公开(公告)号：US3763000A

公开(公告)日：1973-10-02

申请号：US3763000D

申请日：1972-08-21

Applicant: TOYO JOZO KK

Inventor： ABE J ,  WATANABE T ,  YAMAGUCHI T ,  SHIBUYA Y ,  HANAMITSU K ,  FUJII T ,  MATSUMOTO K

IPC: C12P1/06 ,  C12P1/04 ,  C12P35/04 ,  C12R1/01 ,  C12R1/025 ,  C12R1/05 ,  C12R1/06 ,  C12R1/20 ,  C12D9/00

CPC classification number: C12P35/04 ,  Y10S435/824 ,  Y10S435/829 ,  Y10S435/832 ,  Y10S435/85

Abstract: Cephalexin is produced from 7-amino desacetoxy cephalosporanic acid and phenylglycine ester by enzymatic acylation with acylating enzyme derived from a microorganism selected from the group consisting of genus Alcaligenes, genus Achromobacter, genus Flavobacter, genus Bacillus and genus Beneckea.

公开(公告)号：US20250019730A1

公开(公告)日：2025-01-16

申请号：US18707448

申请日：2022-11-02

Applicant: VERSALIS S.P.A.

Inventor： Valentina RODIGHIERO ,  Alessia ERCOLE ,  Daniele RIVA ,  Alessandro DEL SEPPIA ,  Tommaso PRANDO ,  Alessandra FRATTINI

IPC: C12P7/625 ,  C12P7/10 ,  C12P7/14 ,  C12R1/05 ,  C12R1/07 ,  C12R1/185 ,  C12R1/38 ,  C12R1/865

Abstract: An integrated process for producing polyhydroxyalkanoates and bioethanol including: (a) feeding a part of the lignocellulosic hydrolyzate to a first fermentation device in the presence of one microorganism capable of using sugars with six carbon atoms and organic acids, obtaining a first fermentation broth; (b) subjecting the first broth to separation obtaining an aqueous suspension of cellular biomass having one polyhydroxyalkanoate and an aqueous phase having sugars with five carbon atoms in a quantity greater than or equal to 10 g/L; (c) optionally, feeding a part of the aqueous phase from step (b), to a second fermentation device, obtaining a second fermentation broth (inoculum); (d) feeding at least a part of the aqueous phase from step (b) and, optionally, the second broth and/or at least a part of lignocellulosic hydrolyzate, to a third fermentation device, obtaining a third fermentation broth; and (e) subjecting the third broth to separation obtaining bioethanol.

公开(公告)号：US11118159B2

公开(公告)日：2021-09-14

申请号：US16072137

申请日：2017-01-20

Applicant: King Abdullah University of Science and Technology

Inventor： Heribert Hirt ,  Axel de Zelicourt ,  Maged Saad

IPC: A01N63/20 ,  C12N1/20 ,  A01H5/10 ,  C12R1/01 ,  C12R1/05 ,  C12R1/12

Abstract: It has been discovered that the desert endophytic bacterium SA187 SA187 can provide resistance or tolerance to abiotic stress conditions to seeds or plants. Compositions containing SA187 can be used to enhance plant development and yield under environmental stress conditions.

公开(公告)号：USRE38687E1

公开(公告)日：2005-01-11

申请号：US09940941

申请日：2001-08-28

Applicant: Atsushi Sogabe ,  Takashi Hattori ,  Yoshiaki Nishiya ,  Yoshihisa Kawamura

Inventor： Atsushi Sogabe ,  Takashi Hattori ,  Yoshiaki Nishiya ,  Yoshihisa Kawamura

IPC: C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/78 ,  C12N15/00 ,  C12N15/52 ,  C12Q1/34 ,  C12R1/05 ,  C12R1/19 ,  G01N33/52

CPC classification number: G01N33/52 ,  C12Q1/34 ,  G01N2333/978 ,  Y10S435/829

Abstract: A creatine amidinohydrolase having the following physicochemical properties: Action: catalyzing the following reaction; creatine+H2O→sarcosine+urea Optimum temperature: about 40-50° C. Optimum pH: pH about 8.0-9.0 Heat stability: not more than about 50° C. (pH 7.5, 30 min) Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.0 mM Molecule weight: about 43,000 (SDS-PAGE) Isoelectric point: about 3.5 4.5, a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.

