公开(公告)号：US20130144073A1

公开(公告)日：2013-06-06

申请号：US13635642

申请日：2011-03-17

Applicant: Max M. Tirtowidjojo ,  Subrata Sen ,  Christina Zarth ,  Pramod D. Patil

Inventor： Max M. Tirtowidjojo ,  Subrata Sen ,  Christina Zarth ,  Pramod D. Patil

IPC: B01J19/24

CPC classification number: B01J19/24 ,  B01J8/0242 ,  B01J8/0264 ,  B01J8/0285 ,  B01J2208/00115 ,  B01J2208/00141 ,  B01J2208/00557 ,  B01J2208/00893 ,  B01J2208/02 ,  C07D301/08 ,  C07D301/10

Abstract: At least one method to efficiently produce alkylene oxide from partial oxidation of hydrocarbons using a high efficiency heterogeneous catalyst in a fixed bed enclosed within a reaction vessel, and a reaction vessel constructed to facilitate the same.

公开(公告)号：US09073034B2

公开(公告)日：2015-07-07

申请号：US13635642

申请日：2011-03-17

Applicant: Max M. Tirtowidjojo ,  Subrata Sen ,  Christina Zarth ,  Pramod D. Patil

Inventor： Max M. Tirtowidjojo ,  Subrata Sen ,  Christina Zarth ,  Pramod D. Patil

IPC: C07D301/03 ,  F28D7/00 ,  B01J19/24 ,  B01J8/02 ,  C07D301/10

CPC classification number: B01J19/24 ,  B01J8/0242 ,  B01J8/0264 ,  B01J8/0285 ,  B01J2208/00115 ,  B01J2208/00141 ,  B01J2208/00557 ,  B01J2208/00893 ,  B01J2208/02 ,  C07D301/08 ,  C07D301/10

Abstract: At least one method to efficiently produce alkylene oxide from partial oxidation of hydrocarbons using a high efficiency heterogeneous catalyst in a fixed bed enclosed within a reaction vessel, and a reaction vessel constructed to facilitate the same.

Abstract translation: 在封闭在反应容器内的固定床中使用高效多相催化剂，有效地从烃的部分氧化产生环氧烷烃的至少一种方法，以及为促进烯烃氧化反应构成的反应容器。

公开(公告)号：US20130288909A1

公开(公告)日：2013-10-31

申请号：US13819898

申请日：2011-08-31

Applicant: Subrata Sen ,  Jin Wang

Inventor： Subrata Sen ,  Jin Wang

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/178

Abstract: Methods for the diagnosis and/or monitoring of pancreatic cancer by detection of micro-RNAs (miRNA) are provided. In certain embodiments, detection of miR-21, miR-210, miR-155, and/or miR-196a in the plasma, blood, or pancreatic juice of a subject, such as a human patient, may be used to detect, diagnose, or monitor a pancreatic cancer.

Abstract translation: 提供了通过检测微RNA（miRNA）来诊断和/或监测胰腺癌的方法。 在某些实施方案中，可以使用受试者例如人类患者的血浆，血液或胰液中的miR-21，miR-210，miR-155和/或miR-196a的检测，诊断 ，或监测胰腺癌。

公开(公告)号：US20080182295A1

公开(公告)日：2008-07-31

申请号：US12013042

申请日：2008-01-11

Applicant: Anant Y. Patkar ,  Subrata Sen ,  Micheal L. Chappell

Inventor： Anant Y. Patkar ,  Subrata Sen ,  Micheal L. Chappell

IPC: C12P21/04 ,  C12M1/00 ,  C12N5/06 ,  C12N5/04 ,  C12N1/20 ,  C12N1/00

CPC classification number: C12P21/02 ,  C12M47/10 ,  C12N1/005 ,  C12N1/06 ,  C12N15/78

Abstract: A method of preparing a recombinant polypeptide of interest includes fermenting a host cell being transformed with a recombinant expression system capable of bringing about secretion of a polypeptide of interest into the periplasm of said host cell. The polypeptide of interest is extracted from the periplasm by applying a continuous osmotic shock to the host cells contained in a fermentation medium. An apparatus for osmotically shocking cells includes a first reservoir containing cells in a first solution and a second reservoir containing a second solution, the first solution having a higher osmolarity than the second solution. A method for osmotically shocking cells using the first and second solutions is also disclosed. Also disclosed is a method of isolating a recombinant polypeptide of interest from a cell.

Abstract translation: 制备感兴趣的重组多肽的方法包括用能够使感兴趣的多肽分泌到所述宿主细胞的周质中的重组表达系统来发酵正在转化的宿主细胞。 通过对包含在发酵培养基中的宿主细胞进行连续渗透休克，从周质中提取目的多肽。 一种用于渗透电击的细胞的装置包括在第一溶液中含有细胞的第一储存器和含有第二溶液的第二储存器，所述第一溶液具有比第二溶液更高的渗透压浓度。 还公开了使用第一和第二溶液的渗透电击细胞的方法。 还公开了从细胞分离目的重组多肽的方法。

公开(公告)号：US08211668B2

公开(公告)日：2012-07-03

申请号：US12013042

申请日：2008-01-11

Applicant: Anant Y. Patkar ,  Subrata Sen ,  Micheal L. Chappell

Inventor： Anant Y. Patkar ,  Subrata Sen ,  Micheal L. Chappell

IPC: C12P21/04 ,  C12M1/00 ,  C12N5/04

CPC classification number: C12P21/02 ,  C12M47/10 ,  C12N1/005 ,  C12N1/06 ,  C12N15/78

Abstract: A method of preparing a recombinant polypeptide of interest includes fermenting a host cell being transformed with a recombinant expression system capable of bringing about secretion of a polypeptide of interest into the periplasm of said host cell. The polypeptide of interest is extracted from the periplasm by applying a continuous osmotic shock to the host cells contained in a fermentation medium. An apparatus for osmotically shocking cells includes a first reservoir containing cells in a first solution and a second reservoir containing a second solution, the first solution having a higher osmolarity than the second solution. A method for osmotically shocking cells using the first and second solutions is also disclosed. Also disclosed is a method of isolating a recombinant polypeptide of interest from a cell.

Abstract translation: 制备感兴趣的重组多肽的方法包括用能够使感兴趣的多肽分泌到所述宿主细胞的周质中的重组表达系统来发酵正在转化的宿主细胞。 通过对包含在发酵培养基中的宿主细胞进行连续渗透休克，从周质中提取目的多肽。 一种用于渗透电击的细胞的装置包括在第一溶液中含有细胞的第一储存器和含有第二溶液的第二储存器，所述第一溶液具有比第二溶液更高的渗透压浓度。 还公开了使用第一和第二溶液的渗透电击细胞的方法。 还公开了从细胞分离目的重组多肽的方法。