公开(公告)号：US07801766B2

公开(公告)日：2010-09-21

申请号：US09822961

申请日：2001-03-30

Applicant: Mark Bunger ,  Michael Reiling ,  Michael Comb ,  Edward Bank ,  Michael McCaffery ,  Carolee A. Reiling ,  Juan Carlos Velten

Inventor： Mark Bunger ,  Michael Reiling ,  Michael Comb ,  Edward Bank ,  Michael McCaffery ,  Carolee A. Reiling ,  Juan Carlos Velten

IPC: G06Q30/00

CPC classification number: G06Q30/02 ,  G06Q20/20 ,  G06Q20/201 ,  G06Q20/202 ,  G06Q30/0207 ,  G06Q30/0224 ,  G06Q30/0234 ,  G06Q30/0236 ,  G06Q30/0633 ,  G06Q30/0641 ,  G06Q40/12

Abstract: A method and system for facilitating a transaction between at least one user, at least one merchant, at least one associate is provided. The method includes the steps of the associate processing device transferring into the user processing device a first merchant link for a first merchant Web site on a first merchant processing device. The first merchant link is framed with associate information. The user selects the first merchant link. The associate processing device transfers into the user processing device information regarding a first product from the first merchant Web site. The user selects the first product to purchase. The user provides purchase information for purchasing the first product. The user processing device transfers the purchase information to the associate processing device. The associate processing device adds promotional information to the purchase information responsive to the user identification value. The associate processing device transfers the promotional information and the purchase information to the merchant processing device.

Abstract translation: 提供了一种用于促进至少一个用户，至少一个商家，至少一个联系人之间的交易的方法和系统。 所述方法包括以下步骤：所述关联处理装置向所述用户处理装置传送第一商户处理装置上的第一商户网站的第一商家链接。 第一个商家链接框架有关联信息。 用户选择第一个商家链接。 关联处理装置从第一商户网站向用户处理装置传送关于第一商品的信息。 用户选择要购买的第一个产品。 用户提供购买第一个产品的购买信息。 用户处理装置将购买信息传送到关联处理装置。 辅助处理设备响应于用户识别值将促销信息添加到购买信息。 关联处理装置将促销信息和购买信息传送到商家处理装置。

公开(公告)号：US20090254432A1

公开(公告)日：2009-10-08

申请号：US12485634

申请日：2009-06-16

Applicant: Michael McCaffery ,  Mark Bunger ,  Michael Reiling ,  Michael Comb ,  Edward Bank ,  Carolee A. Reiling ,  Juan Carlos Velten

Inventor： Michael McCaffery ,  Mark Bunger ,  Michael Reiling ,  Michael Comb ,  Edward Bank ,  Carolee A. Reiling ,  Juan Carlos Velten

IPC: G06Q30/00

CPC classification number: G06Q30/02 ,  G06Q20/20 ,  G06Q20/201 ,  G06Q20/202 ,  G06Q30/0207 ,  G06Q30/0224 ,  G06Q30/0234 ,  G06Q30/0236 ,  G06Q30/0633 ,  G06Q30/0641 ,  G06Q40/12

Abstract: A method and system for facilitating a transaction between at least one user, at least one merchant, at least one associate is provided. The method includes the steps of the associate processing device transferring into the user processing device a first merchant link for a first merchant Web site on a first merchant processing device. The first merchant link is framed with associate information. The user selects the first merchant link. The associate processing device transfers into the user processing device information regarding a first product from the first merchant Web site. The user selects the first product to purchase. The user provides purchase information for purchasing the first product. The user processing device transfers the purchase information to the associate processing device. The associate processing device adds promotional information to the purchase information responsive to the user identification value. The associate processing device transfers the promotional information and the purchase information to the merchant processing device.

公开(公告)号：US20070026461A1

公开(公告)日：2007-02-01

申请号：US11484485

申请日：2006-07-11

Applicant: Michael Comb ,  Yi Tan ,  Patrick Li ,  Peter Hornbeck

Inventor： Michael Comb ,  Yi Tan ,  Patrick Li ,  Peter Hornbeck

IPC: C40B30/06 ,  C40B50/06 ,  C40B40/10

CPC classification number: C07K16/18 ,  C07K16/00 ,  C07K16/44 ,  C07K2317/20

Abstract: A method is provided for producing motif-specific, context-independent antibodies that recognize a plurality of peptides or proteins within a genome that contain the same post-translationally modified motif. The method includes the step of immunizing a host with a degenerate peptide library antigen featuring (i) a fixed target motif containing one or more invariant amino acids including at least one modified amino acid, and (ii) a plurality of degenerate amino acids flanking the motif. Motif-specific, context-independent antibodies produced by the disclosed method are also provided. The method encompasses motifs consisting of a single modified amino acid, as well as short motifs comprising multiple invariant amino acids including one or more modified amino acids, such as all or part of kinase consensus substrate motifs, protein-protein binding motifs, or other cell signaling motifs. Methods of using the antibodies, e.g. for genome-wide profiling, are also provided.