公开(公告)号：US6100446A

公开(公告)日：2000-08-08

申请号：US470588

申请日：1995-06-07

Applicant: Wolfgang R. Streber ,  Kenneth N. Timmis ,  Meinhart H. Zenk

Inventor： Wolfgang R. Streber ,  Kenneth N. Timmis ,  Meinhart H. Zenk

IPC: C12N15/09 ,  A01H1/04 ,  A61K39/395 ,  C12N1/20 ,  C12N1/21 ,  C12N9/02 ,  C12N15/00 ,  C12N15/01 ,  C12N15/31 ,  C12N15/82 ,  C12Q1/68 ,  C12R1/05 ,  C12R1/19 ,  C12R1/38

CPC classification number: C12N9/0004 ,  C12N15/8209 ,  C12N15/8274 ,  C12N9/0071 ,  C12R1/05

Abstract: The disclosure relates to the production and the use, by genetic engineering, of plasmids and bacterial strains containing, on a short, precisely characterizable DNA segment, the gene tfdA or a gene almost identical to tfdA.The novel plasmids and microorganisms are suitable for the production of 2,4-D (2,4-dichlorophenoxyacetic acid)-monooxygenase, and as starting materials for the transfer, by genetic engineering of the 2,4-D-degrading property of this enzyme to various organisms.

Abstract translation: 本公开涉及通过遗传工程生产和使用质粒和细菌菌株，所述质粒和细菌菌株在短的，精确表征的DNA片段上含有基因tfdA或与tfdA几乎相同的基因。 新型质粒和微生物适用于生产2,4-D（2,4-二氯苯氧基乙酸） - 单加氧酶，并且作为转移的起始原料，通过遗传工程对其进行2,4-D降解性能 酶到各种生物体。

公开(公告)号：US6080553A

公开(公告)日：2000-06-27

申请号：US799897

申请日：1997-02-13

Applicant: Atsushi Sogabe ,  Takashi Hattori ,  Yoshiaki Nishiya ,  Yoshihisa Kawamura

Inventor： Atsushi Sogabe ,  Takashi Hattori ,  Yoshiaki Nishiya ,  Yoshihisa Kawamura

IPC: C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/78 ,  C12N15/00 ,  C12N15/52 ,  C12Q1/34 ,  C12R1/05 ,  C12R1/19 ,  G01N33/52 ,  C12N1/00 ,  C12N1/20

CPC classification number: G01N33/52 ,  C12Q1/34 ,  G01N2333/978 ,  Y10S435/829

Abstract: A creatine amidinohydrolase having the following physicochemical properties:Action: catalyzing the following reaction;creatine+H.sub.2 O.fwdarw.sarcosine+ureaOptimum temperature: about 40-50.degree. C.Optimum pH: pH about 8.0-9.0Heat stability: not more than about 50.degree. C. (pH 7.5, 30 min)Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.0 mMMolecular weight: about 43,000 (SDS-PAGE)Isoelectric point: about 3.5,a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.

Abstract translation: 具有以下物理化学性质的肌酸脒基水解酶：作用：催化以下反应; 肌酸+ H2O->肌氨酸+尿素最佳温度：约40-50℃最佳pH：pH约8.0-9.0热稳定性：不超过约50℃（pH 7.5,30分钟）肌酸中的Km值 使用肌氨酸氧化酶和过氧化物酶的偶联试验：约3.5-10.0mM分子量：约43,000（SDS-PAGE）等电点：约3.5，一种生产所述酶的方法，包括产生所述酶的微生物的培养， 使用所述酶测定样品中的肌酸或肌酸酐及其试剂。

公开(公告)号：US5935831A

公开(公告)日：1999-08-10

申请号：US541780

申请日：1995-10-10

Applicant: Wilhelmus Johannes Quax ,  Onno Misset ,  Jan Metske Van Der Laan ,  Herman B. M. Lenting

Inventor： Wilhelmus Johannes Quax ,  Onno Misset ,  Jan Metske Van Der Laan ,  Herman B. M. Lenting

IPC: C12N1/15 ,  C12N1/21 ,  C12N9/84 ,  C12N15/09 ,  C12N15/54 ,  C12N15/55 ,  C12R1/05 ,  C12R1/19 ,  C12R1/38 ,  C12N15/01 ,  C12N9/80

CPC classification number: C12N9/84 ,  C12Y305/01011

Abstract: New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.

