公开(公告)号：US20120178146A1

公开(公告)日：2012-07-12

申请号：US13262647

申请日：2010-03-26

Applicant: Marc Auffret ,  Francoise Fayolle-Guichard ,  Gerald Thouand ,  Charles W. Greer ,  Diane Labbe

Inventor： Marc Auffret ,  Francoise Fayolle-Guichard ,  Gerald Thouand ,  Charles W. Greer ,  Diane Labbe

IPC: C12N1/20 ,  C12S3/00

CPC classification number: C12N1/26 ,  C02F3/344 ,  C12P39/00 ,  C12R1/01

Abstract: This invention relates to new Rhodococcus wratislaviensis CNCM I-4088 bacteria or Rhodococcus aetherivorans CNCM I-4089 bacteria that can degrade multiple petroleum compounds in solution in aqueous effluents.The invention also relates to a process for treating aqueous effluents comprising a complex mixture of substances containing native hydrocarbons of gasolines and additives that are present in gasolines or diesel fuel, in which process said bacteria are grown under aerobic conditions in the presence of a growth substrate containing said mixture as a carbon source, and said mixture is at least partially degraded by the bacteria down to the final degradation products—carbon dioxide, water and biomass.

Abstract translation: 本发明涉及新颖的红球菌（Rhodococcus wratislaviensis）CNCM I-4088细菌或能够在含水废水溶液中降解多种石油化合物的红霉球菌（Cronococcus aetherivorans）CNCM I-4089细菌。 本发明还涉及一种处理含水流出物的方法，其包含含有汽油天然烃和汽油或柴油燃料中的添加剂的物质的复杂混合物，其中所述细菌在需氧条件下在生长底物存在下生长 含有所述混合物作为碳源，并且所述混合物至少部分地被细菌降解至最终降解产物 - 二氧化碳，水和生物质。

公开(公告)号：US20080210631A1

公开(公告)日：2008-09-04

申请号：US11996127

申请日：2006-07-18

Applicant: Nicolas Lopes Frreira ,  Diane Labbe ,  Helena Maciel ,  Francoise Fayolle-Guichard ,  Frederic Monot ,  Charles W. Greer

Inventor： Nicolas Lopes Frreira ,  Diane Labbe ,  Helena Maciel ,  Francoise Fayolle-Guichard ,  Frederic Monot ,  Charles W. Greer

IPC: C02F3/34 ,  C07K14/35 ,  C12N9/04 ,  C07H21/04 ,  C12N15/63 ,  C12N1/00 ,  C12N1/21 ,  C12Q1/68 ,  C12Q1/02 ,  C40B60/00

CPC classification number: C02F3/34 ,  C12N9/0006 ,  C12N9/0008

Abstract: The invention concerns an isolated or purified polypeptide having an activity in the MTBE degradation path, and/or at least one of the catabolic intermediates of MTBE, preferably selected from the group consisting of tert-butyl alcohol (TBA), 2-methyl 1,2-propanediol (2-M1, 2-PD), hydroxyisobutyraldehyde, hydroxyiso-butyric acid (HIBA), said polypeptide being selected from the group consisting of: a) a polypeptide comprising a sequence of amino acids selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 or SEQ ID NO:10; b) a polypeptide comprising a sequence of amino acids having at least 70% identity, preferably 75%, 80%, 90%, 95%, 98% or 99% with the sequence of amino acids of a polypeptide as defined in a); a polypeptide as defined in a) or b) whereof the sequence of amino acids comprises a substitution, deletion, insertion, addition or mutation of one or several amino acids over its entire length.

Abstract translation: 本发明涉及在MTBE降解途径中具有活性的分离或纯化的多肽，和/或MTBE的分解代谢中间体中的至少一种，优选选自叔丁醇（TBA），2-甲基1， 2-丙二醇（2-M1，2-PD），羟基异丁醛，羟基异丁酸（HIBA），所述多肽选自：a）包含选自下组的氨基酸序列的多肽 SEQ ID NO：2，SEQ ID NO：4，SEQ ID NO：6，SEQ ID NO：8或SEQ ID NO：10; b）包含与a）中所定义的多肽的氨基酸序列具有至少70％同一性，优选75％，80％，90％，95％，98％或99％的氨基酸序列的多肽; a）或b）中定义的多肽，其中氨基酸序列包括其整个长度上一个或几个氨基酸的取代，缺失，插入，添加或突变。

公开(公告)号：US07867741B2

公开(公告)日：2011-01-11

申请号：US11996127

申请日：2006-07-18

Applicant: Nicolas Lopes Ferreira ,  Diane Labbe ,  Héléna Maciel ,  Françoise Fayolle-Guichard ,  Frédéric Monot ,  Charles W. Greer

Inventor： Nicolas Lopes Ferreira ,  Diane Labbe ,  Héléna Maciel ,  Françoise Fayolle-Guichard ,  Frédéric Monot ,  Charles W. Greer

IPC: C12N9/02 ,  C12P21/06

CPC classification number: C02F3/34 ,  C12N9/0006 ,  C12N9/0008

Abstract: The invention concerns an isolated or purified polypeptide having an activity in the MTBE degradation path, and/or at least one of the catabolic intermediates of MTBE, preferably selected from the group consisting of tert-butyl alcohol (TBA), 2-methyl 1,2-propanediol (2-M1, 2-PD), hydroxyisobutyraldehyde, hydroxyiso-butyric acid (HIBA), said polypeptide being selected from the group consisting of: a) a polypeptide comprising a sequence of amino acids selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 or SEQ ID NO:10; b) a polypeptide comprising a sequence of amino acids having at least 70% identity, preferably 75%, 80%, 90%, 95%, 98% or 99% with the sequence of amino acids of a polypeptide as defined in a); a polypeptide as defined in a) or b) whereof the sequence of amino acids comprises a substitution, deletion, insertion, addition or mutation of one or several amino acids over its entire length.

Abstract translation: 本发明涉及在MTBE降解途径中具有活性的分离或纯化的多肽，和/或MTBE的分解代谢中间体中的至少一种，优选选自叔丁醇（TBA），2-甲基1， 2-丙二醇（2-M1，2-PD），羟基异丁醛，羟基异丁酸（HIBA），所述多肽选自：a）包含选自下组的氨基酸序列的多肽 SEQ ID NO：2，SEQ ID NO：4，SEQ ID NO：6，SEQ ID NO：8或SEQ ID NO：10; b）包含与a）中所定义的多肽的氨基酸序列具有至少70％同一性，优选75％，80％，90％，95％，98％或99％的氨基酸序列的多肽; a）或b）中定义的多肽，其中氨基酸序列包括其整个长度上一个或几个氨基酸的取代，缺失，插入，添加或突变。