公开(公告)号：US20110045492A1

公开(公告)日：2011-02-24

申请号：US12922573

申请日：2009-03-13

Applicant: Niels Kristian Bau-Madsen ,  Jacques Jonsmann ,  Bent Overby ,  Christianus Berendsen

Inventor： Niels Kristian Bau-Madsen ,  Jacques Jonsmann ,  Bent Overby ,  Christianus Berendsen

IPC: G01N33/566 ,  C12M1/34 ,  C12Q1/02 ,  C12Q1/56 ,  G01N33/86 ,  G01N21/00 ,  G01N21/59

CPC classification number: G01N33/4905 ,  B01L3/5027 ,  B01L2200/0684 ,  B01L2200/148 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/18 ,  B01L2400/0406 ,  B01L2400/0487 ,  G01N33/5304 ,  G01N33/86

Abstract: The invention relates to a microfluidic system comprising a microfluidic device having a first and a second opposite surfaces and an optical detector, the microfluidic device comprises a flow channel with a detection channel section having a length of at least about 1 mm, the microfluidic device comprises at least one aperture section comprising at least a part of said detection channel section and a transparent window into said detection channel section, the optical detector is arranged to be in optical communication with said aperture section to determining at least one optical property of said aperture section as a function of time. The flow channel may have capillary dimensions and/or it may wholly or fully be arranged to drive a fluid flow by applying external forces.The optical detector may preferably be arranged to transmit light onto the aperture section and to detect a light transmission from the aperture section, wherein the light transmission from the aperture section being selected from light transmitted through the sample, reflectance scattering of light, transmittance scattering of light and any combinations thereof.The microfluid device may be used to determine various properties of a sample fluid, for example it may be used to determine a samples coagulation properties and/or reactions of one or more components in a fluid sample as a function of time.

Abstract translation: 本发明涉及一种包括具有第一和第二相对表面的微流体装置和光学检测器的微流体系统，所述微流体装置包括具有至少约1mm长度的检测通道部分的流动通道，所述微流体装置包括 至少一个孔径部分，其包括所述检测通道部分的至少一部分和在所述检测通道部分中的透明窗口，所述光学检测器布置成与所述孔部分光学连通，以确定所述孔部分的至少一个光学特性 作为时间的函数。 流动通道可以具有毛细管尺寸和/或其可以完全或完全地布置成通过施加外力来驱动流体流动。 光检测器可以优选地布置成将光透射到孔部分上并且检测从孔部分的光透射，其中来自孔部分的光透射选自透过样品的光，光的反射散射，透射散射 光及其任何组合。 微流体装置可用于确定样品流体的各种性质，例如其可用于确定样品凝固性质和/或流体样品中一种或多种组分与时间的关系的反应。

公开(公告)号：US20100099097A1

公开(公告)日：2010-04-22

申请号：US12532282

申请日：2008-03-17

Applicant: Jacques JONSMANN

Inventor： Jacques JONSMANN

IPC: C12Q1/68 ,  G01N33/53 ,  C12M1/34

CPC classification number: G01N33/558 ,  B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2400/0406 ,  G01N33/54366

Abstract: The invention relates to a flow through system for quantifying a target component in a liquid. The flow through system comprises a flow-through device comprising a flow path comprising a marker section, a capture section downstream to said marker section, and at least two quantification sections. The marker section comprises a non-immobilized marker. The capture section comprises a capture zone with an immobilized capture agent, and the at least two quantification sections comprise a pre-capture quantification section placed downstream to the marker section and up stream to the capture section, and a post-capture quantification section placed downstream to the capture section. The system further comprises a quantification unit for each of said quantification sections. The quantification unit(s) being arranged to quantify marker containing components and/or particles passing through said respective quantification sections. The invention also relates to a flow through device for such flow through system and a method of determining the amount of target component in a liquid using a flow through system.

