公开(公告)号：US07662415B2

公开(公告)日：2010-02-16

申请号：US11821489

申请日：2007-06-22

Applicant: Michael Bauer ,  Michael Richard Bedford ,  Derrick Allen Pulliam

Inventor： Michael Bauer ,  Michael Richard Bedford ,  Derrick Allen Pulliam

IPC: A23K3/00 ,  C12N9/24 ,  C12N9/00 ,  C12P21/06 ,  A61K38/43 ,  A61K38/54 ,  C07H21/04

CPC classification number: C12N9/2482 ,  A23K20/189 ,  A23K20/20 ,  A23K40/20 ,  A23K40/25 ,  A23K50/10 ,  A23K50/20 ,  A23K50/30 ,  A23K50/75 ,  A23K50/80 ,  A23L33/195 ,  C07K2319/02 ,  C12N9/248 ,  C12Y302/01008 ,  Y02A40/818

Abstract: The present invention relates to codon-optimized xylanase coding sequences and the expression of xylanases in microbes and yeast. The invention further relates to using multiple copies of the xylanase expression construct for high levels of protein expression. The invention also relates to the use of xylanases as feed or food additives. The invention also relates to methods of expression of enzymes to increase thermotolerance by expressing them in organisms that glycosylate proteins compared to expression that the same enzyme without the glycosylation. Further, the invention relates to methods of preparing feed, enzyme feed additives, and methods of reducing the feed conversion ration or increasing weight gain of animals.

Abstract translation: 本发明涉及密码子优化的木聚糖酶编码序列和微生物和酵母中木聚糖酶的表达。 本发明还涉及使用木聚糖酶表达构建体的多个拷贝用于高水平的蛋白质表达。 本发明还涉及木聚糖酶作为饲料或食品添加剂的用途。 本发明还涉及通过在与没有糖基化的相同酶的表达相比糖蛋白的生物体中表达它们来增加耐热性来增加耐热性的方法。 此外，本发明涉及制备饲料，酶饲料添加剂的方法以及减少饲料转化率或增加动物体重增加的方法。

公开(公告)号：US20080187627A1

公开(公告)日：2008-08-07

申请号：US11821489

申请日：2007-06-22

Applicant: Michael Bauer ,  Michael Richard Bedford ,  Derrick Allen Pulliam

Inventor： Michael Bauer ,  Michael Richard Bedford ,  Derrick Allen Pulliam

IPC: A23K1/00 ,  C12N9/42 ,  A23L1/48 ,  A23L1/10 ,  A23L1/20

CPC classification number: C12N9/2482 ,  A23K20/189 ,  A23K20/20 ,  A23K40/20 ,  A23K40/25 ,  A23K50/10 ,  A23K50/20 ,  A23K50/30 ,  A23K50/75 ,  A23K50/80 ,  A23L33/195 ,  C07K2319/02 ,  C12N9/248 ,  C12Y302/01008 ,  Y02A40/818

Abstract: The present invention relates to codon-optimized xylanase coding sequences and the expression of xylanases in microbes and yeast. The invention further relates to using multiple copies of the xylanase expression construct for high levels of protein expression. The invention also relates to the use of xylanases as feed or food additives. The invention also relates to methods of expression of enzymes to increase thermotolerance by expressing them in organisms that glycosylate proteins compared to expression that the same enzyme without the glycosylation. Further, the invention relates to methods of preparing feed, enzyme feed additives, and methods of reducing the feed conversion ration or increasing weight gain of animals.

Abstract translation: 本发明涉及密码子优化的木聚糖酶编码序列和微生物和酵母中木聚糖酶的表达。 本发明还涉及使用木聚糖酶表达构建体的多个拷贝用于高水平的蛋白质表达。 本发明还涉及木聚糖酶作为饲料或食品添加剂的用途。 本发明还涉及通过在与没有糖基化的相同酶的表达相比糖蛋白的生物体中表达它们来增加耐热性来增加耐热性的方法。 此外，本发明涉及制备饲料，酶饲料添加剂的方法以及减少饲料转化率或增加动物体重增加的方法。

公开(公告)号：US09207117B2

公开(公告)日：2015-12-08

申请号：US13812323

申请日：2011-07-28

Applicant: Karl Booksh ,  Nicola Menegazzo ,  Yoon-Chang Kim ,  Derrick Allen ,  Lauren Kegel

Inventor： Karl Booksh ,  Nicola Menegazzo ,  Yoon-Chang Kim ,  Derrick Allen ,  Lauren Kegel

IPC: G02B5/20 ,  G01J3/42 ,  G01N21/35 ,  G01N21/552 ,  G01J3/02

CPC classification number: G01J3/0205 ,  G01J3/42 ,  G01N21/35 ,  G01N21/553 ,  G02B5/208

Abstract: Methods of measuring a sample characteristic and accessories for infrared (IR) spectrometers are provided. An accessory includes an input port and an output port having an optical path therebetween, a surface plasmon resonance (SPR) structure for contacting a sample, a mirror system, and an optical element for producing collimated light. The SPR structure produces internally reflected light responsive to broadband IR light, modified by a SPR between the SPR structure and the sample. The mirror system directs the broadband IR light from the input port to the SPR structure and directs the internally reflected light from the SPR structure to the output port, producing output light indicative of a characteristic of the sample associated with the SPR. The optical element is disposed along the optical path between the input port and the output port.

