公开(公告)号：US20180201924A1

公开(公告)日：2018-07-19

申请号：US15409124

申请日：2017-01-18

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  David Taussig ,  Bahram Arezi ,  Robert A. Ach ,  Nicholas M. Sampas

IPC: C12N15/10 ,  C12Q1/68

Abstract: A method for making an asymmetrically-tagged sequencing library is provided. In some embodiments, the method may comprise: obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, hybridizing a tailed first primer to the 3′ sequence tag of the library and extending the same to produce primer extension products, and amplifying the primer extension products using a prior of tailed primers to produce asymmetrically-tagged library.

公开(公告)号：US10711269B2

公开(公告)日：2020-07-14

申请号：US15409124

申请日：2017-01-18

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  David Taussig ,  Bahram Arezi ,  Robert A. Ach ,  Nicholas M. Sampas

IPC: C12N15/10 ,  C12Q1/6806

Abstract: A method for making an asymmetrically-tagged sequencing library is provided. In some embodiments, the method may comprise: obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, hybridizing a tailed first primer to the 3′ sequence tag of the library and extending the same to produce primer extension products, and amplifying the primer extension products using a pair of tailed primers to produce asymmetrically-tagged library.

公开(公告)号：US12281302B2

公开(公告)日：2025-04-22

申请号：US17879530

申请日：2022-08-02

Applicant: Agilent Technologies, Inc.

Inventor： David Taussig ,  Nicholas M. Sampas

IPC: C12N15/10

Abstract: The present disclosure relates to a method of assembling long nucleic acids by enzymatically ligating oligonucleotide molecules hybridized to an indexed splint oligonucleotide molecules. Also disclosed are oligonucleotide structures comprising an indexed splint oligonucleotide useful in performing the disclosed method.

公开(公告)号：US20240018510A1

公开(公告)日：2024-01-18

申请号：US18256877

申请日：2020-12-10

Applicant: Agilent Technologies, Inc.

Inventor： David Taussig ,  Israel Steinfeld ,  Nicholas M Sampas ,  Brian Jon Peter

IPC: C12N15/10 ,  C12Q1/6869

CPC classification number: C12N15/1065 ,  C12Q1/6869

Abstract: The present invention relates to preparation, sequencing and analysis of a sequencing library of adaptor-tagged fragments, wherein the fragments have different orientations relative to a sequencing adaptor.