公开(公告)号：US20190127790A1

公开(公告)日：2019-05-02

申请号：US16020190

申请日：2018-06-27

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Brian P. STUPI ,  Hong LI ,  Weidong WU ,  Megan N. HERSH ,  David HERTZOG ,  Sidney E. MORRIS ,  Michael L. METZKER

IPC: C12Q1/6869 ,  C07H19/10 ,  C07H19/173 ,  C07H19/20 ,  C12Q1/6883 ,  C12Q1/6874 ,  B01L3/00 ,  C07H19/073 ,  C07H19/14

Abstract: The present invention relates generally to 3′-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.

公开(公告)号：US20210181191A1

公开(公告)日：2021-06-17

申请号：US17174860

申请日：2021-02-12

Applicant: Agilent Technologies, Inc.

Inventor： Mimi HEALY ,  Xiyi CHEN ,  Jinchun WANG ,  Weidong WU ,  Sophia PETRICHENKO ,  Su ZHANG ,  Stacie FRYE

IPC: G01N33/543 ,  B01L3/00 ,  G01N1/30 ,  G01N1/31 ,  G01N33/533 ,  G01N33/53 ,  G01N35/00 ,  G01N33/58

Abstract: Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.