中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

10 records (took 0.018 seconds)

    In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
    1.
    发明授权
    In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto 有权

    公开(公告)号：US11078528B2

    公开(公告)日：2021-08-03

    申请号：US15291054

    申请日：2016-10-11

    Applicant: Advanced Cell Diagnostics, Inc.

    Inventor： Yuling Luo ,  Xingyong Wu ,  Liuliu Pan ,  Xiaoming Wang ,  Xiao-Jun Ma ,  Nan Su ,  Steve Chen

    IPC: C12Q1/68 ,  C12Q1/6841 ,  C12Q1/682 ,  C12Q1/6827

    Abstract: The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample. The invention also provides samples, tissue slides, and kits relating to detection of nucleic acid variations, including single nucleotide variations, multi-nucleotide variations or splice sites, of a target nucleic acid.

    Ultra sensitive method for in situ detection of nucleic acids
    2.
    发明授权
    Ultra sensitive method for in situ detection of nucleic acids 有权
    用于原位检测核酸的超敏感方法

    公开(公告)号：US09315854B2

    公开(公告)日：2016-04-19

    申请号：US14151716

    申请日：2014-01-09

    Applicant: Advanced Cell Diagnostics, Inc.

    Inventor： Xingyong Wu ,  Huei-Yu Fay Wang ,  Nan Su ,  Li-Chong Wang ,  Yuling Luo

    IPC: C12Q1/68 ,  C12Q1/00 ,  G01N33/53 ,  C12Q1/28 ,  C12P19/34 ,  C12M1/34 ,  G01N31/22 ,  C07H21/02 ,  C07H21/04

    CPC classification number: C12Q1/6841 ,  C12Q1/6818 ,  C12Q1/682 ,  C12Q2563/131 ,  C12Q2537/143 ,  C12Q2537/125

    Abstract: Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of an in situ hybridization (ISH) assay method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of the ISH assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.

    Abstract translation: 公开了一种基于原位杂交（ISH）测定方法和一般ISH信号扩增方法的组合原位检测一种或多种靶核酸的方法。 这种新方法产生高信号强度，同时保持低信号放大的背景噪声。 结果可以一贯重现，该方法可以方便地用于常规诊所诊断。 此外，本发明涉及一种试剂盒，其包含用于敏感检测一种或多种靶核酸的ISH测定和一般ISH信号扩增测定的组分。

    APPARATUS AND SYSTEM FOR NUCLEIC ACID DETECTION
    3.
    发明公开
    APPARATUS AND SYSTEM FOR NUCLEIC ACID DETECTION 审中-公开

    公开(公告)号：US20230143970A1

    公开(公告)日：2023-05-11

    申请号：US18046775

    申请日：2022-10-14

    Applicant: ADVANCED CELL DIAGNOSTICS, INC.

    Inventor： Shiping Chen ,  Yuling Luo

    IPC: C12Q1/6837 ,  C12Q1/682 ,  C12Q1/6841

    CPC classification number: C12Q1/6837 ,  C12Q1/682 ,  C12Q1/6841

    Abstract: Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.

    IN SITU DETECTION OF NUCLEOTIDE VARIANTS IN HIGH NOISE SAMPLES, AND COMPOSITIONS AND METHODS RELATED THERETO
    4.
    发明申请
    IN SITU DETECTION OF NUCLEOTIDE VARIANTS IN HIGH NOISE SAMPLES, AND COMPOSITIONS AND METHODS RELATED THERETO 有权

    公开(公告)号：US20210395806A1

    公开(公告)日：2021-12-23

    申请号：US17362828

    申请日：2021-06-29

    Applicant: ADVANCED CELL DIAGNOSTICS, INC.

    Inventor： Yuling Luo ,  Xingyong Wu ,  Liuliu Pan ,  Xiaoming Wang ,  Xiao-Jun Ma ,  Nan Su ,  Steve Chen

    IPC: C12Q1/6841 ,  C12Q1/682 ,  C12Q1/6827

    Abstract: The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample. The invention also provides samples, tissue slides, and kits relating to detection of nucleic acid variations, including single nucleotide variations, multi-nucleotide variations or splice sites, of a target nucleic acid.

    IN SITU DETECTION OF NUCLEOTIDE VARIANTS IN HIGH NOISE SAMPLES, AND COMPOSITIONS AND METHODS RELATED THERETO
    7.
    发明申请
    IN SITU DETECTION OF NUCLEOTIDE VARIANTS IN HIGH NOISE SAMPLES, AND COMPOSITIONS AND METHODS RELATED THERETO 有权

    公开(公告)号：US20240392355A1

    公开(公告)日：2024-11-28

    申请号：US18789872

    申请日：2024-07-31

    Applicant: ADVANCED CELL DIAGNOSTICS, INC.

    Inventor： Yuling Luo ,  Xingyong Wu ,  Liuliu Pan ,  Xiaoming Wang ,  Xiao-Jun Ma ,  Nan Su ,  Steve Chen

    IPC: C12Q1/6841 ,  C12Q1/682 ,  C12Q1/6827

    Abstract: The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample. The invention also provides samples, tissue slides, and kits relating to detection of nucleic acid variations, including single nucleotide variations, multi-nucleotide variations or splice sites, of a target nucleic acid.

    IN SITU DETECTION OF NUCLEOTIDE VARIANTS IN HIGH NOISE SAMPLES, AND COMPOSITIONS AND METHODS RELATED THERETO
    10.
    发明申请
    IN SITU DETECTION OF NUCLEOTIDE VARIANTS IN HIGH NOISE SAMPLES, AND COMPOSITIONS AND METHODS RELATED THERETO 审中-公开

    公开(公告)号：US20170101672A1

    公开(公告)日：2017-04-13

    申请号：US15291054

    申请日：2016-10-11

    Applicant: Advanced Cell Diagnostics, Inc.

    Inventor： Yuling Luo ,  Xingyong Wu ,  Liuliu Pan ,  Xiaoming Wang ,  Xiao-Jun Ma ,  Nan Su ,  Steve Chen

    IPC: C12Q1/68

    CPC classification number: C12Q1/6841 ,  C12Q1/682 ,  C12Q1/6827 ,  C12Q2565/102 ,  C12Q2525/313

    Abstract: The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample. The invention also provides samples, tissue slides, and kits relating to detection of nucleic acid variations, including single nucleotide variations, multi-nucleotide variations or splice sites, of a target nucleic acid.

Patent Agency Ranking