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公开(公告)号：US20180327833A1
公开(公告)日：2018-11-15
申请号：US15979185
申请日：2018-05-14
Inventor： Craig A. Aspinwall , Phuong-Diem Nguyen , Jinyan Wang , Xuemin Wang
IPC: C12Q1/6874 , C12Q1/6809 , C12Q1/6816 , C12Q1/6832 , C12Q1/6837
CPC classification number: C12Q1/6874 , C12Q1/6809 , C12Q1/6816 , C12Q1/6832 , C12Q1/6837
Abstract: Microarray platforms and methods of fabricating said microarrays without traditional high aspect ratio barriers used to define individual array elements are described herein. Self-assembled nanoshells were stabilized with a polymerized scaffold to enhance the stability in physiological conditions and serve as an optical transducer upon molecular recognition events. Soft photolithography combined with surface chemistry was developed for covalent immobilization of nanoshells onto the pre-patterned arrayed microspots for rapid multiplexed detection of membrane-binding analytes. This robust fabrication methodology is amenable for general lipid structures, and thus facilitates the integration of stable membrane architectures into diagnostic and prognostic platforms. In particular, the microarray platform may be used in diverse applications ranging from the detection of pathogens, such bacterial toxin in biological matrices, to cellular membrane studies.
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公开(公告)号：US20210088509A1
公开(公告)日：2021-03-25
申请号：US17111037
申请日：2020-12-03
Inventor： Craig A. Aspinwall , Jinyan Wang , Kendall Sandy , Steven Scott Saavedra , Christopher Baker , Elyssia S. Gallagher , Boying Liang
IPC: G01N33/543
Abstract: Surface-modified silica microparticles that are functionalized with stabilized phospholipid vesicles are described herein. These stabilized vesicles can be functionalized with either transmembrane receptors or membrane associated receptors and used for affinity pull-down assays or other chromatographic separation modalities to provide affinity capture/concentration of low abundance ligands in complex mixtures with minimal sample preparation. Further described are methods and apparatus for forming polymer frits in a fused silica capillary. The capillary containing a monomer solution is placed between one or more heat sources connected to each other via a jig and operatively coupled to a temperature controller. The polymer frits are synthesized via thermal polymerization of the monomer solution using the heat sources, which allows for placement of the polymer frits at a spatially-defined location in the capillary.
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公开(公告)号：US20180224438A1
公开(公告)日：2018-08-09
申请号：US15751125
申请日：2016-08-09
Inventor： Craig A. Aspinwall , Jinyan Wang , Kendall Sandy , Steven Scott Saavedra , Christopher Baker , Elyssia S. Gallagher
IPC: G01N33/543 , G01N33/545 , G01N33/531 , B01L7/00 , B01L3/00
CPC classification number: G01N33/5432 , B01J20/286 , B01J20/3204 , B01J20/3272 , B01J20/3287 , B01J2220/84 , B01J2220/86 , B01L3/5082 , B01L7/00 , B01L2300/0832 , B01L2300/18 , B01L2300/1805 , C07K14/705 , C07K17/14 , G01N33/531 , G01N33/54353 , G01N33/545
Abstract: Surface-modified silica microparticles that are functionalized with stabilized phospholipid vesicles are described herein. These stabilized vesicles can be functionalized with either transmembrane receptors or membrane associated receptors and used for affinity pull-down assays or other chromatographic separation modalities to provide affinity capture/concentration of low abundance ligands in complex mixtures with minimal sample preparation. Further described are methods and apparatus for forming polymer frits in a fused silica capillary. The capillary containing a monomer solution is placed between one or more heat sources connected to each other via a jig and operatively coupled to a temperature controller. The polymer frits are synthesized via thermal polymerization of the monomer solution using the heat sources, which allows for placement of the polymer frits at a spatially-defined location in the capillary.
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