Abstract translation: 提供了一种用于产生基序特异性，背景无关抗体的方法，其识别基因组内含有相同翻译后修饰基序的多个肽或蛋白质。 该方法包括用简并肽文库抗原免疫宿主的步骤，其特征在于（i）含有一个或多个包含至少一个修饰的氨基酸的不变氨基酸的固定靶基序，和（ii）多个位于 主题。 还提供了通过公开的方法产生的基于特异性的，与环境无关的抗体。 该方法包括由单个修饰的氨基酸组成的基序，以及包含多个不变氨基酸的短基序，包括一个或多个修饰的氨基酸，例如全部或部分激酶共有底物基序，蛋白质 - 蛋白质结合基序或其他细胞 信号图案。 使用抗体的方法，例如 也提供了全基因组图谱。

公开(公告)号：US20060080170A1

公开(公告)日：2006-04-13

申请号：US10964164

申请日：2004-10-13

Applicant: Stan Roach ,  Michael Comb ,  Robert Drescher ,  Armen Najarian ,  Juan Velten

Inventor： Stan Roach ,  Michael Comb ,  Robert Drescher ,  Armen Najarian ,  Juan Velten

IPC: G06F17/60

CPC classification number: G06Q20/387 ,  G06Q20/06 ,  G06Q30/02 ,  G06Q30/0224 ,  G06Q30/0225 ,  G06Q30/0271

Abstract: A method, system and article of manufacture, including software, that encourages purchases of products or services and identifies measurable loyalty of an individual to a product or service, or in particular how often an individual purchases a product or service during a period of time in response to electronic media, such as a Web advertisement. The method includes providing an electronic media, such as a web banner, paid search results or e-mail to an individual that selects the electronic media and inputs individual information to a server, promotional processing device or Web site. In an embodiment, a software component to identify the individual, such as a cookie, is downloaded to an individual's processing device. In an alternate embodiment, a user identification number generated during a user session is used to identify the individual. A token, such as a first unique code associated with the individual information, is then provided to the individual's account at the server. The individual obtains a second or more tokens from one or more purchases of products at an offline and/or online retailer. The second or more tokens are then input, at the individual's processing device that transfers the information to the server, and in particular the individual's account. An object, such as downloadable music, a gift certificate, incentive, digitally downloadable asset or book, is then provided to the individual in response to the individual obtaining the required number of tokens by purchasing offline products or services.

Abstract translation: 一种方法，系统和制品，包括鼓励购买产品或服务的软件，并确定个人对产品或服务的可衡量的忠诚度，特别是个人在一段时间内购买产品或服务的频率 回应电子媒体，如网络广告。 该方法包括向选择电子媒体并将个人信息输入到服务器，促销处理设备或网站的个人提供诸如网络横幅的电子媒体，付费搜索结果或电子邮件。 在一个实施例中，将个体（例如，cookie）识别的软件组件下载到个人的处理设备。 在替代实施例中，在用户会话期间生成的用户标识号用于标识个人。 然后，将令牌（例如与个人信息相关联的第一唯一代码）提供给服务器处的个人帐户。 个人从离线和/或在线零售商的一个或多个购买产品获得第二个或更多个令牌。 然后在个人的处理设备输入第二个或更多个令牌，将信息传送到服务器，特别是个人的帐户。 然后，通过购买离线产品或服务，通过个人获得所需数量的令牌，向个人提供诸如可下载音乐，礼品券，奖励，数字可下载资产或书籍的对象。

公开(公告)号：US08332277B2

公开(公告)日：2012-12-11

申请号：US12406527

申请日：2009-03-18

Applicant: Michael McCaffery ,  Mark Bunger ,  Michael Reiling ,  Michael Comb ,  Edward Bank ,  Carolee A. Reiling ,  Juan Carlos Velten

Inventor： Michael McCaffery ,  Mark Bunger ,  Michael Reiling ,  Michael Comb ,  Edward Bank ,  Carolee A. Reiling ,  Juan Carlos Velten

IPC: G06Q30/00

CPC classification number: G06Q30/02 ,  G06Q20/20 ,  G06Q20/201 ,  G06Q20/202 ,  G06Q30/0207 ,  G06Q30/0224 ,  G06Q30/0234 ,  G06Q30/0236 ,  G06Q30/0633 ,  G06Q30/0641 ,  G06Q40/12

