公开(公告)号：US20250116852A1

公开(公告)日：2025-04-10

申请号：US18906687

申请日：2024-10-04

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Hongli Ni ,  Yuhao Yuan

IPC: G02B21/08 ,  G01N21/63

Abstract: A short-wave infrared photothermal (SWIP) microscopy system and method for vibrational imaging of a sample generates shortwave infrared excitation light probe light. The excitation light and the probe light are combined to generate a combined beam, which is focused to generate a focused combined beam, which is directed onto the sample to obtain a SWIP signal generated by absorption-induced thermo-optic selective heating of the sample. The SWIP signal is collected through an aperture in a condenser and detected.

公开(公告)号：US20250115593A1

公开(公告)日：2025-04-10

申请号：US18904487

申请日：2024-10-02

Applicant: TRUSTEES OF BOSTON UNIVERSITY ,  University of Massachusetts

Inventor： John A. PORCO, Jr. ,  Zihao WANG ,  Lauren E. BROWN ,  Stanley I. Goldstein ,  Sunll K. MALONIA ,  Ritesh P. THAKARE ,  Alok K. MISHRA ,  Michael R. Green

IPC: C07D413/12 ,  A61K31/343 ,  A61K31/397 ,  A61K31/4025 ,  A61K31/4184 ,  A61K31/4525 ,  A61K31/5377 ,  A61P35/00 ,  C07D307/93 ,  C07D405/04 ,  C07D405/10 ,  C07D405/12

Abstract: The disclosure provides rocaglate acyl sulfamides (Roc ASFs), compositions and kits comprising same, and methods for their use.

公开(公告)号：US20250090597A1

公开(公告)日：2025-03-20

申请号：US18792994

申请日：2024-08-02

Applicant: TRUSTEES OF BOSTON UNIVERSITY

Inventor： Liang MA ,  Darrell Kotton ,  Finn Hawkins

IPC: A61K35/42 ,  A61P11/00 ,  C12N5/071

Abstract: The technology described herein is directed to engraftment methods for airway basal cells into the respiratory tract of a subject, following removal of epithelial cells of the respiratory tract. Also described herein are methods of treating a respiratory tract disease or a respiratory tract injury.

公开(公告)号：US20250059243A1

公开(公告)日：2025-02-20

申请号：US18756596

申请日：2024-06-27

Applicant: TRUSTEES OF BOSTON UNIVERSITY

Inventor： Ahmad S. KHALIL ,  Kok Ann GAN ,  Hanrong YE ,  Thea Samuelle ORNSTEIN

IPC: C07K14/47 ,  C12N15/63

Abstract: The technology described herein is directed to regulated synthetic gene expression systems. In one aspect described herein are synthetic transcription factors (synTFs) comprising a DNA binding domain, a transcriptional activator domain, a transcriptional effector domain (TED), and optionally a regulator protein. In other aspects described herein are gene expression systems comprising said synTFs and methods of treating diseases and disorders using said synTFs.

公开(公告)号：US20250027114A1

公开(公告)日：2025-01-23

申请号：US18906028

申请日：2024-10-03

Applicant: The Broad Institute, Inc. ,  President and Fellows of Harvard College ,  Trustees of Boston University

Inventor： David R. Liu ,  Tony P. Huang ,  Zachary J. Heins ,  Ahmad S. Khalil

IPC: C12N15/90 ,  C12N9/22 ,  C12N9/78 ,  C12N15/11

Abstract: The present disclosure provides Cas9 variants, and base editors comprising these variants, that recognize non-canonical protospacer adjacent motifs (PAMs) and have less restrictive PAM requirements for editing. The present disclosure provides Cas9 protein variants comprising one or more amino acid substitutions relative to wild-type Nme2Cas9. Fusion proteins comprising the Cas protein variants described herein are also provided by the present disclosure. Further provided herein are methods for editing a target nucleic acid using the Cas variants and fusion proteins provided herein. The present disclosure also provides guide RNAs, complexes, polynucleotides, cells, kits, and pharmaceutical compositions. Further described herein are phage-assisted continuous evolution (PACE) systems, vectors, methods, and devices.

