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    METHOD FOR PRODUCING CELLULOLYTIC AND/OR HEMICELLULOLYTIC ENZYMES
    1.
    发明申请
    METHOD FOR PRODUCING CELLULOLYTIC AND/OR HEMICELLULOLYTIC ENZYMES 审中-公开
    生产纤维素和/或抗细胞纤维素酶的方法

    公开(公告)号：US20130210119A1

    公开(公告)日：2013-08-15

    申请号：US13809734

    申请日：2011-06-16

    Applicant: Fadhel Ben Chaabane ,  Frederic Monot

    Inventor： Fadhel Ben Chaabane ,  Frederic Monot

    IPC: C12N9/42

    CPC classification number: C12N9/2437 ,  C12N1/14 ,  C12N9/2434 ,  C12N9/248 ,  C12P21/02 ,  Y02E50/16

    Abstract: The process for the production of cellulolytic and/or hemicellulolytic enzymes by a cellulolytic and/or hemicellulolytic microorganism according to the present invention comprises at least one phase for growth in the presence of a source of carbon and at least one phase for production in the presence of an inducing substrate, in which said inducing substrate is a mixture comprising 40% to 65% by weight of glucose or cellulosic hydrolysates, 21% to 25% by weight of lactose and 10% to 39% by weight of xylose or a solution of a lignocellulosic hemicellulosic hydrolysate, the sum of these three constituents being equal to 100%.

    Abstract translation: 通过根据本发明的纤维素分解和/或半纤维素分解微生物生产纤维素分解和/或半纤维素分解酶的方法包括至少一个在碳源存在下生长的相和用于在存在下生产的至少一个相 的诱导底物，其中所述诱导底物是包含40重量％至65重量％的葡萄糖或纤维素水解产物，21重量％至25重量％的乳糖和10重量％至39重量％的木糖的混合物或 木质纤维素半纤维素水解物，这三种组分的总和等于100％。

    Method for treating bacterial effluents containing at least a Gordonia terrae CIP I-2194 ether
    2.
    发明授权
    Method for treating bacterial effluents containing at least a Gordonia terrae CIP I-2194 ether 有权
    用于处理含有至少一种Gordonia terrae CIP I-2194醚的细菌流出物的方法

    公开(公告)号：US07166457B1

    公开(公告)日：2007-01-23

    申请号：US10129627

    申请日：2000-11-09

    Applicant: Guillermina Hernandez ,  Alan Francois ,  Pascal Piveteau ,  Francoise Fayolle ,  Frederic Monot

    Inventor： Guillermina Hernandez ,  Alan Francois ,  Pascal Piveteau ,  Francoise Fayolle ,  Frederic Monot

    IPC: C12S13/00

    CPC classification number: C02F3/34 ,  Y10S435/821 ,  Y10S435/863

    Abstract: A process for the treatment of aqueous effluents containing at least one of the following ethers is described: ethyl tert-butyl ether (ETBE) and/or methyl tert-butyl ether (MTBE) and/or tert-amyl methyl ether (TAME) in order to reduce the concentration of these ethers. A bacterium Gordonia terrae CIP I-2194 is innoculated under aerobic conditions. In particular, a bacterium Burkholderia cepacia CIP I-2052 or a bacterium Alcaligenes sp. CIP I-2561 or a bacterium Mycobacterium sp. CIP I-2562 is added in the presence of a growth substrate and, optionally, of a cobalt salt, and the ether contained in the effluents is degraded by the bacteria thus innoculated until its mineralization. The process is useable in the ether-contaminated water treatment industry.

    Abstract translation: 描述了用于处理含有至少一种以下醚的含水废水的方法：乙基叔丁基醚（ETBE）和/或甲基叔丁基醚（MTBE）和/或叔戊基甲基醚（TAME）在 以降低这些醚的浓度。 细菌Gordonia terrae CIP I-2194在有氧条件下接种。 特别是洋葱伯克霍尔德菌CIP I-2052或细菌产碱杆菌 CIP I-2561或细菌分枝杆菌 在生长底物和任选的钴盐的存在下加入CIP I-2562，并且包含在流出物中的醚被细菌降解，直到其矿化为止。 该方法可用于醚类污水处理行业。

    METHOD OF PRODUCING ALCOHOL IN THE BIOREFINERY CONTEXT
    4.
    发明申请
    METHOD OF PRODUCING ALCOHOL IN THE BIOREFINERY CONTEXT 审中-公开
    在生物环境中生产酒精的方法

