公开(公告)号：US06614525B1

公开(公告)日：2003-09-02

申请号：US09718948

申请日：2000-11-20

Applicant: Johann Engelhardt ,  Joachim Bradl

Inventor： Johann Engelhardt ,  Joachim Bradl

IPC: G01J330

CPC classification number: G02B21/0032 ,  G02B21/0064

Abstract: A laser scanning microscope, preferably a confocal laser scanning microscope, having a laser light source for illuminating a specimen and a detector for detecting the light returning from the specimen, the specimen or parts thereof. The specimen is marked with markers that can be excited to emit. For the specific detection of preferably biological specimen structures, with high localization accuracy for the specimen structures, the laser light source emits exciting light substantially at one wavelength. Different markers emit light of different wavelengths, when irradiated with exciting light of substantially the same wavelength. The detector is embodied as a multi-band detector for the simultaneous detection of light at several wavelengths. A corresponding method for the detection of preferably biological specimens or specimen structures by laser scanning microscopy is described.

Abstract translation: 激光扫描显微镜，优选共焦激光扫描显微镜，具有用于照射样本的激光光源和用于检测从样本返回的光的检测器，其样本或其部分。 标本上标有可被激发发射的标记。 对于优选生物样本结构的具体检测，对于样本结构具有高定位精度，激光光源基本上发射一个波长的激发光。 当用基本上相同波长的激发光照射时，不同的标记发射不同波长的光。 检测器被实现为用于同时检测几个波长的光的多波段检测器。 描述了通过激光扫描显微镜检测优选生物样品或样本结构的相应方法。

公开(公告)号：US06614031B2

公开(公告)日：2003-09-02

申请号：US09946695

申请日：2001-09-04

Applicant: Johann Engelhardt ,  Juergen Hoffmann ,  Werner Knebel

Inventor： Johann Engelhardt ,  Juergen Hoffmann ,  Werner Knebel

IPC: H01N21268

CPC classification number: G01N21/6458 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/008

Abstract: A method for examining a specimen (11) by means of a confocal scanning microscope having at least one light source (1), preferably a laser, to generate an illuminating light beam (4) for the specimen (11), and a beam deflection device (9) to guide the illuminating light beam (4) over the specimen (11) comprises, in the interest of reliable definition of details or regions of interest of the specimen (11), the following method steps: Firstly a preview image is acquired. Then at least one region of interest in the preview image is marked. This is followed by allocation of individual illuminating light beam wavelengths and/or illuminating light beam power levels to the region or regions. Illumination of the region or regions of the specimen (11) in accordance with the allocation is then accomplished, at least one manipulation in at least one region (25) being performed by means of the illumination. Also described is a confocal scanning microscope having at least one light source (1), preferably a laser, to generate an illuminating light beam (4) for a specimen (11), and a beam deflection device (9) to guide the illuminating light beam (4) over the specimen (11), means for acquiring a preview image and means for marking at least one region of interest in the preview image being provided, such that individual illuminating light beam wavelengths and/or illuminating light beam power levels can be allocated to the region or regions, and the region or regions of the specimen (11) can be illuminated in accordance with the allocation, and such that at least one manipulation in at least one region (25) can be performed by means of the illumination.

公开(公告)号：US06608295B2

公开(公告)日：2003-08-19

申请号：US10106533

申请日：2002-03-26

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G02B2740

CPC classification number: G02B21/002

Abstract: A scanning microscope has at least one illumination source for emitting a light beam, which is fed via a microscope optic to a specimen and scans the latter. In order to correct the imaging defect of the microscope optic, said defect is determined and a correction value is determined therefrom. This correction value is used for influencing control signals which control the impinging of the light beam on the specimen.

公开(公告)号：US06580554B2

公开(公告)日：2003-06-17

申请号：US09974014

申请日：2001-10-10

Applicant: Johann Engelhardt ,  Werner Knebel

Inventor： Johann Engelhardt ,  Werner Knebel

IPC: G02B2100

CPC classification number: G02B21/002

Abstract: A method and an arrangement for beam control in a scanning microscope are disclosed. The scanning microscope comprises means for acquiring and displaying (3) a preview image (7) and a microscope optical system (51). Means for marking (5) at least one region of interest (27, 29) in the preview image (7) are provided. A first beam deflection device (43, 67, 68) displaces the scan field (31, 33) onto the region of interest (27, 29); and a second beam deflection device (49, 72, 94) serves for meander-shaped scanning within the scan field (31, 33).

