公开(公告)号：US20040259076A1

公开(公告)日：2004-12-23

申请号：US10601378

申请日：2003-06-23

Applicant: Accella Scientific, Inc.

Inventor： David Farrow

IPC: C12Q001/70

CPC classification number: B82Y30/00 ,  B01D61/18 ,  B01F3/20 ,  B01F3/2057 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/0237 ,  B01F2215/0431 ,  B01F2215/045 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2300/0896 ,  B01L2400/0478 ,  B01L2400/0481 ,  C12Q1/703 ,  G01N1/405 ,  G01N15/1031 ,  G01N33/56988 ,  C12Q2565/501

Abstract: The invention relates to methods and devices for detecting the presence of a particle of interest (hereinafter an analyte particle) in a fluid. A detection device exemplary of the present invention filters a sample of the fluid to remove particles larger than the analyte particles. A reagent solution, containing reagent particles smaller than the analyte particles, is then added to the sample. The reagent particles will react with the analyte particles, if any are present, to form reagent-analyte complexes which are larger than the analyte particles. The sample is then filtered a second time to remove particles the same size as or smaller than the analyte particles. The sample is then tested for the presence of reagent-analyte complexes to detect the presence of the analyte particle in the fluid.

Abstract translation: 本发明涉及用于检测流体中感兴趣的颗粒（以下称为分析物颗粒）的存在的方法和装置。 本发明示例性的检测装置对流体样品进行过滤以除去大于分析物颗粒的颗粒。 然后将含有小于分析物颗粒的试剂颗粒的试剂溶液加入到样品中。 试剂颗粒将与分析物颗粒（如果有的话）反应，形成大于分析物颗粒的试剂 - 分析物复合物。 然后将样品第二次过滤以除去与分析物颗粒相同的尺寸或小于分析物颗粒的颗粒。 然后对样品进行试剂 - 分析物复合物的存在，以检测流体中分析物颗粒的存在。