公开(公告)号：US20250012557A1

公开(公告)日：2025-01-09

申请号：US18761623

申请日：2024-07-02

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Haonan Zong ,  Celalettin Yurdakul ,  M. Selim Ünlü

IPC: G01B9/02091

Abstract: A wide-field bond-selective optical coherence tomography (OCT) system and method for imaging a sample includes generating infrared light and directing the infrared light onto the sample to selectively heat the sample. Probe light is also directed onto the sample. A first actuator provides sample depth scanning with respect to a first objective in a reference arm of the system, and a second actuator provides sample depth scanning with respect to a second objective in a sample arm of the system. A detection system receives scattered probe light reflected from the sample. A change in the received probe light from the sample that is indicative of absorption of infrared light.

公开(公告)号：US12173320B2

公开(公告)日：2024-12-24

申请号：US17407349

申请日：2021-08-20

Applicant: BOSTON MEDICAL CENTER CORPORATION ,  TRUSTEES OF BOSTON UNIVERSITY

Inventor： George J. Murphy ,  David H. Sherr ,  Sarah S. Rozelle ,  Brenden W. Smith

IPC: C12N5/0789 ,  A61K31/404 ,  A61K31/655 ,  A61K35/18 ,  A61K35/19 ,  A61K45/06 ,  C12N5/078 ,  G01N33/50 ,  G01N33/80 ,  G01N33/86 ,  A61K35/12

Abstract: This disclosure provides methods of making a megakaryocyte-erythroid progenitor cell (MEP), comprising differentiating a MEP precursor cell into a MEP in culture in the presence of an aryl hydrocarbon receptor (AhR) modulator. In some embodiments the AhR modulator is an AhR antagonist. In some embodiments the AhR modulator is an AhR agonist. In some embodiments the methods comprise culturing MEP precursor cells in the presence of an AHR antagonist and then culturing MEP precursor cells in the presence of an AHR agonist. In some embodiments the stem cell is a pluripotent stem cell. In some embodiments the MEP co-expresses CD41 and CD235. In some embodiments the number of MEPs produced in the culture increases exponentially. Methods of making a red blood cell (RBC) by culturing a MEP in the presence of an AhR modulator are also provided. Methods of making a megakaryocyte and/or a platelet, comprising culturing a MEP in the presence of an AhR modulator are also provided. In some embodiments the AhR modulator is an AhR antagonist. This disclosure also provides compositions comprising at least 1 million MEPs per ml and compositions in which at least 50% of the cells are MEPs, among other things.

公开(公告)号：US20240421971A1

公开(公告)日：2024-12-19

申请号：US18742330

申请日：2024-06-13

Applicant: Trustees of Boston University

Inventor： Rashmi S. Agrawal ,  Ajay J. Joshi

IPC: H04L9/00

Abstract: An FPGA-based accelerator for bootstrappable fully homomorphic encryption (FHE) employs (1) acceleration of scalar arithmetic operations using a multi-word approach for efficient utilization of standard-width components (multipliers/adders) on custom-width operands; (2) a performant, shift-based modular reduction technique that avoids the need for expensive multipliers; (3) an improved datapath for an expensive Key Switch operation; and (4) an efficient organization of on-chip memory for storing custom-width operands and supplying them at high bandwidth to computation units.

公开(公告)号：US12147022B2

公开(公告)日：2024-11-19

申请号：US18205152

申请日：2023-06-02

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Celalettin Yurdakul ,  Haonan Zong ,  M. Selim Ünlü

IPC: G02B21/16 ,  G02B21/02 ,  G02B21/36

Abstract: Microscopic analysis of a sample includes a system using dark-field illumination. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. A visible light source generates a light illuminating the sample on a substrate and creating a scattered field and a reflected field along a collection path of the system. A pupil mask is positioned along the collection path to block the reflected field while allowing the scattered field to pass therethrough. A camera is positioned at an end of the collection path to collect the scattered field and generate a dark-field image of the sample.

公开(公告)号：US12140775B2

公开(公告)日：2024-11-12

申请号：US18226483

申请日：2023-07-26

Applicant: Trustees of Boston University

Inventor： M. Selim Ünlü ,  Mete Aslan ,  Iris Celebi ,  Celalettin Yurdakul ,  Allison Marie Marn

IPC: G02B27/09 ,  G02B21/08 ,  G02B27/48

Abstract: An exemplary illumination source is provided. The illumination source includes a light integrating device for coupling to at least one optical structure at an output port. The light integrating device includes at least one input port. At least one light source produces at least one optical illumination coupled to the at least one input port. A light adjusting tool controls the optical illumination emitted by the light integrating device. The light adjusting tool controls uniformity of light emitted by the light integrating device by modifying at least one internal surface of the light integrating device.