公开(公告)号：US4283494A

公开(公告)日：1981-08-11

申请号：US900234

申请日：1978-04-26

Applicant: Yoshitaka Kokusho ,  Haruo Machida ,  Shinjiro Iwasaki

Inventor： Yoshitaka Kokusho ,  Haruo Machida ,  Shinjiro Iwasaki

IPC: C12N9/18 ,  C12N9/20 ,  C12N1/20 ,  C12R1/05

CPC classification number: C12R1/05 ,  C12N9/18 ,  C12N9/20 ,  Y10S435/829

Abstract: A microbial lipase which has (i) an optimal pH for activity of about 9.+-.0.5, (ii) an optimal temperature for activity of about 40.degree. C. to about 48.degree. C., (iii) a lipase activity to be activated by bile salts, (iv) a cholesterol esterase activity, and (v) a molecular weight of about 30.times.10.sup.4 to about 40.times.10.sup.4 ; a process for its preparation; and microbiologically pure culture therefor.

Abstract translation: 一种微生物脂肪酶，其具有（i）约9 +/- 0.5的活性的最佳pH，（ii）约40℃至约48℃的活性的最佳温度，（iii）脂肪酶活性为 由胆汁盐活化，（iv）胆固醇酯酶活性，和（v）约30×10 4至约40×10 4的分子量; 其准备过程; 和微生物纯培养物。

公开(公告)号：US4207395A

公开(公告)日：1980-06-10

申请号：US956951

申请日：1978-11-02

Applicant: Patrick J. Cassidy ,  Jean S. Kahan

Inventor： Patrick J. Cassidy ,  Jean S. Kahan

IPC: C12P17/18

CPC classification number: C12R1/05 ,  C12P17/184

Abstract: Disclosed is a process for enzymatically cleaving the acyl group from N-acyl-6-substituted-2-substituted-1-carbadethiapen-2-em-3-carboxylic acids. The acylated substrate and the deacylated product are antibiotics.

Abstract translation: 公开了一种从N-酰基-6-取代-2-取代-1-碳代萘二烯-2-基-3-羧酸中酶切割酰基的方法。 酰化底物和脱酰基化产物是抗生素。

公开(公告)号：US20160316761A1

公开(公告)日：2016-11-03

申请号：US15211044

申请日：2016-07-15

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson ,  Brittany Angle

IPC: A01N63/00 ,  A01H3/00

CPC classification number: A01N63/00 ,  A01H3/00 ,  A01N63/02 ,  C12R1/01 ,  C12R1/05 ,  C12R1/07 ,  C12R1/075 ,  C12R1/085 ,  C12R1/125 ,  A01N25/00

Abstract: The present invention generally relates to bacteriologically pure bacterial cultures of novel strains of plant growth-promoting bacteria, and inoculums comprising the same. The invention is also directed to plant seeds coated with the inoculums, kits comprising the inoculums and methods for stimulating plant growth by applying the biologically pure bacterial culture or the inoculum to a plant, plant seed, or plant growth medium.

Abstract translation: 本发明一般涉及植物生长促进细菌新菌株的细菌学纯细菌培养物和包含其的接种物。 本发明还涉及用接种物包被的植物种子，包含接种物的试剂盒和通过将生物学纯细菌培养物或接种物施用于植物，植物种子或植物生长培养基来刺激植物生长的方法。

公开(公告)号：US07915027B2

公开(公告)日：2011-03-29

申请号：US11810731

申请日：2007-06-07

Applicant: Robin L. Brigmon ,  Sandra Story ,  Denis J. Altman ,  Christopher J. Berry

Inventor： Robin L. Brigmon ,  Sandra Story ,  Denis J. Altman ,  Christopher J. Berry

