公开(公告)号：US09353363B2

公开(公告)日：2016-05-31

申请号：US13884773

申请日：2012-01-26

Applicant: Lene Lange ,  Peter K. Busk

Inventor： Lene Lange ,  Peter K. Busk

IPC: C12N9/42 ,  C12P19/14 ,  C12P21/00

CPC classification number: C12N9/2437 ,  C12N9/2445 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12P21/00 ,  C12P2201/00 ,  C12Y302/01004 ,  C12Y302/01091 ,  Y02E50/16

Abstract: The present invention relates to isolated polypeptides having cellulolytic activity or hemicellulolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 本发明涉及具有纤维素分解活性或半纤维素分解活性的分离多肽和编码多肽的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：US09353344B2

公开(公告)日：2016-05-31

申请号：US14887153

申请日：2015-10-19

Applicant: XYLECO, INC.

Inventor： Marshall Medoff ,  Thomas Craig Masterman

IPC: C08H8/00 ,  C12M1/42 ,  C10L1/02 ,  C12P7/10 ,  C12P7/40 ,  C12P7/62 ,  C12P19/14 ,  C12P7/02 ,  C12M1/00 ,  C12M1/26 ,  C12M1/34

CPC classification number: C07G1/00 ,  C08B37/0003 ,  C08H8/00 ,  C10G2300/1014 ,  C10L1/02 ,  C10L1/026 ,  C12M21/12 ,  C12M29/00 ,  C12M33/00 ,  C12M35/02 ,  C12M41/00 ,  C12M41/32 ,  C12M41/36 ,  C12M45/02 ,  C12M45/06 ,  C12M45/07 ,  C12M45/09 ,  C12P7/02 ,  C12P7/10 ,  C12P7/40 ,  C12P7/54 ,  C12P7/62 ,  C12P19/04 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  C13K1/02 ,  Y02E50/13 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/30 ,  Y02P30/20 ,  Y02T50/678

Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material.

公开(公告)号：US09352294B1

公开(公告)日：2016-05-31

申请号：US14853764

申请日：2015-09-14

Applicant: XYLECO, INC.

Inventor： Marshall Medoff

IPC: C10G65/02 ,  B01J19/08 ,  C07G1/00 ,  C10G11/00 ,  C10G47/00 ,  C10G35/04

CPC classification number: B01J19/08 ,  B01J19/081 ,  B01J19/085 ,  B01J2219/08 ,  B01J2219/0879 ,  C08B1/00 ,  C10G3/00 ,  C10G11/00 ,  C10G15/10 ,  C10G47/00 ,  C10G2300/1011 ,  C10L1/02 ,  C10L1/04 ,  C10L2200/0469 ,  C10L2270/02 ,  C10L2270/04 ,  C10L2290/02 ,  C10L2290/06 ,  C10L2290/26 ,  C10L2290/34 ,  C10L2290/36 ,  C10L2290/543 ,  C12P5/00 ,  C12P5/02 ,  C12P5/023 ,  C12P7/02 ,  C12P7/04 ,  C12P7/10 ,  C12P7/14 ,  C12P7/16 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  H01M8/1007 ,  H01M8/1013 ,  H01M8/16 ,  H01M8/22 ,  Y02E50/16 ,  Y02E50/343 ,  Y02P30/20 ,  Y02T50/678

Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.

公开(公告)号：US09347074B1

公开(公告)日：2016-05-24

申请号：US13506249

申请日：2012-04-06

Applicant: Dennis Anthony Burke

Inventor： Dennis Anthony Burke

IPC: C12Q1/00 ,  C12P7/00 ,  C12P7/06 ,  C12P7/08 ,  C12P7/10 ,  C08H8/00

CPC classification number: C12P7/06 ,  C08H8/00 ,  C12P5/023 ,  C12P7/00 ,  C12P7/08 ,  C12P7/10 ,  C12P2201/00 ,  C12Q1/00 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343 ,  Y02P20/129

Abstract: A simple, inexpensive, and benign process to pretreat lignocellulose biomass for the economical production of biofuel and extraction of organic chemicals. Lignocellulose solids are mixed or blended with ammonium bicarbonate/carbonate and heated within a pressure reactor. At elevated temperature (e.g., >35° C.), the ammonium bicarbonate/carbonate dissociates into ammonia and carbon dioxide gases and water vapor, thereby causing a rise in pressure within the pressure reactor. Rapid release of the gases from the pressure reactor then ruptures biomass cell wall structures, which facilitates conversion of the cellulose and hemicellulose in the pretreated biomass to sugars that are fermentable into ethanol or other liquid fuels. Optionally, ammonia bicarbonate/carbonate can be reconstituted by cooling and precipitating the carbon dioxide and ammonia gases released from the pressure reactor for further use in the pretreatment process or sequestered as an end product.