Abstract translation: 提供了测量红外（IR）光谱仪的样品特性和附件的方法。 一个附件包括一个输入端口和一个具有光路的输出端口，用于接触样品的表面等离子体共振（SPR）结构，镜子系统和用于产生准直光的光学元件。 SPR结构产生内部反射光，响应宽带IR光，由SPR结构和样品之间的SPR进行修改。 镜系统将宽带IR光从输入端口引导到SPR结构，并将内部反射光从SPR结构引导到输出端口，产生指示与SPR相关联的样本特征的输出光。 光学元件沿着输入端口和输出端口之间的光路布置。

公开(公告)号：US20130240734A1

公开(公告)日：2013-09-19

申请号：US13812323

申请日：2011-07-28

Applicant: Karl Booksh ,  Nicola Menegazzo ,  Yoon-Chang Kim ,  Laurel Kegel ,  Derrick Allen

Inventor： Karl Booksh ,  Nicola Menegazzo ,  Yoon-Chang Kim ,  Laurel Kegel ,  Derrick Allen

IPC: G01J3/02 ,  G02B5/20 ,  G01J3/42

CPC classification number: G01J3/0205 ,  G01J3/42 ,  G01N21/35 ,  G01N21/553 ,  G02B5/208

Abstract: Methods of measuring a sample characteristic and accessories for infrared (IR) spectrometers are provided. An accessory includes an input port and an output port having an optical path therebetween, a surface plasmon resonance (SPR) structure for contacting a sample, a mirror system, and an optical element for producing collimated light. The SPR structure produces internally reflected light responsive to broadband IR light, modified by a SPR between the SPR structure and the sample. The mirror system directs the broadband IR light from the input port to the SPR structure and directs the internally reflected light from the SPR structure to the output port, producing output light indicative of a characteristic of the sample associated with the SPR. The optical element is disposed along the optical path between the input port and the output port.

Abstract translation: 提供了测量红外（IR）光谱仪的样品特性和附件的方法。 一个附件包括一个输入端口和一个具有光路的输出端口，用于接触样品的表面等离子体共振（SPR）结构，镜子系统和用于产生准直光的光学元件。 SPR结构产生内部反射光，响应宽带IR光，由SPR结构和样品之间的SPR进行修改。 镜系统将宽带IR光从输入端口引导到SPR结构，并将内部反射光从SPR结构引导到输出端口，产生指示与SPR相关联的样本特征的输出光。 光学元件沿着输入端口和输出端口之间的光路布置。

公开(公告)号：US07291493B2

公开(公告)日：2007-11-06

申请号：US11018645

申请日：2004-12-20

Applicant: Michael Bauer ,  Michael Richard Bedford ,  Derrick Allen Pulliam

Inventor： Michael Bauer ,  Michael Richard Bedford ,  Derrick Allen Pulliam

IPC: C12N9/24 ,  C12N1/00 ,  C12Q1/68 ,  C12P21/06 ,  A23B7/00 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12N9/2482 ,  A23K20/189 ,  A23K20/20 ,  A23K40/20 ,  A23K40/25 ,  A23K50/10 ,  A23K50/20 ,  A23K50/30 ,  A23K50/75 ,  A23K50/80 ,  A23L33/195 ,  C07K2319/02 ,  C12N9/248 ,  C12Y302/01008 ,  Y02A40/818

Abstract: The present invention relates to codon-optimized xylanase coding sequences and the expression of xylanases in microbes and yeast. The invention further relates to using multiple copies of the xylanase expression construct for high levels of protein expression. The invention also relates to the use of xylanases as feed or food additives. The invention also relates to methods of expression of enzymes to increase thermotolerance by expressing them in organisms that glycosylate proteins compared to expression that the same enzyme without the glycosylation. Further, the invention relates to methods of preparing feed, enzyme feed additives, and methods of reducing the feed conversion ration or increasing weight gain of animals.

Abstract translation: 本发明涉及密码子优化的木聚糖酶编码序列和微生物和酵母中木聚糖酶的表达。 本发明还涉及使用木聚糖酶表达构建体的多个拷贝用于高水平的蛋白质表达。 本发明还涉及木聚糖酶作为饲料或食品添加剂的用途。 本发明还涉及通过在与没有糖基化的相同酶的表达相比糖蛋白的生物体中表达它们来增加耐热性来增加耐热性的方法。 此外，本发明涉及制备饲料，酶饲料添加剂的方法以及减少饲料转化率或增加动物体重增加的方法。