IPC: A62D3/00 ,  A62D3/02 ,  B09B3/00 ,  B09C1/10 ,  C10G32/00 ,  D06M16/00

CPC classification number: B09C1/10 ,  C12R1/01 ,  C12R1/05 ,  C12R1/40 ,  C12R1/41 ,  C12R1/425 ,  C12R1/44 ,  G21F9/18 ,  G21F9/28 ,  Y10S435/829 ,  Y10S435/874 ,  Y10S435/877 ,  Y10S435/878 ,  Y10S435/88 ,  Y10S435/882

Abstract: Novel strains of isolated and purified bacteria have been identified which have the ability to degrade petroleum hydrocarbons including a variety of PAHs. Several isolates also exhibit the ability to produce a biosurfactant. The combination of the biosurfactant-producing ability along with the ability to degrade PAHs enhances the efficiency with which PAHs may be degraded. Additionally, the biosurfactant also provides an additional ability to bind heavy metal ions for removal from a soil or aquatic environment.

Abstract translation: 已经鉴定了具有分解和纯化的细菌的新菌株，其具有降解包括各种多环芳烃在内的石油烃的能力。 几种分离物也表现出生产生物表面活性剂的能力。 生物表面活性剂生产能力的组合以及降解PAHs的能力提高了多环芳烃降解的效率。 此外，生物表面活性剂还提供了结合重金属离子以从土壤或水生环境中去除的附加能力。

公开(公告)号：US07429478B2

公开(公告)日：2008-09-30

申请号：US11024028

申请日：2004-12-28

Applicant: Rita Kumar ,  Shikha Rastogi ,  Anil Kumar

Inventor： Rita Kumar ,  Shikha Rastogi ,  Anil Kumar

IPC: C12N1/20

CPC classification number: C12R1/05 ,  C12N1/20 ,  C12R1/01 ,  C12R1/265 ,  C12R1/385 ,  C12R1/44

Abstract: The present invention provides a seeding material for accurate and reproducible monitoring of BOD load of pulp and paper wastewater, wherein the bacteria are isolated from various locations in India and comprise Micrococcus sp., Staphylococcus sp., Kurthia zopfii, Alcaligenes faecalis, and Pseudomonas aeruginosa, the microbial composition being capable of exerting an appropriate BOD for pulp and paper industrial wastewater due to the specificity of bacterial strains present therein towards the refractory organic compounds found in these effluents.

Abstract translation: 本发明提供了用于准确和可重复地监测纸浆和纸浆废水的BOD负荷的播种材料，其中细菌是从印度的不同位置分离出来的，包括微球菌属，葡萄球菌属，肉毒杆菌属，粪产碱杆菌和铜绿假单胞菌 由于其中存在的细菌菌株对这些流出物中发现的难熔有机化合物的特异性，微生物组合物能够对纸浆和造纸工业废水施加适当的BOD。

公开(公告)号：US20060141553A1

公开(公告)日：2006-06-29

申请号：US11024028

申请日：2004-12-28

Applicant: Rita Kumar ,  Shikha Rastogi ,  Anil Kumar

Inventor： Rita Kumar ,  Shikha Rastogi ,  Anil Kumar

IPC: C12Q1/04 ,  C12N1/20

CPC classification number: C12R1/05 ,  C12N1/20 ,  C12R1/01 ,  C12R1/265 ,  C12R1/385 ,  C12R1/44

Abstract: The present invention provides a seeding material for accurate and reproducible monitoring of BOD load of pulp and paper wastewater, wherein the bacteria are isolated from various locations in India and comprise Micrococcus sp., Staphylococcus sp., Kurthia zopfii, Alcaligenes faecalis, and Pseudomonas aeruginosa, the microbial composition being capable of exerting an appropriate BOD for pulp and paper industrial wastewater due to the specificity of bacterial stows present therein towards the refractory organic compounds found in these effluents.