Abstract translation: 一种简单，便宜和良性的预处理木质纤维素生物质以经济生产生物燃料和提取有机化学品的方法。 将木质素纤维素固体与碳酸氢铵/碳酸盐混合或混合并在压力反应器内加热。 在升高的温度（例如> 35℃）下，碳酸氢铵/碳酸盐解离成氨和二氧化碳气体和水蒸汽，从而导致压力反应器内的压力升高。 来自压力反应器的气体的快速释放然后使生物质细胞壁结构破裂，这有助于将预处理的生物质中的纤维素和半纤维素转化为可发酵成乙醇或其它液体燃料的糖。 任选地，氨碳酸氢盐/碳酸盐可以通过冷却和沉淀从压力反应器中释放的二氧化碳和氨气进行重构，以便在预处理过程中进一步使用或螯合作为最终产物。

公开(公告)号：US20160115503A1

公开(公告)日：2016-04-28

申请号：US14893577

申请日：2014-05-27

Applicant: VALICOR, INC.

Inventor： James R. Bleyer ,  Thomas Czartoski

IPC: C12P7/06 ,  C12N1/00

CPC classification number: C12P7/06 ,  C12N1/005 ,  C12P2201/00 ,  Y02E50/17

Abstract: A method of improving fermentation by increasing the bioavailability of components in stillage, using all or a portion of the hydrothermally treated stillage as a component of a media, and using the media for a process such as fermentation or biomass production. The metabolites and biomass recovered from the method above. Media including hydrothermally treating stillage obtained by heating the stillage to a temperature of 190° F. to 300° F. A method of improving fermentation by removing solids in stillage, hydrothermally treating the stillage by heating the stillage to a temperature of 190° F. to 300° F., using all or a portion of the stillage as a component of a media, and using the media for a process chosen from the group consisting of fermentation and biomass production.

Abstract translation: 通过使用全部或部分水热处理的酒糟作为培养基的组分，以及使用培养基进行诸如发酵或生物质生产的过程，通过提高釜中组分的生物利用度来改进发酵的方法。 从上述方法回收的代谢物和生物量。 介质包括水热处理通过将釜渣加热至190°F至300°F的釜渣而获得的淀粉。通过除去釜中的固体来改善发酵的方法，通过将釜渣加热至190°F进行水热处理。 至300°F，使用全部或部分的釜渣作为培养基的组分，并使用培养基用于选自发酵和生物质生产的方法。

公开(公告)号：US09296830B2

公开(公告)日：2016-03-29

申请号：US13817869

申请日：2011-09-29

Applicant: Piero Ottonello ,  Simone Ferrero ,  Paolo Torre ,  Francesco Cherchi ,  Danilo Defaveri ,  Luis Oriani

Inventor： Piero Ottonello ,  Simone Ferrero ,  Paolo Torre ,  Francesco Cherchi ,  Danilo Defaveri ,  Luis Oriani

IPC: D21B1/36 ,  C08B37/00 ,  C13K1/02 ,  C13K13/00 ,  C12P19/02 ,  D21B1/02 ,  D21B1/12 ,  D21C1/02 ,  C08H8/00

CPC classification number: C08B37/0057 ,  C08H8/00 ,  C12P19/02 ,  C12P2201/00 ,  C13K1/02 ,  C13K13/002 ,  C13K13/007 ,  D21B1/02 ,  D21B1/12 ,  D21B1/36 ,  D21C1/02

Abstract: This specification discloses an improved method for conducting the removal of C5 xylan based sugars from biomass. The improved method involves a series of soakings and washings of the biomass as opposed to conducting one soaking and washing step.

Abstract translation: 本说明书公开了一种用于从生物质中去除C5木聚糖基糖的改进方法。 与进行一次浸泡和洗涤步骤相反，改进的方法涉及生物质的一系列浸泡和洗涤。

公开(公告)号：US20160076014A1

公开(公告)日：2016-03-17

申请号：US14016461

申请日：2013-09-03

Applicant: Xyleco, Inc.

Inventor： Marshall MEDOFF ,  Thomas Craig MASTERMAN ,  James LYNCH

IPC: C12N9/42 ,  C12P7/16 ,  C12P19/02 ,  C12P19/12 ,  C12P19/04 ,  C12P5/02 ,  C12P7/62 ,  C12P7/28 ,  C12P7/42 ,  C12P7/24 ,  C12P7/04 ,  C12P7/56 ,  C12P7/48 ,  C12P7/52 ,  C12P7/46 ,  C12P7/40 ,  C12P7/10

CPC classification number: C12P19/14 ,  C12N9/2434 ,  C12N9/2437 ,  C12P5/02 ,  C12P5/023 ,  C12P7/04 ,  C12P7/10 ,  C12P7/16 ,  C12P7/24 ,  C12P7/28 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/52 ,  C12P7/56 ,  C12P7/62 ,  C12P19/02 ,  C12P19/04 ,  C12P19/12 ,  C12P2201/00 ,  C12Y302/01004 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

Abstract: Provided herein are methods for processing biomass materials that are disposed in one or more structures or carriers, e.g., a bag, a shell, a net, a membrane, a mesh or any combination of these. Containing the material in this manner allows it to be readily added or removed at any point and in any sequence during processing.

