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    Sequence conversion and signal amplifier DNA having locked nucleic acids and detection methods using same
    1.
    发明授权
    Sequence conversion and signal amplifier DNA having locked nucleic acids and detection methods using same 有权

    公开(公告)号：US10208333B2

    公开(公告)日：2019-02-19

    申请号：US14882124

    申请日：2015-10-13

    Applicant: ABBOTT LABORATORIES ,  TOKYO INSTITUTE OF TECHNOLOGY

    Inventor： Ken Komiya ,  Makoto Komori ,  Toru Yoshimura

    IPC: C12Q1/68 ,  C12Q1/682 ,  C12Q1/6844

    Abstract: Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting said sample, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide having locked nucleic acids at select positions sufficient to decrease non-specific background signal amplification. Also disclosed are methods for detecting a target nucleic acid in a sample in which said sample is contacted, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide and a signal amplifier oligonucleotide, both having locked nucleic acids at select positions sufficient to decrease non-specific background signal amplification. The disclosure also provides compositions and kits comprising such sequence conversion and signal amplifier oligonucleotides.

    SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA HAVING ABASIC NUCLEIC ACIDS, AND DETECTION METHODS USING SAME
    5.
    发明公开
    SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA HAVING ABASIC NUCLEIC ACIDS, AND DETECTION METHODS USING SAME 审中-公开

    公开(公告)号：US20240117414A1

    公开(公告)日：2024-04-11

    申请号：US18362013

    申请日：2023-07-31

    Applicant: ABBOTT LABORATORIES

    Inventor： Takamitsu Morikawa ,  Toru Yoshimura

    IPC: C12Q1/6816

    CPC classification number: C12Q1/6816 ,  C12Q2600/166

    Abstract: Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting the sample, in the presence of a polymerase and an endonuclease, with a first oligonucleotide that includes, in the 5′ to 3′ direction, a signal DNA generation sequence, an endonuclease recognition site, and a complementary sequence that has at least one abasic moiety and wherein the complementary sequence has a first complementary sequence that is complementary to at least a portion of the signal DNA generation sequence and a second complementary sequence that is complementary to the 3′ end of the target nucleic acid. Also disclosed are methods that include a second oligonucleotide including, in the 5′ to 3′ direction, a second signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the first signal DNA generation sequence of the first oligonucleotide and that optionally has at least one abasic site. Also disclosed chemically modified oligonucleotides, as well as compositions and kits that include the chemically modified oligonucleotides for detecting a target nucleic acid.

    IMMUNOASSAY METHOD FOR PRO-GASTRIN-RELEASING PEPTIDE
    6.
    发明申请
    IMMUNOASSAY METHOD FOR PRO-GASTRIN-RELEASING PEPTIDE 审中-公开
    免疫抑制肽的免疫测定方法

    公开(公告)号：US20140287532A1

    公开(公告)日：2014-09-25

    申请号：US14295116

    申请日：2014-06-03

    Applicant: Abbott Laboratories

    Inventor： Toru Yoshimura ,  Kenju Fujita ,  Barry L. Dowell

    IPC: G01N33/53

    CPC classification number: G01N33/5306 ,  G01N33/57423 ,  G01N33/86 ,  G01N2333/5758

    Abstract: To provide a more convenient and more accurate method of assaying ProGRP by improving the stability of ProGRP which is known to be unstable in a biological sample.By using a blood sample in a condition in which a blood coagulation factor is not activated is used as a sample, the degradation of ProGRP is suppressed, whereby it is possible to store a sample for a long period of time and to improve the accuracy of an assay.

