公开(公告)号：US20170342483A1

公开(公告)日：2017-11-30

申请号：US15167823

申请日：2016-05-27

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Bahram Arezi ,  Michael Borns ,  Holly Hogrefe ,  Connie Hansen

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2521/507 ,  C12Q2525/179 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2565/514

Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.

公开(公告)号：US20180201924A1

公开(公告)日：2018-07-19

申请号：US15409124

申请日：2017-01-18

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  David Taussig ,  Bahram Arezi ,  Robert A. Ach ,  Nicholas M. Sampas

IPC: C12N15/10 ,  C12Q1/68

Abstract: A method for making an asymmetrically-tagged sequencing library is provided. In some embodiments, the method may comprise: obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, hybridizing a tailed first primer to the 3′ sequence tag of the library and extending the same to produce primer extension products, and amplifying the primer extension products using a prior of tailed primers to produce asymmetrically-tagged library.

公开(公告)号：USRE49207E1

公开(公告)日：2022-09-13

申请号：US16678868

申请日：2019-11-08

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Bahram Arezi ,  Michael Borns ,  Holly Hogrefe ,  Connie Hansen

IPC: C12P19/34 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.

公开(公告)号：US10240196B2

公开(公告)日：2019-03-26

申请号：US15167823

申请日：2016-05-27

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Bahram Arezi ,  Michael Borns ,  Holly Hogrefe ,  Connie Hansen

IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.

公开(公告)号：US10711269B2

公开(公告)日：2020-07-14

申请号：US15409124

申请日：2017-01-18

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  David Taussig ,  Bahram Arezi ,  Robert A. Ach ,  Nicholas M. Sampas

IPC: C12N15/10 ,  C12Q1/6806

Abstract: A method for making an asymmetrically-tagged sequencing library is provided. In some embodiments, the method may comprise: obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, hybridizing a tailed first primer to the 3′ sequence tag of the library and extending the same to produce primer extension products, and amplifying the primer extension products using a pair of tailed primers to produce asymmetrically-tagged library.

公开(公告)号：US09758773B2

公开(公告)日：2017-09-12

申请号：US14568549

申请日：2014-12-12

Applicant: Agilent Technologies, Inc.

Inventor： Nancy Mckinney ,  Holly H. Hogrefe ,  Jeffrey Fox ,  Connie J. Hansen ,  Bahram Arezi

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

Abstract: The invention provides mutants of DNA polymerases having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases.

公开(公告)号：US20160340657A1

公开(公告)日：2016-11-24

申请号：US15089749

申请日：2016-04-04

Applicant: Agilent Technologies, Inc.

Inventor： Holly H. Hogrefe ,  Bahram Arezi ,  Weimei Xing

IPC: C12N9/12 ,  C12Q1/68 ,  C12N15/10

CPC classification number: C12N9/1276 ,  C12N15/1096 ,  C12Q1/686 ,  C12Y207/07049 ,  C40B40/08 ,  C40B50/06

Abstract: The invention relates to the generation and characterization of stable MMLV reverse transcriptase mutants. The invention also discloses methods of using stable MMLV reverse transcriptase mutants.

Abstract translation: 本发明涉及稳定的MMLV逆转录酶突变体的产生和表征。 本发明还公开了使用稳定的MMLV逆转录酶突变体的方法。

公开(公告)号：US20150232821A1

公开(公告)日：2015-08-20

申请号：US14568549

申请日：2014-12-12

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Nancy Mckinney ,  Holly H. Hogrefe ,  Jeffrey Fox ,  Connie J. Hansen ,  Bahram Arezi

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

Abstract: The invention provides mutants of DNA polymerases having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases.

Abstract translation: 本发明提供对DNA聚合酶活性抑制剂具有增强抗性的DNA聚合酶突变体。 突变型聚合酶非常适用于含有野生型聚合酶抑制剂的样品中靶标的PCR扩增。