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1.发明授权Insertion of the Bacillus thuringiensis crystal protein gene into plant colonizing microorganisms and their use 失效将苏云金芽孢杆菌晶体蛋白基因插入植物定植微生物及其应用公开(公告)号：US06197747B1
公开(公告)日：2001-03-06
申请号：US09343867
申请日：1999-06-30
Applicant: Lidia S. Watrud , Frederick J. Perlak
Inventor： Lidia S. Watrud , Frederick J. Perlak
IPC: A61K3800
CPC classification number: C07K14/325 , A01N63/00 , C12N15/78
Abstract: The invention relates to genetically engineered plant-colonizing microorganisms which proliferate in symbiotic or non-detrimental relationships with the plant in the plant environment. Such microorganisms contain DNA derived from Bacillus thuringiensis which codes for the insecticidal crystal protein toxin. The engineered plant-colonizing microorganisms of the invention and their progency are active against a variety of lepidopterous pests. The invention further relates to the use of such plant-colonizing microorganisms in a method of killing or inhibiting lepidopterous pests and to insecticidal compositions containing the plant-colonizing microorganism as the active insecticidal agent.
Abstract translation: 本发明涉及在植物环境中与植物共生或非有害关系的基因工程植物定植微生物。 这样的微生物含有源自苏云金芽孢杆菌的DNA，其编码杀虫晶体蛋白毒素。 本发明的工程植物定植微生物及其进展对各种鳞翅目害虫具有活性。 本发明还涉及这种植物定植微生物在杀死或抑制鳞翅目害虫的方法中的用途以及含有植物定植微生物作为活性杀虫剂的杀虫组合物。
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公开(公告)号：US20110307978A1
公开(公告)日：2011-12-15
申请号：US13114026
申请日：2011-05-23
Applicant: Natalia N. Bogdanova , David R. Corbin , Thomas M. Malvar , Frederick J. Perlak , James K. Roberts , Charles P. Romano
Inventor： Natalia N. Bogdanova , David R. Corbin , Thomas M. Malvar , Frederick J. Perlak , James K. Roberts , Charles P. Romano
CPC classification number: C07K14/325 , C12N15/8286 , Y02A40/162
Abstract: The present invention provides nucleotide sequences encoding an insecticidal protein exhibiting lepidopteran inhibitory activity, as well as a novel insecticidal protein referred to herein as a Cry1A.105 insecticide, transgenic plants expressing the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.
Abstract translation: 本发明提供编码表现鳞翅目昆虫抑制活性的杀虫蛋白的核苷酸序列，以及本文称为Cry1A.105杀虫剂的新型杀虫蛋白，表达杀虫剂的转基因植物，以及检测核苷酸序列或 生物样品中的杀虫剂。
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公开(公告)号：US06284949B1
公开(公告)日：2001-09-04
申请号：US09027998
申请日：1998-02-23
Applicant: David A. Fischhoff , Roy L. Fuchs , Paul B. Lavrik , Sylvia A. McPherson , Frederick J. Perlak
Inventor： David A. Fischhoff , Roy L. Fuchs , Paul B. Lavrik , Sylvia A. McPherson , Frederick J. Perlak
IPC: A01H500
CPC classification number: C12N15/78 , C07K14/325 , C12N15/8286 , Y02A40/162
Abstract: A method for producing genetically transformed plants exhibiting toxicity to Coleopteran insects is disclosed. In another aspect, the present invention embraces chimeric plant genes, genetically transformed cells and differentiated plants which exhibit toxicity to Coleopteran insects. In yet another aspect, the present invention embraces bacterial cells and plant transformation vectors comprising a chimeric plant gene encoding a Coleopteran toxin protein of Bacillus thuringiensis.
Abstract translation: 公开了一种生产对鞘翅目昆虫具有毒性的遗传转化植物的方法。 另一方面，本发明包括嵌合植物基因，遗传转化细胞和分化植物，其对鞘翅目昆虫具有毒性。 另一方面，本发明包括细菌细胞和植物转化载体，其包含编码苏云金芽孢杆菌的鞘翅目毒素蛋白质的嵌合植物基因。
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4.发明授权
公开(公告)号：US5959091A
公开(公告)日：1999-09-28
申请号：US917925
申请日：1986-10-10
Applicant: Lidia S. Watrud , Frederick J. Perlak
Inventor： Lidia S. Watrud , Frederick J. Perlak
IPC: A01G13/00 , A01C1/00 , A01N63/00 , C07K14/325 , C12N1/20 , C12N1/21 , C12N15/00 , C12N15/09 , C12N15/78 , C12P21/02 , C12R1/01 , C12R1/05 , C12R1/07 , C12R1/18 , C12R1/20 , C12R1/22 , C12R1/38 , C12R1/39 , C07H21/04
CPC classification number: C07K14/325 , A01N63/00 , C12N15/78
Abstract: The invention relates to genetically engineered plant-colonizing microorganisms which prolife-rate in symbiotic or non-detrimental relationships with the plant in the plant environment. Such microorganisms contain DNA derived from Bacillus thuringiensis which codes for the insecticidal crystal protein toxin. The engineered plant-colonizing microorganisms of the invention and their progeny are active against a variety of lepidopterous pests. The invention further relates to the use of such plant-colonizing microorganisms in a method of killing or inhibiting lepidopterous pests and to insecticidal compositions containing the plant-colonizing microorganism as the active insecticidal agent.