Abstract translation: 本发明提供了用于准确和可重复地监测纸浆和纸浆废水的BOD负荷的播种材料，其中细菌是从印度的不同位置分离出来的，包括微球菌属，葡萄球菌属，肉毒杆菌属，粪产碱杆菌和铜绿假单胞菌 ，微生物组合物由于其中存在的细菌储存器的特异性而在这些流出物中发现的难熔有机化合物，因此能够为纸浆和造纸工业废水施加适当的BOD。

公开(公告)号：US6100446A

公开(公告)日：2000-08-08

申请号：US470588

申请日：1995-06-07

Applicant: Wolfgang R. Streber ,  Kenneth N. Timmis ,  Meinhart H. Zenk

Inventor： Wolfgang R. Streber ,  Kenneth N. Timmis ,  Meinhart H. Zenk

IPC: C12N15/09 ,  A01H1/04 ,  A61K39/395 ,  C12N1/20 ,  C12N1/21 ,  C12N9/02 ,  C12N15/00 ,  C12N15/01 ,  C12N15/31 ,  C12N15/82 ,  C12Q1/68 ,  C12R1/05 ,  C12R1/19 ,  C12R1/38

CPC classification number: C12N9/0004 ,  C12N15/8209 ,  C12N15/8274 ,  C12N9/0071 ,  C12R1/05

Abstract: The disclosure relates to the production and the use, by genetic engineering, of plasmids and bacterial strains containing, on a short, precisely characterizable DNA segment, the gene tfdA or a gene almost identical to tfdA.The novel plasmids and microorganisms are suitable for the production of 2,4-D (2,4-dichlorophenoxyacetic acid)-monooxygenase, and as starting materials for the transfer, by genetic engineering of the 2,4-D-degrading property of this enzyme to various organisms.

Abstract translation: 本公开涉及通过遗传工程生产和使用质粒和细菌菌株，所述质粒和细菌菌株在短的，精确表征的DNA片段上含有基因tfdA或与tfdA几乎相同的基因。 新型质粒和微生物适用于生产2,4-D（2,4-二氯苯氧基乙酸） - 单加氧酶，并且作为转移的起始原料，通过遗传工程对其进行2,4-D降解性能 酶到各种生物体。

公开(公告)号：US5527699A

公开(公告)日：1996-06-18

申请号：US396746

申请日：1995-03-01

Applicant: Masayuki Nakano ,  Hiroto Chaen ,  Toshiyuki Sugimoto ,  Toshio Miyake

Inventor： Masayuki Nakano ,  Hiroto Chaen ,  Toshiyuki Sugimoto ,  Toshio Miyake

IPC: A23G3/00 ,  A23G3/34 ,  A23G3/38 ,  A23G3/42 ,  A23L1/09 ,  A23L1/187 ,  A23L1/218 ,  A23L1/236 ,  A61K8/04 ,  A61K8/34 ,  A61K8/60 ,  A61K8/73 ,  A61Q11/00 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/02 ,  C08B37/00 ,  C12N9/26 ,  C12P19/14 ,  C12P19/20 ,  C13K13/00 ,  C12N1/00 ,  C12N1/12 ,  C12N9/28

CPC classification number: A23G3/42 ,  A23G3/346 ,  A23G3/38 ,  A23L19/20 ,  A23L27/34 ,  A23L27/35 ,  A23L29/35 ,  A23L9/12 ,  A61K8/60 ,  A61K8/732 ,  A61Q19/00 ,  C12N9/2411 ,  C12N9/2414 ,  C12P19/14 ,  C12R1/05 ,  C12Y302/01 ,  C13K13/00 ,  A23G2200/02 ,  A23G2200/06 ,  A61Q11/00 ,  A61Q17/04 ,  A61Q19/02 ,  Y10S435/829

