公开(公告)号：US20160061811A1

公开(公告)日：2016-03-03

申请号：US14783494

申请日：2014-04-11

Applicant: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO

Inventor： Shana Olwyn KELLEY ,  Edward Hartley SARGENT ,  Mohamad Reza MOHAMADI

IPC: G01N33/49 ,  G01N27/00

CPC classification number: G01N33/491 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C1/01 ,  B03C1/0335 ,  B03C1/034 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/26 ,  G01N1/4077 ,  G01N15/00 ,  G01N15/0606 ,  G01N15/0656 ,  G01N27/00 ,  G01N33/54326 ,  G01N33/57434 ,  G01N2015/0053 ,  G01N2015/0065

Abstract: Devices and methods for capture of target particles in a flow. There is a plurality of flow rate-reducing structures in a flow chamber, each structure including a trapping surface shaped to reduce flow rate in a vicinity of the trapping surface. Reduced flow rate in the vicinity of the trapping surface is non-zero and has a magnitude lower than that of flow rate in other regions of the flow chamber. The reduced flow rate is sufficiently low for an attraction force acting on the target particles to overcome drag force on the target particles, to promote capture of particles in the vicinity of the trapping surface. The device may exhibit different sorting zones for capturing particles that experience different amounts and/or types of attraction force. The device may enable sorting of cells according to their level of display of specific protein surface markers.

Abstract translation: 在流中捕获目标颗粒的装置和方法。 在流动室中存在多个流量减小结构，每个结构包括成形为减小捕获表面附近的流速的捕获表面。 捕集表面附近的流量减少非零，并且具有比流动室的其它区域中的流速低的量值。 对于作用在目标颗粒上的吸引力，降低的流速足够低以克服目标颗粒上的阻力，以促进捕获表面附近的颗粒捕获。 该装置可以展示不同的分选区域，用于捕获经历不同量和/或类型的吸引力的颗粒。 该装置可以使细胞根据其特异性蛋白质表面标记物的显示水平进行分选。

公开(公告)号：US09274131B2

公开(公告)日：2016-03-01

申请号：US14293661

申请日：2014-06-02

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Shao-Hsing Yeh ,  Yu-Ying Lin ,  Chi-Han Chiou ,  Liang-Ju Chien

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N35/00 ,  B03C1/01 ,  B03C1/033 ,  B03C1/035 ,  B03C1/28 ,  B01L3/00

CPC classification number: G01N35/0098 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2300/087 ,  B01L2400/043 ,  B03C1/01 ,  B03C1/0335 ,  B03C1/035 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N1/28 ,  Y10T137/0391 ,  Y10T137/206 ,  Y10T137/2191 ,  Y10T137/2224

Abstract: The disclosure relates to a droplet manipulating device and a method for manipulating a droplet. The droplets manipulating device includes a first magnetic field generator, a second magnetic field generator, and a flow channel. The first magnetic field generator produces a first magnetic field on the droplet, so that the droplet has the direction of magnetic field corresponding to the first magnetic field. Further, the second magnetic field generator produces a second magnetic field on the droplet so as to drive the droplet to be in motion in the flow channel.

Abstract translation: 本发明涉及液滴操纵装置和操纵液滴的方法。 液滴操纵装置包括第一磁场发生器，第二磁场发生器和流动通道。 第一磁场发生器在液滴上产生第一磁场，使得液滴具有对应于第一磁场的磁场方向。 此外，第二磁场发生器在液滴上产生第二磁场，以便驱动液滴在流动通道中运动。

公开(公告)号：US09274032B2

公开(公告)日：2016-03-01

申请号：US13873072

申请日：2013-04-29

Applicant: BioControl Systems, Inc.

Inventor： Matti Korpela ,  Kenneth Rundt

IPC: B03C1/00 ,  H01F7/04 ,  G01N1/28 ,  B03C1/28 ,  G01N33/543

CPC classification number: G01N1/28 ,  B03C1/286 ,  B03C2201/18 ,  B03C2201/26 ,  G01N33/54326 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Method for handling microparticles in such a manner, that at least two treatment steps are performed for microparticles in the same vessel without moving the particles to another vessel. There are organs in the device for changing the solution without having to move the microparticles to another vessel.

公开(公告)号：US20160051992A1

公开(公告)日：2016-02-25

申请号：US14928096

申请日：2015-10-30

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： PRASANNA THWAR ,  VAMSEE K. PAMULA ,  ARJUN SUDARSAN ,  RAMAKRISHNA SISTA

IPC: B03C5/00 ,  B03C5/02 ,  B03C1/01

CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

公开(公告)号：US20160046926A1

公开(公告)日：2016-02-18

申请号：US14925157

申请日：2015-10-28

Applicant: Biological Dynamics, Inc.

