公开(公告)号：US06806953B2

公开(公告)日：2004-10-19

申请号：US10268159

申请日：2002-10-09

Applicant: Johann Engelhardt ,  Juergen Hoffmann

Inventor： Johann Engelhardt ,  Juergen Hoffmann

IPC: G01N2164

CPC classification number: G01N21/6458

Abstract: The invention discloses a fluorescence microscope comprising a light source that emits excitation light for illumination of a specimen, means for defining a two-dimensional search region for the excitation and detection wavelengths, means for selecting a subregion from the search region, at least one detector that detects detected light proceeding from the specimen, and a display for displaying an image of at least a portion of the specimen. Furthermore the invention discloses a method for fluorescence microscopy.

Abstract translation: 本发明公开了一种荧光显微镜，包括发射用于照射样本的激发光的光源，用于定义用于激发和检测波长的二维搜索区域的装置，用于从搜索区域选择次区域的装置，至少一个检测器 检测从样本进行的检测光，以及显示器，用于显示至少一部分样本的图像。 此外，本发明公开了一种荧光显微镜的方法。

公开(公告)号：US06754000B2

公开(公告)日：2004-06-22

申请号：US09969816

申请日：2001-10-03

Applicant: Johann Engelhardt ,  Juergen Hoffmann

Inventor： Johann Engelhardt ,  Juergen Hoffmann

IPC: G02F133

CPC classification number: G03F7/70266 ,  G02B21/002 ,  G02B26/06 ,  G02F1/33 ,  G02F2203/18 ,  G03F7/70358

Abstract: An optical arrangement having a light source, preferably a laser, for generating a light beam (1); at least one acoustooptical deflection device (3) for the light beam (1); and a correction device for correcting beam aberrations produced by the deflection device (3) is configured, in the interest of flexible and reliable correction of aberrations occurring because of the deflection, in such a way that the correction device comprises an adaptive optical system (2). Also described is a method for the deflection of light beams (1) with a light source, preferably a laser, for generating a light beam (1); at least one acoustooptical deflection device (3) for the light beam (1); and a correction device for correcting beam aberrations produced by the deflection device (3), in which an adaptive optical system (2) is used as the correction device.

Abstract translation: 一种具有用于产生光束（1）的光源（优选地是激光）的光学装置; 至少一个用于光束（1）的声光偏转装置（3）; 并且用于校正由偏转装置（3）产生的光束像差的校正装置被配置为为了灵活和可靠地校正由于偏转而出现的像差，以使得校正装置包括自适应光学系统（2 ）。 还描述了用于产生光束（1）的光源（1）与光源（优选地是激光器）的偏转的方法; 至少一个用于光束（1）的声光偏转装置（3）; 以及用于校正由偏转装置（3）产生的光束像差的校正装置，其中使用自适应光学系统（2）作为校正装置。

公开(公告)号：US06738189B1

公开(公告)日：2004-05-18

申请号：US09331189

申请日：1999-11-29

Applicant: Heinrich Ulrich ,  Johann Engelhardt

Inventor： Heinrich Ulrich ,  Johann Engelhardt

IPC: G02B2100

CPC classification number: G02B21/0024 ,  G02B21/0052 ,  G02B27/642

Abstract: The invention relates to a microscope, in particular a confocal microscope, designed to measure an object from several angular positions while avoiding any rotation of the object to be measured. The microscope has an optical device for rotation of the image to be positioned in the ray path of the microscope.

Abstract translation: 本发明涉及一种显微镜，特别是一种共聚焦显微镜，设计用于从几个角度位置测量物体，同时避免待测物体的任何旋转。 显微镜具有用于旋转图像以定位在显微镜的射线路径中的光学装置。

公开(公告)号：US06737635B2

公开(公告)日：2004-05-18

申请号：US09825273

申请日：2001-04-03

Applicant: Johann Engelhardt ,  Juergen Hoffmann ,  Rafael Storz ,  Heinrich Ulrich ,  Joerg Bewersdorf ,  Holger Birk