Abstract translation: 本发明涉及一种用于量化液体中的目标成分的流通系统。 流过系统包括流通装置，其包括流路，该流路包括标记部分，所述标记部分下游的捕获部分和至少两个定量部分。 标记部分包括非固定化标记。 捕获部分包括具有固定的捕获剂的捕获区，并且所述至少两个定量部分包含放置在标记部分下游的捕获前定量部分和向捕获部分的上游，以及放置在下游的捕获后定量部分 到捕获部分。 该系统还包括用于每个所述量化部分的量化单元。 定量单元被布置成量化通过所述各个量化部分的含有标记物的组分和/或颗粒。 本发明还涉及用于这种流通系统的流通装置和使用流通系统确定液体中的目标成分的量的方法。

公开(公告)号：US09201059B2

公开(公告)日：2015-12-01

申请号：US12922573

申请日：2009-03-13

Applicant: Niels Kristian Bau-Madsen ,  Jacques Jonsmann ,  Bent Overby ,  Christianus Berendsen

Inventor： Niels Kristian Bau-Madsen ,  Jacques Jonsmann ,  Bent Overby ,  Christianus Berendsen

IPC: B01L3/00 ,  G01N33/49 ,  G01N33/53 ,  G01N33/86

CPC classification number: G01N33/4905 ,  B01L3/5027 ,  B01L2200/0684 ,  B01L2200/148 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/18 ,  B01L2400/0406 ,  B01L2400/0487 ,  G01N33/5304 ,  G01N33/86

Abstract: The invention relates to a microfluidic system comprising a microfluidic device having a first and a second opposite surfaces and an optical detector, the microfluidic device comprises a flow channel with a detection channel section having a length of at least about 1 mm, the microfluidic device comprises at least one aperture section comprising at least a part of said detection channel section and a transparent window into said detection channel section, the optical detector is arranged to determine at least one optical property of said aperture section as a function of time. The flow channel may have capillary dimensions and/or it may wholly or fully be arranged to drive a fluid flow by applying external forces. The microfluid device may be used to determine various properties of a sample fluid, for example it may be used to determine a samples coagulation properties and/or reactions of one or more components in a fluid sample as a function of time.

Abstract translation: 本发明涉及一种包括具有第一和第二相对表面的微流体装置和光学检测器的微流体系统，所述微流体装置包括具有至少约1mm长度的检测通道部分的流动通道，所述微流体装置包括 至少一个孔径部分，其包括所述检测通道部分的至少一部分和在所述检测通道部分中的透明窗口，所述光学检测器被布置成根据时间确定所述孔径部分的至少一个光学特性。 流动通道可以具有毛细管尺寸和/或其可以完全或完全地布置成通过施加外力来驱动流体流动。 微流体装置可用于确定样品流体的各种性质，例如其可用于确定样品凝固性质和/或流体样品中一种或多种组分与时间的关系的反应。

公开(公告)号：US20050089803A1

公开(公告)日：2005-04-28

申请号：US10478947

申请日：2002-04-25

Applicant: Salim Bouaidat ,  Jacques Jonsmann ,  Bjorn Winther-Jensen ,  Soron Christensen

Inventor： Salim Bouaidat ,  Jacques Jonsmann ,  Bjorn Winther-Jensen ,  Soron Christensen

IPC: G03F7/26 ,  B05D7/24 ,  C08G83/00 ,  C12M1/40 ,  C12N5/00 ,  G03F7/00 ,  G03F7/40 ,  G03C5/00 ,  H01L21/302

CPC classification number: C12N5/0068 ,  C12N2533/30 ,  C12N2535/10 ,  G03F7/00

Abstract: Methods and apparatus for lift-off microstructuring deposition material, here alternate hydrophilic (601) and hydrophobic (602) letters, on a substrate; a method comprising deposition of polymeric material by plasma polymerisation deposition of monomers of substituted benzenes, (halo)aliphatic compounds, or a combination thereof; another method comprising deposition of polymeric material by plasma polymerisation deposition of monomers of vinyls, substituted vinyls, acrylics, silanes, and phosphites, or a combination thereof; still another method comprising deposition of polymeric material by plasma polymerisation deposition of monomers wherein said plasma is generated by a multiple phase AC supply, or DC supply; and substrates and devices prepared by lift-off microstructuring using plasma polymerisation deposition of monomers according to such methods. Scale A indicates about 100μ.