    Abstract translation: 通过提高已知在生物样品中不稳定的ProGRP的稳定性来提供更方便和更准确的测定ProGRP的方法。 通过使用血液凝固因子未活化的状态下的血液样品作为样品，可以抑制ProGRP的降解，能够长时间存储样品，提高准确度 一个测定。

    Systems and Methods for Sample Analysis
    7.
    发明申请
    Systems and Methods for Sample Analysis 有权

    公开(公告)号：US20250001410A1

    公开(公告)日：2025-01-02

    申请号：US18705941

    申请日：2022-11-03

    Applicant: Abbott Laboratories

    Inventor： Toru Yoshimura ,  Yoshiyuki Arai ,  Tomotaka Komori ,  Ryotaro Chiba ,  Jeffrey B. Huff

    IPC: B01L3/00

    Abstract: Sample analysis systems and methods using assay surfaces, assay processing units (APUs), assay processing systems (APSs), and laboratory systems are disclosed. An assay surface includes a sample processing component comprising a plurality of regions, including at least one wash region and at least one storage region configured to hold a plurality of solid supports moveable through the regions under a magnetic force, and a detection component configured to receive the solid supports. An APU includes an assay surface receiving component, a magnetic element configured to generate a moveable magnetic field, and one or more processors configured to move the magnetic field. An APS includes one or more assay surfaces and an APU. A laboratory system includes one or more APSs and a controller for parallel processing. Sample processing and detection methods are disclosed with a reduced sample volume and/or shortened processing time and/or higher sensitivity.

    Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same
    8.
    发明授权
    Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same 有权

    公开(公告)号：US11492658B2

    公开(公告)日：2022-11-08

    申请号：US16816887

    申请日：2020-03-12

    Applicant: ABBOTT LABORATORIES

    Inventor： Makoto Komori ,  Toru Yoshimura

    IPC: C12Q1/682 ,  C12Q1/6844 ,  C12Q1/6886

    Abstract: Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting the sample, in the presence of a polymerase and an endonuclease, with a first oligonucleotide comprising, in the 5′ to 3′ direction, a first signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of a target nucleic acid; a second oligonucleotide comprising, in the 5′ to 3′ direction, a second signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the first signal DNA generation sequence of the first oligonucleotide; a third oligonucleotide comprising, in the 5′ to 3′ direction, a third signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the second signal DNA generation sequence of the second oligonucleotide.

    SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA CASCADE REACTIONS AND DETECTION METHODS USING SAME
    9.
    发明申请
    SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA CASCADE REACTIONS AND DETECTION METHODS USING SAME 审中-公开

    公开(公告)号：US20200283822A1

    公开(公告)日：2020-09-10

    申请号：US16816887

    申请日：2020-03-12

    Applicant: ABBOTT LABORATORIES

    Inventor： Makoto Komori ,  Toru Yoshimura

    IPC: C12Q1/682 ,  C12Q1/6844 ,  C12Q1/6886

    Abstract: Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting the sample, in the presence of a polymerase and an endonuclease, with a first oligonucleotide comprising, in the 5′ to 3′ direction, a first signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of a target nucleic acid; a second oligonucleotide comprising, in the 5′ to 3′ direction, a second signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the first signal DNA generation sequence of the first oligonucleotide; a third oligonucleotide comprising, in the 5′ to 3′ direction, a third signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the second signal DNA generation sequence of the second oligonucleotide.

    Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same
    10.
    发明授权
    Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same 有权

    公开(公告)号：US10604790B2

    公开(公告)日：2020-03-31

    申请号：US14998162

    申请日：2015-12-24

    Applicant: ABBOTT LABORATORIES

    Inventor： Makoto Komori ,  Toru Yoshimura

    IPC: C12Q1/68 ,  C12Q1/682 ,  C12Q1/6844 ,  C12Q1/6886

    Abstract: Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting the sample, in the presence of a polymerase and an endonuclease, with a first oligonucleotide comprising, in the 5′ to 3′ direction, a first signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of a target nucleic acid; a second oligonucleotide comprising, in the 5′ to 3′ direction, a second signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the first signal DNA generation sequence of the first oligonucleotide; a third oligonucleotide comprising, in the 5′ to 3′ direction, a third signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the second signal DNA generation sequence of the second oligonucleotide.

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