公开(公告)号：US5916781A

公开(公告)日：1999-06-29

申请号：US5110

申请日：1998-01-09

Applicant: Hiroaki Yamamoto ,  Kazuya Mitsuhashi ,  Akinobu Matsuyama ,  Fusao Tomita

Inventor： Hiroaki Yamamoto ,  Kazuya Mitsuhashi ,  Akinobu Matsuyama ,  Fusao Tomita

IPC: C12P13/22 ,  C12P41/00 ,  C12R1/01 ,  C12R1/05 ,  C12R1/15 ,  C12R1/18 ,  C12R1/19 ,  C12R1/22 ,  C12R1/37 ,  C12R1/40 ,  C12R1/425 ,  C12R1/64 ,  C12R1/84

CPC classification number: C12P41/001 ,  C12P13/227

Abstract: The method for producing D-tryptophan with high optical purity and high yield is provided, which comprises contacting a mixture of D,L-tryptophan with organisms which produce tryptophanase to degrade L-tryptophan selectively, thereby increasing the content of D-tryptophan in D,L-tryptophan.

Abstract translation: 提供了具有高光学纯度和高产率的D-色氨酸的制备方法，其包括使D，L-色氨酸的混合物与产生色氨酸酶的生物体接触以选择性降解L-色氨酸，从而增加D中的D-色氨酸含量 ，L-色氨酸。

公开(公告)号：US5780290A

公开(公告)日：1998-07-14

申请号：US461754

申请日：1995-06-05

Applicant: Eugene Rosenberg ,  Eliora Z. Ron

Inventor： Eugene Rosenberg ,  Eliora Z. Ron

IPC: A62D3/00 ,  A62D3/02 ,  B09C1/10 ,  C02F1/68 ,  C02F3/34 ,  C12N1/20 ,  C12N1/26 ,  C12N11/08 ,  C12R1/01 ,  C12R1/05 ,  C12R1/38 ,  C12N1/00 ,  C12N1/38

CPC classification number: A62D3/02 ,  B09C1/10 ,  C02F1/682 ,  C02F3/34 ,  C02F3/344 ,  C12N1/26 ,  A62D2101/20 ,  Y10S210/922 ,  Y10S435/821 ,  Y10S435/826 ,  Y10S435/834

Abstract: The present invention relates to compositions containing bacteria capable of degrading hydrocarbons, such as petroleum or petroleum products and methods for their use.

Abstract translation: 本发明涉及含有能够降解烃类的细菌的组合物，例如石油或石油产品及其使用方法。

公开(公告)号：US5750382A

公开(公告)日：1998-05-12

申请号：US809431

申请日：1997-06-13

Applicant: Naoaki Taoka ,  Mizuho Honda ,  Kenji Inoue ,  Kazunori Kan

Inventor： Naoaki Taoka ,  Mizuho Honda ,  Kenji Inoue ,  Kazunori Kan

IPC: C12N9/20 ,  C07C43/196 ,  C07C67/03 ,  C07C67/08 ,  C07C69/06 ,  C07C69/14 ,  C07C69/24 ,  C12P7/02 ,  C12P41/00 ,  C12R1/05 ,  C12R1/38 ,  C12R1/72 ,  C12R1/785 ,  C12P7/62 ,  C12N9/14

CPC classification number: C12P41/004 ,  C12P7/02 ,  Y10S435/829 ,  Y10S435/874 ,  Y10S435/921 ,  Y10S435/931

Abstract: A process for efficiently producing (S,S)-2-alkoxycyclohexanols in a single step by using (.+-.)-trans-2-alkoxycyclohexanols which are inexpensive and can be easily obtained. The process comprises treating a (.+-.)-trans-2-alkoxycyclohexanol with a hydrolase originating in a microorganism and being capable of esterifying stereospecifically the R-isomer in the presence of an acyl donor under such conditions that no hydrolysis occurs substantially to thereby give (S,S)-2-alkoxycyclohexanols and (R,R)-2-alkoxycyclohexanol carboxylate and then taking up the (S,S)-2-alkoxycyclohexanols.

Abstract translation: PCT No.PCT / JP96 / 02174 Sec。 371日期：1997年6月13日 102（e）日期1996年6月13日PCT 1996年8月2日PCT公布。 公开号WO97 / 06275 日期：1997年2月20日利用（+/-） - 反式-2-烷氧基环己醇，在一个步骤中有效地生产（S，S）-2-烷氧基环己醇的方法是廉价且易于获得的。 该方法包括用起源于微生物的水解酶处理（+/-） - 反式-2-烷氧基环己醇，并且在酰基供体存在下能够立即特异性酯化R-异构体的条件下，基本上不发生水解 （S，S）-2-烷氧基环己醇和（R，R）-2-烷氧基环己醇羧酸酯，然后加入（S，S）-2-烷氧基环己醇。