Abstract translation: 本发明涉及与植物环境中的植物共生或非有害关系的遗传工程植物定植微生物。 这样的微生物含有源自苏云金芽孢杆菌的DNA，其编码杀虫晶体蛋白毒素。 本发明的工程植物定植微生物及其后代对各种鳞翅目害虫具有活性。 本发明还涉及这种植物定植微生物在杀死或抑制鳞翅目害虫的方法中的用途以及含有植物定植微生物作为活性杀虫剂的杀虫组合物。
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公开(公告)号：US06953835B2
公开(公告)日：2005-10-11
申请号：US09943692
申请日：2001-08-31
Applicant: David A. Fischhoff , Roy L. Fuchs , Paul B. Lavrik , Sylvia A. McPherson , Frederick J. Perlak
Inventor： David A. Fischhoff , Roy L. Fuchs , Paul B. Lavrik , Sylvia A. McPherson , Frederick J. Perlak
IPC: A01H1/00 , A01H5/00 , C07K14/325 , C07K14/37 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/32 , C12N15/35 , C12N15/78 , C12N15/82 , C12P21/02 , C12R1/07 , C07N14/325 , A01N37/52
CPC classification number: C12N15/78 , C07K14/325 , C12N15/8286 , Y02A40/162
Abstract: A method for producing genetically transformed plants exhibiting toxicity to Coleopteran insects is disclosed. In another aspect, the present invention embraces chimeric plant genes, genetically transformed cells and differentiated plants which exhibit toxicity to Coleopteran insects. In yet another aspect, the present invention embraces bacterial cells and plant transformation vectors comprising a chimeric plant gene encoding a Coleopteran toxin protein of Bacillus thuringiensis.
Abstract translation: 公开了一种生产对鞘翅目昆虫具有毒性的遗传转化植物的方法。 另一方面，本发明包括嵌合植物基因，遗传转化细胞和分化植物，其对鞘翅目昆虫具有毒性。 另一方面，本发明包括细菌细胞和植物转化载体，其包含编码苏云金芽孢杆菌的鞘翅目毒素蛋白质的嵌合植物基因。
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Patent Agency Ranking6.发明授权
公开(公告)号：US5880275A
公开(公告)日：1999-03-09
申请号：US841178
申请日：1997-04-29
Applicant: David A. Fischhoff , Frederick J. Perlak
Inventor： David A. Fischhoff , Frederick J. Perlak
IPC: A01H5/00 , A01H5/10 , C07K14/08 , C07K14/325 , C07K14/415 , C12N5/02 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/32 , C12N15/40 , C12N15/67 , C12N15/82 , C12R1/07
CPC classification number: C07K14/005 , C07K14/325 , C07K14/415 , C12N15/67 , C12N15/8216 , C12N15/8283 , C12N15/8286 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C07K2319/06 , C07K2319/08 , C12N2770/00022
Abstract: A method for modifying structural gene sequences to enhance the expression of the protein product is disclosed. Also disclosed are novel structural genes which encode insecticidal proteins of B.t.k. HD-1, and B.t.k. HD-73.
Abstract translation: 公开了一种修饰结构基因序列以增强蛋白质产物表达的方法。 还公开了编码B.t.k.的杀虫蛋白的新型结构基因。 HD-1和B.t.k. HD-73。
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7.发明授权Combatting plant insect pests with plant-colonizing microorganisms containing the toxin gene B. thuringiensis as a chromosomal insertion 失效将植物害虫与含有毒素基因苏云金芽孢杆菌作为染色体插入物的植物定植微生物相抗
公开(公告)号：US5229112A
公开(公告)日：1993-07-20
申请号：US363318
申请日：1989-06-07
Applicant: Mark G. Obukowicz , Frederick J. Perlak , Lidia S. Watrud
Inventor： Mark G. Obukowicz , Frederick J. Perlak , Lidia S. Watrud
CPC classification number: C12N15/90 , A01N63/00 , C07K14/325 , C12P1/04
Abstract: The invention relates to a plant-colonizing microorganism which has been genetically engineered to integrate into the chromosome of such microorganism, DNA derived from B. thuringiensis coding for protein toxin. The genetically engineered plant-colonizing microorganisms of the invention, and their progeny, proliferate in commensal or non-detrimental relationship with the plant in the plant environment and are insecticidally active against a subspecies of insect pest which are harmful to the plant. The invention further relates to insecticidal compositions containing such plant-colonizing microorganisms as the active insecticidal agent and to a method of using such genetically engineered plant-colonizing microorganisms in a method of killing or inhibiting insect pests.