公开(公告)号：US06496307B2

公开(公告)日：2002-12-17

申请号：US09770802

申请日：2001-01-26

Applicant: Johann Engelhardt ,  Heinrich Ulrich

Inventor： Johann Engelhardt ,  Heinrich Ulrich

IPC: G02B2106

CPC classification number: G02B21/0032 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/18

Abstract: A confocal scanning microscope, having a light source (1) for illuminating an object (6), which is to be investigated, with exciting light (2), at least two detection channels exhibiting detection light (8, 9) being produced, is configured with regard to a high signal yield and a high signal-to-noise ratio in such a way that at least two detection channels can be optically superimposed by means of a superimposing device (11, 12,13, 15,17, 18).

Abstract translation: 一种共焦扫描显微镜，其具有用于用激发光（2）照亮待研究的物体（6）的光源（1），产生显示出检测光（8,9）的至少两个检测通道。 关于高信号产量和高信噪比被配置，使得至少两个检测通道可以通过叠加装置（11,12,13,15,17,18）被光学地叠加， 。

公开(公告)号：US06444971B1

公开(公告)日：2002-09-03

申请号：US09476649

申请日：1999-12-31

Applicant: Johann Engelhardt ,  Bernd Widzgowski

Inventor： Johann Engelhardt ,  Bernd Widzgowski

IPC: G01N2100

CPC classification number: G02B21/0084 ,  G02B21/008 ,  G02B21/06

Abstract: A method and system for compensating intensity fluctuations of an illumination system in a confocal microscope comprise a first and a second analog-to-digital converters for digitizing a first electrical signal corresponding to the light reflected from a specimen, and for digitizing a second electrical signal corresponding to an illumination reference, respectively. The digitized signals are sent to a first and a second look up tables carrying out a log conversion of the first and second electrical signals, respectively. Also provided is a calculator for correcting the first electrical signal for intensity fluctuations of the second electrical signal. The corrected electrical signal is sent to a third look up table for converting the corrected electric signal. The conversion is done by exponentiation of the corrected electrical signal.

Abstract translation: 一种用于补偿共焦显微镜中的照明系统的强度波动的方法和系统，包括第一和第二模拟数字转换器，用于数字化对应于从样本反射的光的第一电信号，以及用于数字化第二电信号 分别对应于照明参考。 数字化信号被发送到分别执行第一和第二电信号的对数转换的第一和第二查询表。 还提供了一种用于校正第一电信号以用于第二电信号的强度波动的计算器。 校正后的电信号被发送到第三查询表，用于转换校正的电信号。 通过校正电信号的取幂来进行转换。

公开(公告)号：US06433814B1

公开(公告)日：2002-08-13

申请号：US09331459

申请日：1999-09-03

Applicant: Johann Engelhardt ,  Martin Hoppe

Inventor： Johann Engelhardt ,  Martin Hoppe

IPC: H04N718

CPC classification number: G02B21/36 ,  G02B21/0024

Abstract: A TV camera for microscopic or macroscopic imaging is characterized by a confocal lens with a laser light source (1), a scanner (2), a detector (3) and an electronic control unit (4), for using confocal characteristics in the most compact possible format.

Abstract translation: 用于微观或宏观成像的电视摄像机的特征在于具有激光光源（1），扫描仪（2），检测器（3）和电子控制单元（4）的共焦透镜，用于最多使用共焦特征 紧凑可能的格式。

