公开(公告)号：US4737251A

公开(公告)日：1988-04-12

申请号：US781283

申请日：1985-09-27

Applicant: Georges F. Carle ,  Maynard V. Olson

Inventor： Georges F. Carle ,  Maynard V. Olson

IPC: B01D57/02 ,  B01D61/00 ,  G01N27/26 ,  G01N27/327 ,  G01N27/447

CPC classification number: B01D57/02 ,  G01N27/44773

Abstract: Disclosed herein is a method and apparatus for gel electrophoresis which employs periodic inversion of the electric field essentially in one dimension. According to this system, which is defined herein as field-inversion gel electrophoresis, net migration is achieved by using a longer time or higher voltage in one direction than in the other direction.

Abstract translation: 本文公开了一种凝胶电泳的方法和装置，其基本上在一个维度上采用电场的周期性反转。 根据本文中定义为场反转凝胶电泳的该系统，通过在一个方向上比在另一个方向上使用更长的时间或更高的电压来实现净迁移。

公开(公告)号：US5167790A

公开(公告)日：1992-12-01

申请号：US526772

申请日：1990-05-18

Applicant: Georges F. Carle ,  Maynard V. Olson

Inventor： Georges F. Carle ,  Maynard V. Olson

IPC: B01D57/02 ,  G01N27/447

CPC classification number: B01D57/02 ,  G01N27/44773

Abstract: A method and apparatus for gel electrophoresis which employs periodic inversion of the electric field essentially in one dimension, denoted as field-inversion gel electrophoresis (FIGE), results in net migration by using a longer time or higher voltage in one direction than in the opposite direction. FIGE permits separation of DNA or protein mixtures in size ranges not accessible to ordinary electrophoresis.

Abstract translation: 凝胶电泳的方法和装置，其基本上在一维上采用电场周期反演，表示为场反转凝胶电泳（FIGE），通过在一个方向上使用比在相反方向上更长的时间或更高的电压来实现净迁移 方向。 FIGE允许在普通电泳不可接近的尺寸范围内分离DNA或蛋白质混合物。

公开(公告)号：US5785926A

公开(公告)日：1998-07-28

申请号：US531215

申请日：1995-09-19

Applicant: Ronald Seubert ,  Maynard V. Olson ,  Deirdre Meldrum ,  Blake Hannaford ,  Peter Wiktor ,  Neal A. Friedman ,  Donald B. Snow ,  Ray Kraft

Inventor： Ronald Seubert ,  Maynard V. Olson ,  Deirdre Meldrum ,  Blake Hannaford ,  Peter Wiktor ,  Neal A. Friedman ,  Donald B. Snow ,  Ray Kraft

IPC: B01F11/00 ,  B01F13/00 ,  B01F15/02 ,  B01L3/02 ,  B01L3/06 ,  G01N35/00

CPC classification number: B01F13/0071 ,  B01F11/0071 ,  B01F15/0232 ,  B01L3/02 ,  B01L3/022 ,  B01L3/0262 ,  B01L3/0268 ,  B01L3/06 ,  B01L9/065 ,  B01F13/0059 ,  B01F13/0084 ,  B01F15/0201 ,  B01F2215/0409 ,  B01L2200/0605 ,  B01L2200/143 ,  B01L2300/0838 ,  B01L2300/18 ,  B01L2400/0478 ,  G01N2035/00237 ,  Y10T436/118339 ,  Y10T436/119163 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: A high precision, small volume fluid processing system employs open ended capillary tubes to meter, aliquot and mix small volumes of sample fluid and reagents. The system has an automatic mechanism for moving the capillary tubes as well as automated sub-systems for incubating and mixing fluids within the capillary tubes.

Abstract translation: 高精度，小体积的流体处理系统采用开放式毛细管来计量，等分和混合少量的样品液和试剂。 该系统具有用于移动毛细管的自动机构以及用于在毛细管内孵育和混合流体的自动子系统。

公开(公告)号：US4889806A

公开(公告)日：1989-12-26

申请号：US38280

申请日：1987-04-15

Applicant: Maynard V. Olson ,  David T. Burke

Inventor： Maynard V. Olson ,  David T. Burke

IPC: C12N15/81

CPC classification number: C12N15/81 ,  Y10S435/942

Abstract: A large DNA cloning system is disclosed which is based on yeast artificial chromosomes. Cloning vectors are disclosed which allow the cloning of large segments of greater than 50 kb of exogenous DNA. The cloning vector comprises DNA sequences of an autonomous replication sequence, a centromere, a selectable yeast marker, two sequences that seed telomere function in vivo, and a cloning site within an interruptible yeast gene for insertion of the exogenous DNA segments.

Abstract translation: 公开了一种基于酵母人造染色体的大型DNA克隆系统。 公开了克隆载体，其允许克隆大于50kb的外源DNA的大片段。 克隆载体包括自主复制序列，着丝粒，可选择的酵母标记，种子端粒在体内功能的两个序列的DNA序列，以及用于插入外源DNA片段的可中断酵母基因内的克隆位点。