公开(公告)号：US20180224438A1

公开(公告)日：2018-08-09

申请号：US15751125

申请日：2016-08-09

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Craig A. Aspinwall ,  Jinyan Wang ,  Kendall Sandy ,  Steven Scott Saavedra ,  Christopher Baker ,  Elyssia S. Gallagher

IPC: G01N33/543 ,  G01N33/545 ,  G01N33/531 ,  B01L7/00 ,  B01L3/00

CPC classification number: G01N33/5432 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3272 ,  B01J20/3287 ,  B01J2220/84 ,  B01J2220/86 ,  B01L3/5082 ,  B01L7/00 ,  B01L2300/0832 ,  B01L2300/18 ,  B01L2300/1805 ,  C07K14/705 ,  C07K17/14 ,  G01N33/531 ,  G01N33/54353 ,  G01N33/545

Abstract: Surface-modified silica microparticles that are functionalized with stabilized phospholipid vesicles are described herein. These stabilized vesicles can be functionalized with either transmembrane receptors or membrane associated receptors and used for affinity pull-down assays or other chromatographic separation modalities to provide affinity capture/concentration of low abundance ligands in complex mixtures with minimal sample preparation. Further described are methods and apparatus for forming polymer frits in a fused silica capillary. The capillary containing a monomer solution is placed between one or more heat sources connected to each other via a jig and operatively coupled to a temperature controller. The polymer frits are synthesized via thermal polymerization of the monomer solution using the heat sources, which allows for placement of the polymer frits at a spatially-defined location in the capillary.

公开(公告)号：US20210088509A1

公开(公告)日：2021-03-25

申请号：US17111037

申请日：2020-12-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Craig A. Aspinwall ,  Jinyan Wang ,  Kendall Sandy ,  Steven Scott Saavedra ,  Christopher Baker ,  Elyssia S. Gallagher ,  Boying Liang

IPC: G01N33/543

Abstract: Surface-modified silica microparticles that are functionalized with stabilized phospholipid vesicles are described herein. These stabilized vesicles can be functionalized with either transmembrane receptors or membrane associated receptors and used for affinity pull-down assays or other chromatographic separation modalities to provide affinity capture/concentration of low abundance ligands in complex mixtures with minimal sample preparation. Further described are methods and apparatus for forming polymer frits in a fused silica capillary. The capillary containing a monomer solution is placed between one or more heat sources connected to each other via a jig and operatively coupled to a temperature controller. The polymer frits are synthesized via thermal polymerization of the monomer solution using the heat sources, which allows for placement of the polymer frits at a spatially-defined location in the capillary.