Inventor： Rajaram KRISHNAN ,  David CHARLOT ,  Eugene TU ,  James MCCANNA ,  Lucas KUMOSA ,  Paul SWANSON ,  Robert TURNER ,  Kai YANG ,  Irina DOBROVOLSKAYA ,  David LIU

IPC: C12N15/10 ,  B03C7/02 ,  B03C5/02 ,  C12Q1/68 ,  B01D57/02

CPC classification number: B01L3/502715 ,  B01D57/02 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2400/0424 ,  B03C5/005 ,  B03C5/026 ,  B03C7/023 ,  B03C2201/26 ,  C12N15/1003 ,  C12N15/101 ,  C12Q1/6806 ,  G01N27/447 ,  G01N27/44704 ,  G01S5/163 ,  G01S11/12 ,  G06F3/0346

Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.

公开(公告)号：US09259732B2

公开(公告)日：2016-02-16

申请号：US14500584

申请日：2014-09-29

Applicant: GEN-PROBE INCORPORATED

Inventor： Christopher B. Davis ,  Norbert D. Hagen ,  James T. Tuggle

IPC: B03C1/12 ,  B01L3/00 ,  G01N35/00 ,  B03C1/033 ,  B03C1/28 ,  B65B3/04 ,  B03C1/14 ,  B01L9/00 ,  B25J11/00

CPC classification number: B01L3/52 ,  B01L3/561 ,  B01L9/52 ,  B01L2300/0609 ,  B01L2300/12 ,  B03C1/0332 ,  B03C1/12 ,  B03C1/14 ,  B03C1/28 ,  B03C1/286 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  B25J11/00 ,  B65B3/04 ,  C12N15/1013 ,  G01N35/0098 ,  Y10T29/49815

Abstract: A system for separating an analyte from other components of a sample contained in a receptacle, where the system includes a receptacle holding station and a magnetic separation station. The receptacle holding station is configured to receive and hold a receptacle and includes one or more magnets positioned to apply a magnetic field to the contents of the receptacle. When present in the receptacle holding station, a receptacle remains stationary relative to the one or more magnets. The magnetic separation station includes one or more magnets and is constructed and arranged to perform a magnetic separation procedure on the contents of a receptacle transported from the receptacle holding station to the magnetic separation station by an automated receptacle transport. The magnetic separation procedure includes isolating an analyte immobilized on a magnetically-responsive solid support within the receptacle and removing other components of the sample from the receptacle. The magnetic separation station is configured to provide relative movement between the receptacle and the one or more magnets after the receptacle is transported to the magnetic separation station.

Abstract translation: 用于将分析物与包含在容器中的样品的其它组分分离的系统，其中所述系统包括容器保持站和磁分离站。 容器保持站被配置为容纳和保持插座，并且包括一个或多个定位成将磁场施加到容器的内容物上的磁体。 当存在于容器保持站中时，容器相对于一个或多个磁体保持静止。 磁分离站包括一个或多个磁体，并被构造和布置成对通过自动插座传送从容器保持站传送到磁分离站的容器的内容物执行磁分离程序。 磁分离程序包括将固定在磁反应性固体支持物上的分析物分离在容器内并从容器中除去样品的其它组分。 磁性分离台构造成在容器被传送到磁分离站之后，在容器和一个或多个磁体之间提供相对运动。

公开(公告)号：US20150336096A1

公开(公告)日：2015-11-26

申请号：US14410985

申请日：2013-06-25

Applicant: The General Hospital Corporation

Inventor： Kyle C. Smith ,  Ramin Haghgooie ,  Thomas Alan Barber ,  Ismail Emre Ozkumur ,  Ravi Kapur ,  Mehmet Toner

IPC: B01L3/00 ,  G01N33/543 ,  G01N1/40

CPC classification number: B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/06 ,  B01L2300/12 ,  B01L2400/043 ,  B03C1/0332 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N1/4077 ,  G01N33/48735 ,  G01N33/54333

Abstract: This disclosure describes microfluidic devices that include one or more magnets, each magnet being operable to emit a magnetic field; and a magnetizable layer adjacent to the one or more magnets, in which the magnetizable layer is configured to induce a gradient in the magnetic field of at least one of the magnets. For example, the gradient can be at least 103 T/m at a position that is at least 20 μm away from a surface of the magnetizable layer. The magnetizable layer includes a first high magnetic permeability material and a low magnetic permeability material arranged adjacent to the high magnetic permeability material. The devices also include a microfluidic channel arranged on a surface of the magnetizable layer, wherein a central longitudinal axis of the microfluidic channel is arranged at an angle to or laterally offset from an interface between the high magnetic permeability material and the low magnetic permeability material.