    公开(公告)号：US20100297717A1

    公开(公告)日：2010-11-25

    申请号：US12743410

    申请日：2008-11-06

    Applicant: Antoine Margeot ,  Frederic Monot

    Inventor： Antoine Margeot ,  Frederic Monot

    IPC: C12P7/16 ,  C12P7/02

    CPC classification number: C12P7/10 ,  C12F3/10 ,  C12P7/04 ,  C12P7/16 ,  Y02E50/10 ,  Y02E50/16

    Abstract: The present invention describes a method of producing alcohol from pretreated lignocellulosic biomass wherein the enzymatic hydrolysis stage is carried out with cellulolytic and/or hemicellulolytic enzymes produced using at least one effluent from another ethanol production process using a sugar plant as the feedstock. This method can be integrated in a method of producing alcohol from cellulosic or lignocellulosic materials, referred to as second-generation method, comprising the following stages: 1) chemical and/or physical pretreatment of a cellulosic or lignocellulosic substrate, 2) enzymatic hydrolysis of the pretreated substrate using cellulolytic and/or hemicellulolytic enzymes, 3) alcoholic fermentation by a suitable alcohol-producing microorganism of the hydrolysate from stage (2) until a fermentation must is obtained, and separation of the alcohol-producing microorganism used in stage (3), separation/purification of the alcohol.

    Abstract translation: 本发明描述了一种从预处理的木质纤维素生物质生产醇的方法，其中酶水解阶段是使用使用糖植物作为原料的来自另一种乙醇生产方法的至少一种流出物生产的纤维素分解和/或半纤维素分解酶进行的。 该方法可以整合在由纤维素或木质纤维素材料生产醇的方法中，称为第二代方法，包括以下阶段：1）纤维素或木质纤维素底物的化学和/或物理预处理，2）酶水解 使用纤维素分解和/或半纤维素分解酶的预处理底物，3）必须得到从阶段（2）到发酵的水解产物的合适的产生醇的微生物的酒精发酵，并且分离第（3） ），酒精的分离/纯化。

    Enzymatic process for treating xanthan gums in order to improve the filterability of their aqueous solutions
    5.
    发明授权
    Enzymatic process for treating xanthan gums in order to improve the filterability of their aqueous solutions 失效
    用于处理黄原胶的酶法，以改善其水溶液的过滤性

    公开(公告)号：US4904586A

    公开(公告)日：1990-02-27

    申请号：US46223

    申请日：1987-05-05

    Applicant: Daniel Ballerini ,  Yves Benoit ,  Frederic Monot

    Inventor： Daniel Ballerini ,  Yves Benoit ,  Frederic Monot

    IPC: C09K3/00 ,  C12P19/06 ,  C12P19/14 ,  E21B43/16

    CPC classification number: C12P19/06

    Abstract: Process for treating a xanthan gum in order to improve the filterability of its aqueous solutions, comprising an enzymatic treatment of an aqueous solution of xanthan gum containing, as dissolved salts, a proportion of alkali and/or alkaline-earth metals of at least 10.sup.-2 equivalent/liter, said treatment being performed by means of two enzyme extracts of different types, a so-called PG enzyme having as main activity a polygalacturonase activity and a so-called P enzyme extract whose main activity is a protease activity, in conditions compatible with the activity of said enzyme extracts. The obtained xanthan gum powder or solution can be used as enhanced oil recovery agent.

    Abstract translation: 用于处理黄原胶以提高其水溶液的过滤性的方法，包括将黄原胶水溶液的酶处理，所述黄原胶含有至少10重量份的碱金属和/或碱土金属的溶解盐， 2当量/升，所述处理通过两种不同类型的酶提取物进行，所谓的主要活性是多聚半乳糖醛酸酶活性的所谓的PG酶和主要活性是蛋白酶活性的所谓的P酶提取物，在条件 与所述酶提取物的活性相容。 获得的黄原胶粉末或溶液可用作增强的油回收剂。

    Process for the production of cellulolytic and/or hemicellulolytic enzymes
    6.
    发明授权
    Process for the production of cellulolytic and/or hemicellulolytic enzymes 有权

    公开(公告)号：US10457925B2

    公开(公告)日：2019-10-29

    申请号：US15684633

    申请日：2017-08-23

    Applicant: Fadhel Ben Chaabane ,  Frederic Monot

    Inventor： Fadhel Ben Chaabane ,  Frederic Monot

    IPC: C12N1/16 ,  C12N9/42 ,  C12N1/14 ,  C12P21/02 ,  C12N9/24

    Abstract: The process for the production of cellulolytic and/or hemicellulolytic enzymes by a cellulolytic and/or hemicellulolytic microorganism according to the present invention comprises at least one phase for growth in the presence of a source of carbon and at least one phase for production in the presence of an inducing substrate, in which said inducing substrate is a mixture comprising 40% to 65% by weight of glucose or cellulosic hydrolysates, 21% to 25% by weight of lactose and 10% to 39% by weight of xylose or a solution of a lignocellulosic hemicellulosic hydrolysate, the sum of these three constituents being equal to 100%.