Abstract: An amylase is preferrably obtained from Alcaligenes latus FERM BP-4578. The amylase has an activity of mainly forming maltohexaose and maltoheptaose from starch, but does not substantially have an activity of hydrolyzing maltohexaose and an oligosaccharide having a molecular weight lower than maltohexaose. The amylase does not substantially act on maltoheptaose and has a molecular weight of about 43,000+3,000 daltons as measured by sodium dodecyl sulfate polyacrylamide gel electrophoresis. It has an isoelectric point of about 7.6+0.5 as measured on isoelectrophoresis using an ampholyte, an optimum pH of about 5.0 in the presence of calcium ion, an optimum temperature of about 70.degree. C. in the presence of calcium ion as measured in terms of relative enzyme activity, a pH stability of about 4.5-10.5 in the presence of calcium ion and a thermal stability up to about 60.degree. C. in the presence of calcium ion as measured in terms of residual enzyme activity. By using the amylase, saccharide compositions rich in maltohexaose and/or maltoheptaose or those rich in maltohexaitol and/or maltoheptaitol can be readily prepared in an industrial scale, and the saccharide compositions thus obtained can be used in a variety of food products, cosmetics and pharmaceuticals.

Abstract translation: 优选从产碱杆菌FERM BP-4578获得淀粉酶。 淀粉酶主要由淀粉形成麦芽六糖和麦芽庚糖，但实质上不具有水解麦芽六糖的活性和分子量低于麦芽六糖的寡糖。 淀粉酶基本上不作用于麦芽七糖，并且通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测量，其分子量为约43,000±3,000道尔顿。 它具有约7.6±0.5的等电点，其在使用两性电解质的等电泳测量时，在钙离子存在下的最佳pH为约5.0，在钙离子存在下的最佳温度为约70℃ 相对酶活性，在钙离子存在下pH稳定性为约4.5-10.5，在钙离子存在下，在残余酶活性方面测定时，热稳定性高达约60℃。 通过使用淀粉酶，可以容易地以工业规模制备富含麦芽六糖和/或麦芽庚糖的富含麦芽六糖和/或麦芽庚糖醇的糖组合物，由此获得的糖组合物可用于各种食品，化妆品和 药品。

公开(公告)号：US5346817A

公开(公告)日：1994-09-13

申请号：US903021

申请日：1992-06-23

Applicant: Minoru Akiyama ,  Yoshiharu Doi

Inventor： Minoru Akiyama ,  Yoshiharu Doi

IPC: C12P7/62 ,  C08G63/06 ,  C12R1/05

CPC classification number: C12R1/05 ,  C12P7/625 ,  Y10S435/829

Abstract: An improved method for producing a microbial polyester comprising 3-hydroxybutyrate monomer units is disclosed, in which a strain belonging to the genus Alcaligenes is cultured in a liquid medium containing a carbon source selected from specific long chain fatty acids and derivatives thereof. The produced microbial polyester is useful in plastics and polymers which are free from environmental pollution problems, and in implanting materials and drug carriers, recovery of which is not necessary.

Abstract translation: 公开了一种用于生产包含3-羟基丁酸酯单体单元的微生物聚酯的改进方法，其中属于产碱杆菌属的菌株在含有选自特定长链脂肪酸及其衍生物的碳源的液体培养基中培养。 生产的微生物聚酯可用于没有环境污染问题的塑料和聚合物，以及植入材料和药物载体，其回收不是必需的。

公开(公告)号：US5264361A

公开(公告)日：1993-11-23

申请号：US850801

申请日：1992-03-13

Applicant: Andreas Kiener

Inventor： Andreas Kiener

IPC: C12N1/20 ,  C12P17/10 ,  C12P17/12 ,  C12R1/05 ,  C12P1/00 ,  C12P1/04

CPC classification number: C12R1/05 ,  C12P17/12 ,  Y10S435/822

Abstract: A microbiological process for the production of 6-hydroxypicolinic acid starting from 2-cyanopyridine. For this process new microorganisms are used, which are capable of growing with 2-cyanopyridine as the sole carbon, nitrogen and energy source and of converting it as the substrate into 6-hydroxypicolinic acid.

Abstract translation: 从2-氰基吡啶开始生产6-羟基吡啶甲酸的微生物过程。 对于该方法，使用新的微生物，其能够以2-氰基吡啶作为唯一的碳，氮和能源生长，并将其作为底物转化为6-羟基吡啶甲酸。