Abstract translation: 本发明涉及一种植物定植微生物，其已被遗传工程化以整合到这种微生物的染色体中，来自编码蛋白质毒素的苏云金芽孢杆菌的DNA。 本发明的遗传工程植物定植微生物及其后代与植物环境中植物的共生或非有害关系增殖，并且对于对植物有害的害虫亚种具有杀虫活性。 本发明还涉及含有作为活性杀虫剂的植物定植微生物的杀虫组合物，以及在杀死或抑制昆虫害虫的方法中使用这种基因工程植物定植微生物的方法。
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公开(公告)号：US08034997B2
公开(公告)日：2011-10-11
申请号：US12064875
申请日：2006-08-30
Applicant: Natalia N. Bogdanova , David R. Corbin , Thomas M. Malvar , Frederick J. Perlak , James K. Roberts , Charles P. Romano
Inventor： Natalia N. Bogdanova , David R. Corbin , Thomas M. Malvar , Frederick J. Perlak , James K. Roberts , Charles P. Romano
CPC classification number: C07K14/325 , C12N15/8286 , Y02A40/162
Abstract: The present invention provides nucleotide sequences encoding an insecticidal protein exhibiting lepidopteran inhibitory activity, as well as a novel insecticidal protein referred to herein as a Cry1A.105 insecticide, transgenic plants expressing the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.
Abstract translation: 本发明提供编码表现鳞翅目昆虫抑制活性的杀虫蛋白的核苷酸序列，以及本文称为Cry1A.105杀虫剂的新型杀虫蛋白，表达杀虫剂的转基因植物，以及检测核苷酸序列或 生物样品中的杀虫剂。
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公开(公告)号：US20090238798A1
公开(公告)日：2009-09-24
申请号：US12064875
申请日：2006-08-30
Applicant: Natalia N. Bogdanova , David R. Corbin , Thomas M. Malvar , Frederick J. Perlak , James K. Roberts , Charles P. Romano
Inventor： Natalia N. Bogdanova , David R. Corbin , Thomas M. Malvar , Frederick J. Perlak , James K. Roberts , Charles P. Romano
CPC classification number: C07K14/325 , C12N15/8286 , Y02A40/162
Abstract: The present invention provides nucleotide sequences encoding an insecticidal protein exhibiting lepidopteran inhibitory activity, as well as a novel insecticidal protein referred to herein as a Cry1A.105 insecticide, transgenic plants expressing the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.
Abstract translation: 本发明提供编码表现鳞翅目昆虫抑制活性的杀虫蛋白的核苷酸序列，以及本文称为Cry1A.105杀虫剂的新型杀虫蛋白，表达杀虫剂的转基因植物，以及检测核苷酸序列或 生物样品中的杀虫剂。
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公开(公告)号：US5500365A
公开(公告)日：1996-03-19
申请号：US959506
申请日：1992-10-09
Applicant: David A. Fischhoff , Frederick J. Perlak
Inventor： David A. Fischhoff , Frederick J. Perlak
IPC: A01H5/00 , A01H5/10 , C07K14/08 , C07K14/325 , C07K14/415 , C12N5/02 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/32 , C12N15/40 , C12N15/67 , C12N15/82 , C12R1/07
CPC classification number: C07K14/005 , C07K14/325 , C07K14/415 , C12N15/67 , C12N15/8216 , C12N15/8283 , C12N15/8286 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C07K2319/06 , C07K2319/08 , C12N2770/00022
Abstract: A method for modifying structural gene sequences to enhance the expression of the protein product is disclosed. Also disclosed are novel structural genes which encode insecticidal proteins of B.t.k. HD-1, B.t.k. HD-73, B.t. tenebrionis, B.t. entomocidus, 2 protein of B.t.k. HD-1, and the coat protein of potato leaf roll virus.
Abstract translation: 公开了一种修饰结构基因序列以增强蛋白质产物表达的方法。 还公开了编码B.t.k.的杀虫蛋白的新型结构基因。 HD-1，B.t.k. HD-73，B.t。 苔藓属 昆虫杆菌，B.t.k的2蛋白。 HD-1和马铃薯卷叶病毒的外壳蛋白。
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