Inventor： Johann Engelhardt ,  Juergen Hoffmann ,  Rafael Storz ,  Heinrich Ulrich ,  Joerg Bewersdorf ,  Holger Birk

IPC: H01J4014

CPC classification number: G02B21/0068 ,  G02B21/0032 ,  G02B21/0056 ,  G02B27/283

Abstract: The present invention concerns an apparatus for combining light from at least two laser light sources, preferably in the context of confocal scanning microscopy, and in order to make laser light sources of low output power usable as light sources, in particular for confocal scanning microscopy, is characterized in that the light from the laser light sources has at least approximately the same wavelength; and that at least one beam combining unit that combines the light beams in at least largely lossless fashion is provided.

Abstract translation: 本发明涉及一种用于组合来自至少两个激光源的光的装置，优选在共聚焦扫描显微镜的上下文中，并且为了使得可用作光源的低输出功率的激光光源，特别是用于共聚焦扫描显微镜， 其特征在于，来自激光光源的光具有至少大致相同的波长; 并且提供以至少大部分无损方式组合光束的至少一个光束组合单元。

公开(公告)号：US06710918B2

公开(公告)日：2004-03-23

申请号：US09882355

申请日：2001-06-18

Applicant: Holger Birk ,  Rafael Storz ,  Johann Engelhardt ,  Kyra Moellmann

Inventor： Holger Birk ,  Rafael Storz ,  Johann Engelhardt ,  Kyra Moellmann

IPC: G02B2106

CPC classification number: B82Y20/00 ,  G02B6/02366 ,  G02B6/02371 ,  G02B6/1225 ,  G02B6/2552 ,  G02B21/002 ,  G02B21/0032 ,  G02B21/0056 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/06 ,  H01S3/005 ,  H01S3/1625 ,  H01S3/1636

Abstract: The invention discloses a scanning microscope (1) having a laser (2), which emits a light beam of a first wavelength (5, 43, 53) and is directed onto an optical element (9) that modifies the wavelength of the light beam at least to some extent. Means (16) for suppressing the light of the first wavelength in the modified-wavelength light beam (5, 47, 57) are provided.

Abstract translation: 本发明公开了一种具有激光器（2）的扫描显微镜（1），其发射第一波长（5,43,53）的光束并被引导到光学元件（9）上，所述光学元件（9）修改光束的波长 至少在一定程度上。 提供了用于抑制修改波长光束（5,47,57）中的第一波长的光的装置（16）。

公开(公告)号：US06686583B2

公开(公告)日：2004-02-03

申请号：US09901900

申请日：2001-07-11

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: H01J314

CPC classification number: G02B21/0052 ,  G02B21/0032 ,  G02F1/113

Abstract: The present invention concerns an apparatus for coupling light (1) of at least one wavelength of a laser light source (2) into an optical assemblage (3), preferably into a confocal scanning microscope, having an optically active component (4) that serves in particular to select the wavelength and to set the power of the coupled-in light (5). To ensure that changes in the power and/or wavelength of the laser light source do not affect the power of the light (5) coupled into the optical assemblage, the apparatus according to the present invention is characterized in that in order to influence the coupled-in light (5), the component (4) serves as the adjusting element of a control system (11).

Abstract translation: 本发明涉及一种用于将激光源（2）的至少一个波长的光（1）耦合到光学组件（3）中的装置，优选地将共焦扫描显微镜耦合到具有光学活性部件（4）的装置 特别是选择波长并设置耦合光的功率（5）。 为了确保激光光源的功率和/或波长的变化不影响耦合到光学组件中的光（5）的功率，根据本发明的装置的特征在于，为了影响耦合 在光（5）中，组件（4）用作控制系统（11）的调节元件。

公开(公告)号：US06680796B2

公开(公告)日：2004-01-20

申请号：US09887546

申请日：2001-06-25

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G02B2106

CPC classification number: G02B21/082 ,  G02B21/0032 ,  G02B21/32

Abstract: A microscope assemblage, in particular a confocal laser scanning microscope, has a light source (1), for illumination of a specimen (6). An objective (5) is provided for guiding the illuminating light beam to the specimen (6). A device, having a tilt element, is provided for generating a relative motion between the illuminating light beam and the specimen (6). The device and the tilt are configured so that the illuminating light beam is rotatable in the objective pupil.