Abstract: A method and system for facilitating a transaction between at least one user, at least one merchant, at least one associate is provided. The method includes the steps of the associate processing device transferring into the user processing device a first merchant link for a first merchant Web site on a first merchant processing device. The first merchant link is framed with associate information. The user selects the first merchant link. The associate processing device transfers into the user processing device information regarding a first product from the first merchant Web site. The user selects the first product to purchase. The user provides purchase information for purchasing the first product. The user processing device transfers the purchase information to the associate processing device. The associate processing device adds promotional information to the purchase information responsive to the user identification value. The associate processing device transfers the promotional information and the purchase information to the merchant processing device.

Abstract translation: 提供了一种用于促进至少一个用户，至少一个商家，至少一个联系人之间的交易的方法和系统。 所述方法包括以下步骤：所述关联处理装置向所述用户处理装置传送第一商户处理装置上的第一商户网站的第一商家链接。 第一个商家链接框架有关联信息。 用户选择第一个商家链接。 关联处理装置从第一商户网站向用户处理装置传送关于第一商品的信息。 用户选择要购买的第一个产品。 用户提供购买第一个产品的购买信息。 用户处理装置将购买信息传送到关联处理装置。 辅助处理设备响应于用户识别值将促销信息添加到购买信息。 关联处理装置将促销信息和购买信息传送到商家处理装置。

公开(公告)号：US20050003450A1

公开(公告)日：2005-01-06

申请号：US10777893

申请日：2004-02-12

Applicant: John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael Comb ,  Yi Tan

Inventor： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael Comb ,  Yi Tan

IPC: C12P21/06 ,  C07K1/22 ,  C07K14/47 ,  C07K16/18 ,  C07K16/44 ,  C12P21/08 ,  C12Q1/25 ,  C12Q1/48 ,  G01N33/68 ,  G01N33/53

CPC classification number: G01N33/6872 ,  C07K14/47 ,  C07K16/44 ,  C12Q1/25 ,  C12Q1/485 ,  G01N33/6842

Abstract: The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

Abstract translation: 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS 3分析表征步骤（c）中分离的修饰肽，或步骤 （e）使用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

公开(公告)号：US09249231B2

公开(公告)日：2016-02-02

申请号：US13289651

申请日：2011-11-04

Applicant: Michael Comb ,  Ailan Guo ,  John Edward Rush, II ,  Jun-Ming Cai ,  Jing Li ,  Jing Zhou

Inventor： Michael Comb ,  Ailan Guo ,  John Edward Rush, II ,  Jun-Ming Cai ,  Jing Li ,  Jing Zhou

IPC: C07K16/44 ,  C07K16/00 ,  C12N5/07

CPC classification number: C07K16/18 ,  C07K16/44 ,  C07K2317/14 ,  C07K2317/20 ,  C07K2317/33 ,  C07K2317/34

Abstract: There is provided a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) at least one sumoylated lysine residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said sumoylated lysine residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs. Also provided is a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) a C-terminal aspartic acid residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said C-terminal aspartic acid residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs.

Abstract translation: 提供了一种特异性基因，独立于上下文的抗体，其特异性结合由（i）至少一个sumoylated赖氨酸残基组成的复发性修饰基序，和（ii）通过肽键与所述sumoylated结合的一个或多个简并氨基酸 赖氨酸残基，所述抗体特异性结合所述基序在其再次发生的生物体内的多个非同源肽或蛋白质中。 还提供了一种特异性基因，独立于上下文的抗体，其特异性结合由（i）C末端天冬氨酸残基组成的重复修饰基序，和（ii）一个或多个与肽键结合的简并氨基酸与所述 C末端天冬氨酸残基，所述抗体特异性结合所述基序在其再次发生的生物体内的多个非同源肽或蛋白质中。

公开(公告)号：US08321291B2

公开(公告)日：2012-11-27

申请号：US12485634

申请日：2009-06-16

Applicant: Michael McCaffery ,  Mark Bunger ,  Michael Reiling ,  Michael Comb ,  Edward Bank ,  Carolee A. Reiling ,  Juan Carlos Velten

Inventor： Michael McCaffery ,  Mark Bunger ,  Michael Reiling ,  Michael Comb ,  Edward Bank ,  Carolee A. Reiling ,  Juan Carlos Velten

IPC: G06Q30/00

CPC classification number: G06Q30/02 ,  G06Q20/20 ,  G06Q20/201 ,  G06Q20/202 ,  G06Q30/0207 ,  G06Q30/0224 ,  G06Q30/0234 ,  G06Q30/0236 ,  G06Q30/0633 ,  G06Q30/0641 ,  G06Q40/12