公开(公告)号：US08586945B2

公开(公告)日：2013-11-19

申请号：US13297872

申请日：2011-11-16

Applicant: Matthias Reuss ,  Johann Engelhardt

Inventor： Matthias Reuss ,  Johann Engelhardt

IPC: G01J1/58

CPC classification number: G02B21/0032 ,  G02B21/0068 ,  G02B21/0076

Abstract: A fluorescence light scanning microscope (2) comprises a light source providing excitation light (8) for exciting a fluorophore in a sample to be imaged for spontaneous emission of fluorescence light, and suppression light (7) for suppressing spontaneous emission of fluorescence light by the fluorophore on a common optical axis (4), the suppression wavelength differing from the excitation wavelength; an objective (19) focusing both the excitation (8) and the suppression (7) light to a focus point; a detector (21) detecting fluorescence light (11) spontaneously emitted by the fluorophore; and a chromatic beam shaping device (1) arranged on the common optical axis (4), and including a birefringent chromatic optical element (3) adapted to shape a polarization distribution of the suppression light (7) such as to produce an intensity zero at the focus point, and to leave the excitation light such as to produce a maximum at the focus point.

Abstract translation: 荧光光扫描显微镜（2）包括提供用于激发要成像的样品中的荧光团以激发荧光的自发发射的激发光（8）的光源，以及用于抑制荧光的自发发射的抑制光（7） 荧光团在共同的光轴（4）上，抑制波长与激发波长不同; 将激发（8）和抑制（7）光聚焦到焦点的目标（19） 检测器（21），检测由荧光团自发发射的荧光（11）; 以及布置在公共光轴（4）上的色光束整形装置（1），并且包括适于使抑制光（7）的偏振分布成形的双折射彩色光学元件（3），以产生在 焦点，并留下激发光，以便在焦点处产生最大值。

公开(公告)号：US20120212750A1

公开(公告)日：2012-08-23

申请号：US13441043

申请日：2012-04-06

Applicant: Johann Engelhardt ,  Stefan W. Hell ,  Jan Keller-Findeisen

Inventor： Johann Engelhardt ,  Stefan W. Hell ,  Jan Keller-Findeisen

IPC: G01B11/14

CPC classification number: G02B21/0076 ,  G01N21/6456 ,  G01N2021/6463

Abstract: To the end of three-dimensionally localizing light emitting marker entities of unknown orientation and unknown position in a sample, the light emitted by each single marker entity is imaged in at least two different ways onto at least one detection plane which corresponds to a focal plane (13) in the sample resulting in at least two images of the marker entity. Virtual x- and y-positions of the marker entity in parallel to the focal plane (13) are separately determined from the emitted light intensity distribution over each image of the marker entity. Further, the z-position of the marker entity normal to the focal plane is determined from the emitted light intensity distributions over the images of the marker entity. The real x- and y-positions of the marker entity in parallel to the focal plane (13) are determined based on its virtual x- and y-positions and on its z-position.

Abstract translation: 在三维定位样品中未知取向和未知位置的发光标记实体的三维定位结束时，由每个单个标记实体发射的光以至少两种不同的方式成像到至少一个对应于焦平面的检测平面 （13），导致标记实体的至少两个图像。 标记实体与焦平面（13）平行的虚拟x和y位置根据标记实体的每个图像上的发射光强度分布分别确定。 此外，从标记实体的图像上的发射光强度分布确定垂直于焦平面的标记实体的z位置。 标记实体与焦平面（13）平行的真实x和y位置基于其虚拟x和y位置及其z位置来确定。

公开(公告)号：US20080165358A1

公开(公告)日：2008-07-10

申请号：US11910938

申请日：2006-03-31

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G02B27/28 ,  G01J4/00

CPC classification number: G02B21/0076 ,  G02B21/0036

Abstract: The present invention relates to an optical scanning device that comprises a light source to emit a beam of light, and a beam splitter to split that beam into several beamlets, and further a first objective lens to direct said beamlets onto a focal plane wherein each of said beamlets impinges on the focal plane spacially separated from each other, wherein the beam splitter comprises several birefringent elements for splitting said beam, preferably a stack of Wollaston prisms.

Abstract translation: 本发明涉及一种包括发射光束的光源的光学扫描装置，以及将该光束分成几个子束的分束器，以及另外的第一物镜，以将所述子束引导到焦平面上，其中 所述子束照射在彼此空间上分离的焦平面上，其中分束器包括若干双折射元件，用于分裂所述光束，优选地是Wollaston棱镜的堆叠。