Abstract translation: 用于剥离微结构沉积材料的方法和装置，这里是在基底上的交替的亲水（601）和疏水（602）字母; 一种方法，包括通过等离子体聚合沉积聚合物材料沉积取代的苯，（卤代）脂族化合物或其组合的单体; 另一种方法包括通过乙烯基，取代的乙烯基，丙烯酸，硅烷和亚磷酸酯的单体的等离子体聚合沉积或其组合沉积聚合物材料; 另一种方法包括通过等离子体聚合沉积单体沉积聚合物材料，其中所述等离子体由多相AC电源或DC电源产生; 以及通过使用根据这些方法的等离子体聚合沉积单体的剥离显微结构制备的底物和装置。 比例A表示约100mu。

公开(公告)号：US08865454B2

公开(公告)日：2014-10-21

申请号：US12532282

申请日：2008-03-17

Applicant: Jacques Jonsmann

Inventor： Jacques Jonsmann

IPC: C12M1/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N21/75 ,  G01N21/77 ,  G01N33/543 ,  G01N33/558 ,  B01L3/00

CPC classification number: G01N33/558 ,  B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2400/0406 ,  G01N33/54366

Abstract: The invention relates to a flow through system for quantifying a target component in a liquid. The flow through system comprises a flow-through device comprising a flow path comprising a marker section, a capture section downstream to said marker section, and at least two quantification sections. The marker section comprises a non-immobilized marker. The capture section comprises a capture zone with an immobilized capture agent, and the at least two quantification sections comprise a pre-capture quantification section placed downstream to the marker section and up stream to the capture section, and a post-capture quantification section placed downstream to the capture section. The system further comprises a quantification unit for each of said quantification sections. The quantification unit(s) being arranged to quantify marker containing components and/or particles passing through said respective quantification sections. The invention also relates to a flow through device for such flow through system and a method of determining the amount of target component in a liquid using a flow through system.

Abstract translation: 本发明涉及一种用于量化液体中的目标成分的流通系统。 流过系统包括流通装置，其包括流路，该流路包括标记部分，所述标记部分下游的捕获部分和至少两个定量部分。 标记部分包括非固定化标记。 捕获部分包括具有固定的捕获剂的捕获区，并且所述至少两个定量部分包含放置在标记部分下游的捕获前定量部分和向捕获部分的上游，以及放置在下游的捕获后定量部分 到捕获部分。 该系统还包括用于每个所述定量部分的量化单元。 定量单元被布置成量化通过所述各个量化部分的含有标记物的组分和/或颗粒。 本发明还涉及用于这种流通系统的流通装置和使用流通系统确定液体中的目标成分的量的方法。

公开(公告)号：US20110143383A1

公开(公告)日：2011-06-16

申请号：US13056420

申请日：2009-07-29

Applicant: Jacques Jonsmann ,  Niels Kristian Bau-Madsen

Inventor： Jacques Jonsmann ,  Niels Kristian Bau-Madsen

IPC: C12Q1/56 ,  B01L3/00 ,  C12Q1/00 ,  G01N1/10

CPC classification number: B01L3/502746 ,  B01L3/502723 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2400/0406 ,  B01L2400/084 ,  Y10T436/2575

Abstract: A microfluidic device comprising a flow channel with an inlet and a gas escape opening is described. The flow channel comprises a liquid flow channel section and a flow controlling section downstream to the liquid flow channel section and upstream to or coinciding with the gas escape opening. The flow controlling section provides a flow resistance to gas, which is sufficiently high to reduce velocity of a capillary flow of a liquid in the liquid flow channel section.The microfluidic device with the flow controlling section provides a device in which the velocity of the flow can be reduced to a desired level.Also is described a method of performing a test using the microfluidic device.

Abstract translation: 描述了包括具有入口和气体逸出开口的流动通道的微流体装置。 流动通道包括液体流动通道部分和流体控制部分，其位于液体流动通道部分的下游，并且与气体逸出口上游或与气体逸出口重合。 流量控制部分提供对气体的流动阻力，其足够高以降低液体流动通道部分中液体的毛细管流速。 具有流量控制部分的微流体装置提供了一种可以将流速降低到期望水平的装置。 还描述了使用微流体装置进行测试的方法。

