine the near term treatment of the patient.