Abstract translation: 本公开描述了包括一个或多个磁体的微流体装置，每个磁体可操作以发射磁场; 以及与所述一个或多个磁体相邻的可磁化层，其中所述可磁化层被配置为在至少一个所述磁体的磁场中引起梯度。 例如，在距可磁化层的表面至少20μm的位置处，梯度可以至少为103T / m。 可磁化层包括第一高磁导率材料和邻近高磁导率材料布置的低磁导率材料。 所述装置还包括布置在可磁化层的表面上的微流体通道，其中微流体通道的中心纵向轴线与高磁导率材料和低磁导率材料之间的界面成角度或横向偏移。

公开(公告)号：US09168527B2

公开(公告)日：2015-10-27

申请号：US13580343

申请日：2011-02-22

Applicant: Nathaniel D. Robinson ,  Per Erlandsson

Inventor： Nathaniel D. Robinson ,  Per Erlandsson

IPC: G01N27/453 ,  B01L3/00 ,  B03C5/02 ,  F04B19/00 ,  G01N27/447

CPC classification number: B01L3/50273 ,  B01L3/502707 ,  B01L2300/0816 ,  B01L2300/161 ,  B01L2400/0418 ,  B01L2400/0424 ,  B03C5/02 ,  B03C2201/26 ,  F04B19/006 ,  G01N27/44791 ,  Y10T137/0391 ,  Y10T137/206

Abstract: An electrokinetic fluidic system (100, 100′, 100″) for controlling liquid flow in e.g. a lab-on-a-chip system (200) comprising a first and a second electrode (10, 10′) said first and second electrode comprising a polymer based or oxide based conductive, electrochemically active electrode material, said electrode material being adapted to be subjected to an electrochemical reaction when in use in said electrokinetic fluid system (100).

Abstract translation: 用于控制例如液体流动的电动流体系统（100,100'，100“）。 包括第一和第二电极（10,10'）的第一和第二电极实验室（200），所述第一和第二电极包括聚合物基或氧化物导电的电化学活性电极材料，所述电极材料适于 当在所述电动流体系统（100）中使用时，其进行电化学反应。

公开(公告)号：US20150283553A1

公开(公告)日：2015-10-08

申请号：US14680819

申请日：2015-04-07

Applicant: Biological Dynamics, Inc.

Inventor： David Charlot ,  Juan Pablo HINESTROSA SALAZAR ,  Irina V. DOBROVOLSKAYA ,  Kai Yang ,  Paul SWANSON ,  Rajaram Krishnan

IPC: B03C5/00 ,  B03C5/02

CPC classification number: B03C5/005 ,  B03C5/026 ,  B03C2201/26

Abstract: The present invention includes methods, devices and systems for isolating nanoparticulates, including nucleic acids, from biological samples. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity isolation of biological components from complex fluids such as blood or environmental samples.

Abstract translation: 本发明包括从生物样品中分离纳米颗粒（包括核酸）的方法，装置和系统。 在各个方面，方法，装置和系统可以允许需要最少量的材料和/或导致生物组分与复杂流体例如血液或环境样品的高纯度分离的快速程序。

公开(公告)号：US20150233954A1

公开(公告)日：2015-08-20

申请号：US14706353

申请日：2015-05-07

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Shao-Hsing YEH ,  Yu-Ying LIN ,  Chi-Han CHIOU ,  Liang-Ju CHIEN

IPC: G01N35/00 ,  G01N1/28

CPC classification number: G01N35/0098 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2300/087 ,  B01L2400/043 ,  B03C1/01 ,  B03C1/0335 ,  B03C1/035 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N1/28 ,  Y10T137/0391 ,  Y10T137/206 ,  Y10T137/2191 ,  Y10T137/2224

Abstract: A method for manipulating a droplet by a droplet manipulating device including a flow channel, a first magnetic field generator, and a second magnetic field generator is provided. The first magnetic field generator includes two first magnetic field modules and are at two sides of the flow channel. The second magnetic field generator is between the two first magnetic field modules and includes multiple second magnetic field coils. The droplet is provided in the flow channel and includes a magnetic particle. A first magnetic field is produced on the flow channel by the first magnetic field modules, so the magnetic particle in the droplet has the direction of magnetic field corresponding to the first magnetic field. A second magnetic field is produced on the flow channel by the second magnetic field coils, for driving the magnetic particle in the droplet to be in motion in the flow channel.

Abstract translation: 提供了一种通过包括流动通道的液滴操纵装置来操纵液滴的方法，第一磁场发生器和第二磁场发生器。 第一磁场发生器包括两个第一磁场模块，并且位于流动通道的两侧。 第二磁场发生器位于两个第一磁场模块之间，并且包括多个第二磁场线圈。 液滴设置在流路中并包括磁性颗粒。 通过第一磁场模块在流动通道上产生第一磁场，因此液滴中的磁性颗粒具有对应于第一磁场的磁场方向。 通过第二磁场线圈在流路上产生第二磁场，用于驱动液滴中的磁性颗粒在流动通道中运动。