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公开(公告)号：US20050223421A1
公开(公告)日：2005-10-06
申请号：US10814858
申请日：2004-03-31
Applicant: Rakesh Tuli , Sawant Vishwanath , Chandra Chaturvedi , Kanti Kiran , Rajesh Mehrotra
Inventor： Rakesh Tuli , Sawant Vishwanath , Chandra Chaturvedi , Kanti Kiran , Rajesh Mehrotra
CPC classification number: C12N15/8216
Abstract: A bidirectional module for activation of gene expression and regulation of transcription in both directions is disclosed. The bidirectional module comprises multiple cis regulatory DNA sequence elements, strategically arranged to give a ‘Transcription Activating Module’ that achieves high level expression from a ‘Transcription Initiation Module’. The latter functions like a minimal promoter. The former activates transcription simultaneously in both the directions from the latter and also responds to several transcription inducing, external stimuli in both the directions. Since it is an artificially designed bidirectional transcription module, it has no equivalent DNA sequence in plant genome. This reduces the chances of the genes from being silenced by homology based mechanisms. A bidirectional promoter module as this, can therefore be used to develop efficient vectors for genetic engineering in plants.
Abstract translation: 公开了用于激活基因表达和双向转录调节的双向模块。 双向模块包括多个顺式调节DNA序列元件，策略性地排列以提供从“转录起始模块”获得高水平表达的“转录激活模块”。 后者的功能就像一个最小的启动子。 前者从后者两个方向同时激活转录，并且对两个方向的几种转录诱导，外部刺激也作出反应。 由于它是人工设计的双向转录模块，因此在植物基因组中没有等效的DNA序列。 这减少了通过基于同源性的机制使基因沉默的机会。 因此，双向启动子模块因此可用于开发植物中遗传工程的有效载体。
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公开(公告)号：US07390661B2
公开(公告)日：2008-06-24
申请号：US11542590
申请日：2006-10-03
Applicant: Rakesh Tuli , Sawant Samir Vishwanath , Chandra Prakash Chaturvedi , Kanti Kiran , Rajesh Mehrotra
Inventor： Rakesh Tuli , Sawant Samir Vishwanath , Chandra Prakash Chaturvedi , Kanti Kiran , Rajesh Mehrotra
CPC classification number: C12N15/8216
Abstract: A bidirectional module for activation of gene expression and regulation of transcription in both directions is disclosed. The bidirectional module comprises multiple cis regulatory DNA sequence elements, strategically arranged to give a ‘Transcription Activating Module’ that achieves high level expression from a ‘Transcription Initiation Module’. The latter functions like a minimal promoter. The former activates transcription simultaneously in both the directions from the latter and also responds to several transcription inducing, external stimuli in both the directions. Since it is an artificially designed bidirectional transcription module, it has no equivalent DNA sequence in plant genome. This reduces the chances of the genes from being silenced by homology based mechanisms. A bidirectional promoter module as this, can therefore be used to develop efficient vectors for genetic engineering in plants.
Abstract translation: 公开了用于激活基因表达和双向转录调节的双向模块。 双向模块包括多个顺式调节DNA序列元件，策略性地排列成一个“转录激活模块”，从“转录起始模块”获得高水平的表达。 后者的功能就像一个最小的启动子。 前者从后者两个方向同时激活转录，并且对两个方向的几种转录诱导，外部刺激也作出反应。 由于它是人工设计的双向转录模块，因此在植物基因组中没有等效的DNA序列。 这减少了通过基于同源性的机制使基因沉默的机会。 因此，双向启动子模块因此可用于开发植物中遗传工程的有效载体。
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公开(公告)号：US20070028323A1
公开(公告)日：2007-02-01
申请号：US11542590
申请日：2006-10-03
Applicant: Rakesh Tuli , Sawant Vishwanath , Chandra Chaturvedi , Kanti Kiran , Rajesh Mehrotra
Inventor： Rakesh Tuli , Sawant Vishwanath , Chandra Chaturvedi , Kanti Kiran , Rajesh Mehrotra
CPC classification number: C12N15/8216
Abstract: A bidirectional module for activation of gene expression and regulation of transcription in both directions is disclosed. The bidirectional module comprises multiple cis regulatory DNA sequence elements, strategically arranged to give a ‘Transcription Activating Module’ that achieves high level expression from a ‘Transcription Initiation Module’. The latter functions like a minimal promoter. The former activates transcription simultaneously in both the directions from the latter and also responds to several transcription inducing, external stimuli in both the directions. Since it is an artificially designed bidirectional transcription module, it has no equivalent DNA sequence in plant genome. This reduces the chances of the genes from being silenced by homology based mechanisms. A bidirectional promoter module as this, can therefore be used to develop efficient vectors for genetic engineering in plants.
Abstract translation: 公开了用于激活基因表达和双向转录调节的双向模块。 双向模块包括多个顺式调节DNA序列元件，策略性地排列以提供从“转录起始模块”获得高水平表达的“转录激活模块”。 后者的功能就像一个最小的启动子。 前者从后者两个方向同时激活转录，并且对两个方向的几种转录诱导，外部刺激也作出反应。 由于它是人工设计的双向转录模块，因此在植物基因组中没有等效的DNA序列。 这减少了通过基于同源性的机制使基因沉默的机会。 因此，双向启动子模块因此可用于开发植物中遗传工程的有效载体。
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公开(公告)号：US07235652B2
公开(公告)日：2007-06-26
申请号：US10814858
申请日：2004-03-31
Applicant: Rakesh Tuli , Sawant Samir Vishwanath , Chandra Prakash Chaturvedi , Kanti Kiran , Rajesh Mehrotra
Inventor： Rakesh Tuli , Sawant Samir Vishwanath , Chandra Prakash Chaturvedi , Kanti Kiran , Rajesh Mehrotra
CPC classification number: C12N15/8216
Abstract: A bidirectional module for activation of gene expression and regulation of transcription in both directions is disclosed. The bidirectional module comprises multiple cis regulatory DNA sequence elements, strategically arranged to give a ‘Transcription Activating Module’ that achieves high level expression from a ‘Transcription Initiation Module’. The latter functions like a minimal promoter. The former activates transcription simultaneously in both the directions from the latter and also responds to several transcription inducing, external stimuli in both the directions. Since it is an artificially designed bidirectional transcription module, it has no equivalent DNA sequence in plant genome. This reduces the chances of the genes from being silenced by homology based mechanisms. A bidirectional promoter module as this, can therefore be used to develop efficient vectors for genetic engineering in plants.
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