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    Guide RNAs with Chemical Modification for Prime Editing
    1.
    发明申请
    Guide RNAs with Chemical Modification for Prime Editing 有权

    公开(公告)号：US20230092393A1

    公开(公告)日：2023-03-23

    申请号：US17931535

    申请日：2022-09-12

    Applicant: Agilent Technologies, Inc.

    Inventor： Daniel E. Ryan ,  Robert Kaiser ,  Douglas J. Dellinger

    IPC: C12N15/10 ,  C12N9/22 ,  C12N15/62

    Abstract: Provided herein are compositions and methods for inducing CRISPR/Cas-based editing of a target nucleic acid (e.g., target DNA or target RNA) in vitro or in a cell, using modified prime editing guide RNAs (pegRNAs) that incorporate one or more chemically-modified nucleotides. The modified pegRNAs disclosed herein may be used to induce Cas-mediated incorporation of one or more nucleotide changes and/or targeted mutagenesis of a target nucleic acid. The nucleotide change can include, e.g., one or more nucleotide changes, an insertion of one or more nucleotides, or a deletion of one or more nucleotides.

    METHOD FOR PRODUCING A POPULATION OF OLIGONUCLEOTIDES THAT HAS REDUCED SYNTHESIS ERRORS
    3.
    发明申请
    METHOD FOR PRODUCING A POPULATION OF OLIGONUCLEOTIDES THAT HAS REDUCED SYNTHESIS ERRORS 审中-公开
    生产减少合成错误的寡核苷酸人群的方法

    公开(公告)号：US20150010953A1

    公开(公告)日：2015-01-08

    申请号：US13935201

    申请日：2013-07-03

    Applicant: AGILENT TECHNOLOGIES, INC.

    Inventor： Derek Lee Lindstrom ,  Jeffrey R. Sampson ,  Daniel E. Ryan

    IPC: C12N15/10 ,  C12P19/34

    CPC classification number: C12N15/1006 ,  C12N15/1003 ,  C12P19/34 ,  C12Q2521/514 ,  C12Q2525/301

    Abstract: Provided herein is a method for producing a population of oligonucleotides that has reduced synthesis errors. In certain embodiments, the method comprises: a) obtaining an initial population of hairpin oligonucleotide molecules that each comprise a double-stranded stem region and a loop region; b) contacting the double-stranded region of the hairpin oligonucleotide molecules with a mismatch binding protein; and c) eliminating any molecules that bind to the mismatch binding protein, thereby producing a population of oligonucleotides that has reduced synthesis errors. A kit and a composition for performing the method are also provided.

    Abstract translation: 本文提供了一种减少合成错误的寡核苷酸群体的制备方法。 在某些实施方案中，所述方法包括：a）获得每个包含双链茎区和环区的发夹寡核苷酸分子的初始群; b）使发夹寡核苷酸分子的双链区域与错配结合蛋白接触; 和c）消除与错配结合蛋白结合的任何分子，从而产生具有降低的合成错误的寡核苷酸群。 还提供了用于执行该方法的试剂盒和组合物。

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