公开(公告)号：US20100089529A1

公开(公告)日：2010-04-15

申请号：US11813835

申请日：2006-01-11

Applicant: Claus Barholm-Hansen ,  Jacques Jonsmann ,  Thomas Rosleff Baekmark ,  Bent Overby ,  Christian Berendsen ,  Salim Bouaidat ,  Niels Kristian Bau-Madsen

Inventor： Claus Barholm-Hansen ,  Jacques Jonsmann ,  Thomas Rosleff Baekmark ,  Bent Overby ,  Christian Berendsen ,  Salim Bouaidat ,  Niels Kristian Bau-Madsen

IPC: B32B38/10

CPC classification number: F16K99/0017 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/165 ,  B01L2400/0406 ,  B01L2400/0688 ,  F16K99/0001 ,  F16K2099/0078

Abstract: A method of producing a microfluidic device having at least one flow path may include providing a base substrate with a first surface and a top substrate with a second surface, hydrophilically treating at least one of the first and the second surfaces to provide a surface layer with a higher surface tension than the surface tension prior to the hydrophilic treatment, partly or totally removing the surface layer with a higher surface tension in a selected pattern of the hydrophilically treated first and/or second surfaces, to thereby provide the selected pattern with a lower surface tension than prior to the partly or totally removal of the surface layer with a higher surface tension in said selected pattern of the hydrophilic treated first and/or second surfaces, and joining said base substrate and top substrate to each other to provide a flow path between said first and second surfaces.

Abstract translation: 一种制造具有至少一个流路的微流体装置的方法可包括提供具有第一表面的基底基底和具有第二表面的顶部基底，亲水处理第一和第二表面中的至少一个以提供表面层 在亲水处理之前具有比表面张力更高的表面张力，部分地或全部地在亲水处理的第一和/或第二表面的选定图案中以更高的表面张力去除表面层，从而为所选择的图案提供较低的表面张力 表面张力比在亲水处理的第一和/或第二表面的所述选定图案中部分地或完全去除表面层之前具有更高表面张力的表面张力，以及将所述基底基板和顶部基板彼此接合以提供流动路径 在所述第一和第二表面之间。

公开(公告)号：US20050164118A1

公开(公告)日：2005-07-28

申请号：US10503161

申请日：2003-01-31

Applicant: Claus Barholm -Hansen ,  Jacques Jonsmann

Inventor： Claus Barholm -Hansen ,  Jacques Jonsmann

IPC: B01J19/00 ,  B01L3/00 ,  B81C1/00 ,  G03F7/00 ,  G03F7/038 ,  G03F7/40 ,  G03C1/76

CPC classification number: B81C3/001 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00833 ,  B01L3/5027 ,  B81B2201/058 ,  B81B2203/0338 ,  B81C1/00357 ,  B81C2201/019 ,  G03F7/0012 ,  G03F7/0385 ,  G03F7/40

Abstract: A method of joining a workpiece and a microstructure by light exposure, a microstructure obtainable by the method comprising a workpiece joined thereto, means thereto and use thereof; in particular a microstructure-forming composition comprising a light-sensitive, structure-forming material comprising one or more photo resist materials which are sensitive to preferably UV-light, and a light-absorbing material comprising one or more light-absorbing substances absorbing preferably IR light and being in an amount sufficient to produce heat upon exposure to said absorbed light; a microstructure-forming preparation comprising such composition; a method of producing a microstructure on a substrate; and a microstructure obtainable by the method; a method of joining a workpiece and a microstructure, a microstructure obtainable by the method comprising a workpiece joined thereto, e.g. for producing closed micro flow channels in a micro flow system; and use of such a microstructure, e.g. in lab-on-chip applications, in point-of-care systems, in high-through-put screening systems, preferably in systems for screening active compounds in fluids, in particular biological fluids.

