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    Biosynthetic gene cluster for leptomycins
    3.
    发明申请
    Biosynthetic gene cluster for leptomycins 有权
    leptomycins的生物合成基因簇

    公开(公告)号：US20080233618A1

    公开(公告)日：2008-09-25

    申请号：US11895327

    申请日：2007-08-24

    Applicant: Zhihao Hu ,  Ralph Reid

    Inventor： Zhihao Hu ,  Ralph Reid

    IPC: C12P17/06 ,  C12N9/10 ,  C12N1/00 ,  C12N15/54 ,  C12N1/21

    CPC classification number: C12N15/52 ,  C07K2319/00

    Abstract: Polypeptides and domains of leptomycin polyketide synthase and the nucleic acids encoding them are provided. Methods to prepare leptomycin, leptomycin analogs, and leptomycin derivatives are described, as are methods to prepare other polyketides using the nucleic acids encoding leptomycin polyketide synthase domains or modifying enzymes.

    Abstract translation: 提供了多肽和克隆霉素聚酮合成酶的结构域和编码它们的核酸。 还描述了制备雷霉霉素，曲霉素类似物和曲霉素衍生物的方法，以及使用编码雷霉霉素聚酮合成酶结构域或修饰酶的核酸制备其它聚酮化合物的方法。

    Plasmids for polyketide production
    4.
    发明申请
    Plasmids for polyketide production 审中-公开
    用于聚酮化合物生产的质粒

    公开(公告)号：US20050208629A1

    公开(公告)日：2005-09-22

    申请号：US11050937

    申请日：2005-02-04

    Applicant: Zhihao Hu ,  C. Hutchinson

    Inventor： Zhihao Hu ,  C. Hutchinson

    IPC: C12N1/21 ,  C12N9/00 ,  C12N15/52 ,  C12N15/74 ,  C12P19/62

    CPC classification number: C12N15/52 ,  C12P19/62

    Abstract: Streptomyces plasmids comprising a SCP2*-derived vector, lacking a specific 45 base pair sequence, have a higher copy number and can be used to increase gene expression in host cells. Such plasmids, encoding polyketide synthase genes, are useful for polyketide production in host cells.

    Abstract translation: 包含SCP2 *衍生载体（缺乏特异性45碱基对序列）的链霉菌质粒具有较高的拷贝数，可用于增加宿主细胞中的基因表达。 编码聚酮化合物合酶基因的这种质粒可用于宿主细胞中聚酮化合物的产生。

    Methods and Compositions for Identification of Hydrocarbon Response, Transport and Biosynthesis Genes
    10.
    发明申请
    Methods and Compositions for Identification of Hydrocarbon Response, Transport and Biosynthesis Genes 审中-公开
    用于鉴定烃反应，运输和生物合成基因的方法和组合物

    公开(公告)号：US20080293060A1

    公开(公告)日：2008-11-27

    申请号：US12108278

    申请日：2008-04-23

    Applicant: Andreas Schirmer ,  Zhihao Hu ,  Bernardo da Costa

    Inventor： Andreas Schirmer ,  Zhihao Hu ,  Bernardo da Costa

    IPC: C12Q1/68 ,  C12N1/21

    CPC classification number: C12Q1/6897 ,  C12N15/1086

    Abstract: Disclosed is a method using an alkane response element (ARE) from, e.g., Acinetobacter spp. to (i) identify and clone hydrocarbon biosynthesis genes, (ii) identify and clone hydrocarbon transporter genes (iii) identify and clone hydrocarbon response genes. Screening cells were developed that expressed a transcriptional activator, e.g., alkR, and included a reporter gene, e.g., GFP operatively linked to an ARE promoter, e.g., the alkM promoter. The cells were transformed with libraries from organisms capable of hydrocarbon biosynthesis. Transformed cells that expressed the reporter gene harbored library-derived genes involved in one or more of the above-mentioned processes; and these genes were isolated from the cells using standard molecular biology techniques. Additional systems were designed wherein screening cells also expressed a gene identified in the original screen, e.g., an additional hydrocarbon pathway gene, e.g., an enhancer.

    Abstract translation: 公开了一种使用来自例如不动杆菌属（Acinetobacter spp）的烷烃应答元件（ARE）的方法。 （i）识别和克隆烃类生物合成基因，（ii）鉴定和克隆烃转运蛋白基因（iii）鉴定和克隆烃类反应基因。 开发了表达转录激活物例如alkR的筛选细胞，并且包括报告基因，例如可操作地连接到ARE启动子的例如alkM启动子的GFP。 用能够进行烃生物合成的生物体的文库转化细胞。 表达报告基因的转化细胞携带参与一个或多个上述过程的文库衍生基因; 并使用标准分子生物学技术从细胞中分离出这些基因。 设计另外的系统，其中筛选细胞还表达在原始筛选中鉴定的基因，例如另外的烃途径基因，例如增强子。

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