Abstract translation: 本文提供了处理生物质材料的方法，所述生物质材料被布置在一个或多个结构或载体中，例如袋，壳，网，膜，网或这些的任何组合。 以这种方式包含材料允许其在加工期间以任何点和任何顺序容易地添加或除去。

公开(公告)号：US09284614B2

公开(公告)日：2016-03-15

申请号：US14006482

申请日：2012-03-08

Applicant: Ichiro Fujita

Inventor： Ichiro Fujita

IPC: C13K1/02 ,  B09B3/00 ,  C12P19/02

CPC classification number: C13K1/02 ,  B09B3/00 ,  C12P19/02 ,  C12P2201/00

Abstract: The invention relates to: a pre-treatment method for plant biomass hydrolysis reaction raw materials characterized in comprising a process for mixing a solid catalyst and solid substrate beforehand and grinding same simultaneously (grinding process); a plant biomass hydrolysis reaction raw material pre-treated by said pre-treatment method; and a plant biomass saccharification method comprising a process for hydrolyzing said hydrolysis reaction raw material. The invention provides an efficient and practical pre-treatment method for plant biomass hydrolysis reaction raw materials that can improve the saccharification yield and saccharide concentration of plant biomass hydrolysis reactions, a plant biomass hydrolysis reaction raw material obtained therefrom, and a plant biomass saccharification method.

Abstract translation: 本发明涉及：用于植物生物质水解反应原料的预处理方法，其特征在于包括预先将固体催化剂和固体基质混合并同时研磨的方法（研磨过程）; 通过所述预处理方法预处理的植物生物质水解反应原料; 以及包括水解所述水解反应原料的方法的植物生物质糖化方法。 本发明提供了一种能够提高植物生物质水解反应的糖化产率和糖浓度的植物生物质水解反应原料的有效实用的预处理方法，由其获得的植物生物量水解反应原料和植物生物质糖化方法。

公开(公告)号：US20160069020A1

公开(公告)日：2016-03-10

申请号：US14940992

申请日：2015-11-13

Applicant: GREENFIELD SPECIALTY ALCOHOLS INC.

Inventor： Frank A. DOTTORI ,  Robert Ashley Cooper BENSON ,  Régis-Olivier BENECH

IPC: D21C1/02

CPC classification number: D21C1/02 ,  C08B37/0057 ,  C08H8/00 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C13K1/02 ,  C13K13/002 ,  D21C11/0007 ,  D21H11/12

Abstract: A process is defined for the continuous steam pretreatment and fractionation of bagasse to produce a concentrated cellulose solid stream that is sensitive to enzymatic hydrolysis. Valuable chemicals are recovered by fractionating the liquid and vapor stream composed of hydrolysis and degradation products of the hemicellulose. Cellulosic derived glucose is produced for fermentation to biofuels. A hemicellulose concentrate is recovered that can be converted to value added products including ethanol.

Abstract translation: 定义了一种用于连续蒸汽预处理和甘蔗渣分级以产生对酶水解敏感的浓缩纤维素固体物流的方法。 通过分离由半纤维素的水解和降解产物组成的液体和蒸汽流来回收有价值的化学品。 生产用于发酵的生物燃料的纤维素衍生的葡萄糖。 回收可以转化成包括乙醇在内的增值产品的半纤维素浓缩物。

公开(公告)号：US20160068828A1

公开(公告)日：2016-03-10

申请号：US14849276

申请日：2015-09-09

Applicant: PURDUE RESEARCH FOUNDATION

Inventor： Michael Ralph Ladisch ,  Eduardo de Aquino Ximenes ,  Thomas Richard Kreke ,  Alberto Colli Badino, JR. ,  Fernanda Marisa da Cunha ,  Cristiane Sanchez Farinas

IPC: C12N9/42

CPC classification number: C12N9/2437 ,  C12N1/14 ,  C12N1/20 ,  C12N1/22 ,  C12N9/244 ,  C12P21/02 ,  C12P2201/00 ,  C12Y302/01004

Abstract: Methods for enhancing production of a cellulose component are disclosed herein.

Abstract translation: 本文公开了增强纤维素成分生产的方法。