Abstract translation: 显微镜组合件，特别是共聚焦激光扫描显微镜，具有用于照射样品（6）的光源（1）。 提供了一种用于将照明光束引导到样本（6）的目标（5）。 提供具有倾斜元件的装置，用于产生照明光束和样本（6）之间的相对运动。 设备和倾斜被配置为使得照明光束能够在目标光瞳中旋转。

公开(公告)号：US06677596B2

公开(公告)日：2004-01-13

申请号：US09948297

申请日：2001-09-06

Applicant: Johann Engelhardt ,  Juergen Hoffmann

Inventor： Johann Engelhardt ,  Juergen Hoffmann

IPC: G01J328

CPC classification number: G01N21/6458 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/16

Abstract: A method for detection of fluorescent light in scanning microscopy includes dividing an illuminating light beam into a plurality of partial illuminating beams so as to illuminate multiple specimen regions simultaneously. Fluorescing materials in a specimen region are excited via multi-photon excitation. Operating parameters of the light source are adapted for optimum fluorescent photon yield to the properties of the fluorescing material in the specimen region. Fluorescent light of the specimen regions is detected simultaneously.

Abstract translation: 在扫描显微镜中检测荧光的方法包括将照明光束分成多个部分照明光束，以同时照射多个标本区域。 通过多光子激发激发样品区域中的荧光材料。 光源的操作参数适用于使样品区域中荧光材料的特性达到最佳的荧光光子产量。 同时检测样品区域的荧光。

公开(公告)号：US06654166B2

公开(公告)日：2003-11-25

申请号：US09881047

申请日：2001-06-15

Applicant: Holger Birk ,  Rafael Storz ,  Johann Engelhardt

Inventor： Holger Birk ,  Rafael Storz ,  Johann Engelhardt

IPC: G02B2106

CPC classification number: B82Y20/00 ,  G02B6/02295 ,  G02B6/1225 ,  G02B6/2552 ,  G02B21/002 ,  G02B21/0032 ,  G02B21/0056 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/06 ,  H01S3/005 ,  H01S3/1625 ,  H01S3/1636

Abstract: A scanning microscope is disclosed, through which a sample (14) can be illuminated and detected. An illumination pinhole and a detection pinhole (10, 16) are respectively arranged in the illumination beam path and in the detection beam path (8, 15), an optical component (4), which generates at least to some extent spectrally broadened illumination light, is provided in the illumination beam path (8). A polarization-independent and wavelength-independent beam splitter (11) is arranged in a fixed position in the illumination beam path and the detection beam path (8, 15).

公开(公告)号：US06618178B2

公开(公告)日：2003-09-09

申请号：US09901274

申请日：2001-07-09

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G02B2608

CPC classification number: G02B21/0048

Abstract: The present invention relates to an optical arrangement for deflecting a light beam (1, 14), in particular in two substantially mutually perpendicular directions (2, 3), preferably for applying to confocal scanning microscopes, having two mirrors (8, 10) which can be rotated by means of a rotary drive (4, 5) in each case about mutually perpendicular axes—the x-axis (6) and y-axis (7)—one of the two mirrors (8, 10) being assigned a further mirror (9) in a prescribed angular position in a rotationally fixed fashion such that the mutually assigned mirrors (8, 9)—first and second mirrors—rotate jointly about the y-axis (7), and in so doing rotate the light beam (1, 14) about a pivot (11) which lies on the axis of rotation (6)—the x-axis—of the third mirror (10). In order to minimize and, in the ideal case, to eliminate the distortion errors produced by the arrangement, the optical arrangement is characterized in that the mirrors (8, 9, 10) are arranged in such a way that the optical axis of the light beam (12) running between the second and the third mirrors (9, 10) always lies substantially in a plane containing the x-axis (6) and perpendicular to the y-axis (7).