Abstract translation: 通过曝光接合工件和微结构的方法，通过包括与其接合的工件的方法可获得的微观结构及其用途; 特别是一种微结构形成组合物，其包含光敏的结构形成材料，其包含对优选紫外光敏感的一种或多种光致抗蚀剂材料，以及光吸收材料，其包含一种或多种吸收物质优选吸收IR 并且其量足以在暴露于所述吸收的光时产生热量; 包含这种组合物的微结构形成制剂; 在基板上制造微观结构的方法; 和通过该方法获得的微结构; 接合工件和微结构的方法，通过包括与其连接的工件的方法获得的微结构，例如。 用于在微流量系统中产生闭合的微流通道; 并使用这种微结构，例如。 在片上应用中，在点到点系统中，在高通量筛选系统中，优选用于筛选流体中特别是生物流体中的活性化合物的系统。

公开(公告)号：US08877484B2

公开(公告)日：2014-11-04

申请号：US12522756

申请日：2008-01-04

Applicant: Niels Kristian Bau-Madsen ,  Jacques Jonsmann ,  Søren Larsen

Inventor： Niels Kristian Bau-Madsen ,  Jacques Jonsmann ,  Søren Larsen

IPC: C12M1/00 ,  B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/502738 ,  B01L3/502769 ,  B01L2200/0621 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0688 ,  Y10T436/143333

Abstract: A microfluidic device comprising at least one test channel, which test channel comprises an upper test channel section with an upstream end and a sampling region at its upstream end and at least one reference channel, which reference channel comprises an upper reference channel section with an upstream end and a sampling region at its upstream end. The test channel and the reference channel comprise a merging region downstream to the upper test channel section and a common downstream channel section. The merging region and the common downstream channel section are arranged such that a reference liquid flowing from the upper reference channel section into the merging region will block a test liquid flow in the upper test channel section when the test liquid flow has not yet reached the merging section. The microfluid device may be used for detecting change of flow properties e.g. due to agglomeration, agglutination or viscosity change in a liquid preferably selected from water, urine, blood, or blood plasma.

Abstract translation: 一种微流体装置，包括至少一个测试通道，所述测试通道包括具有上游端的上测试通道部分和其上游端的采样区域以及至少一个参考通道，所述参考通道包括具有上游的上参考通道部分 端和在其上游端的采样区。 测试信道和参考信道包括上测试信道部分和公共下行信道部分下游的合并区域。 合流区域和公共下游槽部分被布置成使得当测试液体流尚未达到合并时，从上部参考通道部分流入合并区域的参考液体将阻挡上部测试通道部分中的测试液体流动 部分。 微流体装置可以用于检测流动特性的变化，例如 由于优选选自水，尿，血液或血浆的液体中的聚集，凝集或粘度变化。

公开(公告)号：US20090317793A1

公开(公告)日：2009-12-24

申请号：US12522756

申请日：2008-01-04

Applicant: Jacques Jonsmann ,  Niels Kristian Bau-Madsen ,  Søren Larsen

Inventor： Jacques Jonsmann ,  Niels Kristian Bau-Madsen ,  Søren Larsen

IPC: C12Q1/00 ,  G01N33/49 ,  G01N33/00

CPC classification number: B01L3/50273 ,  B01L3/502738 ,  B01L3/502769 ,  B01L2200/0621 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0688 ,  Y10T436/143333

Abstract: A microfluidic device comprising at least one test channel, which test channel comprises an upper test channel section with an upstream end and a sampling region at its upstream end and at least one reference channel, which reference channel comprises an upper reference channel section with an upstream end and a sampling region at its upstream end. The test channel and the reference channel comprise a merging region downstream to the upper test channel section and a common downstream channel section. The merging region and the common downstream channel section are arranged such that a reference liquid flowing from the upper reference channel section into the merging region will block a test liquid flow in the upper test channel section when the test liquid flow has not yet reached the merging section. The microfluid device may be used for detecting change of flow properties e.g. due to agglomeration, agglutination or viscosity change in a liquid preferably selected from water, urine, blood, or blood plasma.

Abstract translation: 一种微流体装置，包括至少一个测试通道，所述测试通道包括具有上游端的上测试通道部分和其上游端的采样区域以及至少一个参考通道，所述参考通道包括具有上游的上参考通道部分 端和在其上游端的采样区。 测试信道和参考信道包括上测试信道部分和公共下行信道部分下游的合并区域。 合流区域和公共下游槽部分被布置成使得当测试液体流尚未达到合并时，从上部参考通道部分流入合并区域的参考液体将阻挡上部测试通道部分中的测试液体流动 部分。 微流体装置可以用于检测流动特性的变化，例如 由于优选选自水，尿，血液或血浆的液体中的聚集，凝集或粘度变化。