Abstract: A method and system for facilitating a transaction between at least one user, at least one merchant, at least one associate is provided. The method includes the steps of the associate processing device transferring into the user processing device a first merchant link for a first merchant Web site on a first merchant processing device. The first merchant link is framed with associate information. The user selects the first merchant link. The associate processing device transfers into the user processing device information regarding a first product from the first merchant Web site. The user selects the first product to purchase. The user provides purchase information for purchasing the first product. The user processing device transfers the purchase information to the associate processing device. The associate processing device adds promotional information to the purchase information responsive to the user identification value. The associate processing device transfers the promotional information and the purchase information to the merchant processing device.

公开(公告)号：US20120208985A1

公开(公告)日：2012-08-16

申请号：US13289651

申请日：2011-11-04

Applicant: Michael Comb ,  Ailan Guo ,  John Edward Rush, II ,  Jun-Ming Cai ,  Jing Li ,  Jing Zhou

Inventor： Michael Comb ,  Ailan Guo ,  John Edward Rush, II ,  Jun-Ming Cai ,  Jing Li ,  Jing Zhou

IPC: C07K16/00

CPC classification number: C07K16/18 ,  C07K16/44 ,  C07K2317/14 ,  C07K2317/20 ,  C07K2317/33 ,  C07K2317/34

Abstract: There is provided a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) at least one sumoylated lysine residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said sumoylated lysine residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs. Also provided is a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) a C-terminal aspartic acid residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said C-terminal aspartic acid residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs.

Abstract translation: 提供了一种特异性基因，独立于上下文的抗体，其特异性结合由（i）至少一个sumoylated赖氨酸残基组成的复发性修饰基序，和（ii）通过肽键与所述sumoylated结合的一个或多个简并氨基酸 赖氨酸残基，所述抗体特异性结合所述基序在其再次发生的生物体内的多个非同源肽或蛋白质中。 还提供了一种特异性基因，独立于上下文的抗体，其特异性结合由（i）C末端天冬氨酸残基组成的重复修饰基序，和（ii）一个或多个与肽键结合的简并氨基酸与所述 C末端天冬氨酸残基，所述抗体特异性结合所述基序在复制的生物体内的多个非同源肽或蛋白质。

公开(公告)号：US20090228341A1

公开(公告)日：2009-09-10

申请号：US12406527

申请日：2009-03-18

Applicant: Mark Bunger ,  Michael Reiling ,  Michael Comb ,  Edward Bank ,  Michael McCaffery ,  Carolee A. Reiling ,  Juan Carlos Velten

Inventor： Mark Bunger ,  Michael Reiling ,  Michael Comb ,  Edward Bank ,  Michael McCaffery ,  Carolee A. Reiling ,  Juan Carlos Velten

IPC: G06Q30/00 ,  G06Q10/00 ,  G06F3/048 ,  G06Q50/00

CPC classification number: G06Q30/02 ,  G06Q20/20 ,  G06Q20/201 ,  G06Q20/202 ,  G06Q30/0207 ,  G06Q30/0224 ,  G06Q30/0234 ,  G06Q30/0236 ,  G06Q30/0633 ,  G06Q30/0641 ,  G06Q40/12

Abstract: A method and system for facilitating a transaction between at least one user, at least one merchant, at least one associate is provided. The method includes the steps of the associate processing device transferring into the user processing device a first merchant link for a first merchant Web site on a first merchant processing device. The first merchant link is framed with associate information. The user selects the first merchant link. The associate processing device transfers into the user processing device information regarding a first product from the first merchant Web site. The user selects the first product to purchase. The user provides purchase information for purchasing the first product. The user processing device transfers the purchase information to the associate processing device. The associate processing device adds promotional information to the purchase information responsive to the user identification value. The associate processing device transfers the promotional information and the purchase information to the merchant processing device.

Abstract translation: 提供了一种用于促进至少一个用户，至少一个商家，至少一个联系人之间的交易的方法和系统。 所述方法包括以下步骤：所述关联处理装置向所述用户处理装置传送第一商户处理装置上的第一商户网站的第一商家链接。 第一个商家链接框架有关联信息。 用户选择第一个商家链接。 关联处理装置从第一商户网站向用户处理装置传送关于第一商品的信息。 用户选择要购买的第一个产品。 用户提供购买第一个产品的购买信息。 用户处理装置将购买信息传送到关联处理装置。 辅助处理设备响应于用户识别值将促销信息添加到购买信息。 关联处理装置将促销信息和购买信息传送到商家处理装置。