    PROCESS FOR THE PRODUCTION OF CELLULOLYTIC AND/OR HEMICELLULOLYTIC ENZYMES
    7.
    发明申请
    PROCESS FOR THE PRODUCTION OF CELLULOLYTIC AND/OR HEMICELLULOLYTIC ENZYMES 审中-公开

    公开(公告)号：US20170349888A1

    公开(公告)日：2017-12-07

    申请号：US15684633

    申请日：2017-08-23

    Applicant: Fadhel BEN CHAABANE ,  Frederic MONOT

    Inventor： Fadhel BEN CHAABANE ,  Frederic MONOT

    IPC: C12N9/42 ,  C12P21/02 ,  C12N1/14 ,  C12N9/24

    Abstract: The process for the production of cellulolytic and/or hemicellulolytic enzymes by a cellulolytic and/or hemicellulolytic microorganism according to the present invention comprises at least one phase for growth in the presence of a source of carbon and at least one phase for production in the presence of an inducing substrate, in which said inducing substrate is a mixture comprising 40% to 65% by weight of glucose or cellulosic hydrolysates, 21% to 25% by weight of lactose and 10% to 39% by weight of xylose or a solution of a lignocellulosic hemicellulosic hydrolysate, the sum of these three constituents being equal to 100%.

    Polypeptides Having an Activity in the Mtbe Degradation Path and Uses Thereof
    9.
    发明申请
    Polypeptides Having an Activity in the Mtbe Degradation Path and Uses Thereof 有权
    在Mtbe降解途径中具有活性的多肽及其用途

    公开(公告)号：US20080210631A1

    公开(公告)日：2008-09-04

    申请号：US11996127

    申请日：2006-07-18

    Applicant: Nicolas Lopes Frreira ,  Diane Labbe ,  Helena Maciel ,  Francoise Fayolle-Guichard ,  Frederic Monot ,  Charles W. Greer

    Inventor： Nicolas Lopes Frreira ,  Diane Labbe ,  Helena Maciel ,  Francoise Fayolle-Guichard ,  Frederic Monot ,  Charles W. Greer

    IPC: C02F3/34 ,  C07K14/35 ,  C12N9/04 ,  C07H21/04 ,  C12N15/63 ,  C12N1/00 ,  C12N1/21 ,  C12Q1/68 ,  C12Q1/02 ,  C40B60/00

    CPC classification number: C02F3/34 ,  C12N9/0006 ,  C12N9/0008

    Abstract: The invention concerns an isolated or purified polypeptide having an activity in the MTBE degradation path, and/or at least one of the catabolic intermediates of MTBE, preferably selected from the group consisting of tert-butyl alcohol (TBA), 2-methyl 1,2-propanediol (2-M1, 2-PD), hydroxyisobutyraldehyde, hydroxyiso-butyric acid (HIBA), said polypeptide being selected from the group consisting of: a) a polypeptide comprising a sequence of amino acids selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 or SEQ ID NO:10; b) a polypeptide comprising a sequence of amino acids having at least 70% identity, preferably 75%, 80%, 90%, 95%, 98% or 99% with the sequence of amino acids of a polypeptide as defined in a); a polypeptide as defined in a) or b) whereof the sequence of amino acids comprises a substitution, deletion, insertion, addition or mutation of one or several amino acids over its entire length.

    Abstract translation: 本发明涉及在MTBE降解途径中具有活性的分离或纯化的多肽，和/或MTBE的分解代谢中间体中的至少一种，优选选自叔丁醇（TBA），2-甲基1， 2-丙二醇（2-M1，2-PD），羟基异丁醛，羟基异丁酸（HIBA），所述多肽选自：a）包含选自下组的氨基酸序列的多肽 SEQ ID NO：2，SEQ ID NO：4，SEQ ID NO：6，SEQ ID NO：8或SEQ ID NO：10; b）包含与a）中所定义的多肽的氨基酸序列具有至少70％同一性，优选75％，80％，90％，95％，98％或99％的氨基酸序列的多肽; a）或b）中定义的多肽，其中氨基酸序列包括其整个长度上一个或几个氨基酸的取代，缺失，插入，添加或突变。

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