公开(公告)号：US11988633B2

公开(公告)日：2024-05-21

申请号：US16644467

申请日：2018-09-20

Applicant: Agilent Technologies, Inc.

Inventor： Fielito U. Limjap ,  Michael J. Kimzey ,  Francis T. Haxo

IPC: G01N27/447 ,  C08B37/00 ,  C08G73/02 ,  C08L79/02

CPC classification number: G01N27/44747 ,  C08B37/0003 ,  C08B37/0087 ,  C08G73/0206 ,  C08G73/0273 ,  C08L79/02 ,  G01N27/44791

Abstract: The present disclosure provides methods of improving the resolution of labeled glycans in capillary electrophoresis techniques using a gel as a sieving matrix, by incorporating polyamines in the gel.

公开(公告)号：US20210223253A1

公开(公告)日：2021-07-22

申请号：US17222741

申请日：2021-04-05

Applicant: Agilent Technologies, Inc.

Inventor： Michael J. Kimzey ,  Alexander Gyenes

IPC: G01N33/58 ,  C07D207/46

Abstract: The invention relates to an activated form of procaine, and the use of the activated procaine, or salts or solvates thereof, to label amine-containing compounds. In some embodiments, the amine-containing compound is an N-glycan.

公开(公告)号：US20210333269A1

公开(公告)日：2021-10-28

申请号：US17369768

申请日：2021-07-07

Applicant: Agilent Technologies, Inc.

Inventor： Francis T. Haxo ,  Michael J. Kimzey

IPC: G01N33/533 ,  C07D207/46 ,  C07D213/24 ,  C12Q1/6806 ,  C07H1/00

Abstract: Bispyridines improve the labeling of nucleophiles, including amines and thiols and are particularly useful for improving labeling with acidic and basic labels. Use of bispyridines with such labels dramatically increases labeling compared to protocols without a bispyridine. The labeled nucleophile can then be subjected to standard analytical methods.

公开(公告)号：US11815488B2

公开(公告)日：2023-11-14

申请号：US16960307

申请日：2019-01-11

Applicant: Agilent Technologies, Inc.

Inventor： Michael J. Kimzey ,  Fielito U. Limjap ,  Francis T. Haxo ,  Sergey Vlasenko

IPC: G01N27/447 ,  C12N15/10

CPC classification number: G01N27/44747 ,  C12N15/101 ,  G01N27/44791

Abstract: The present disclosure provides methods of improving the resolution of analytes by in electrophoretic separations using a gel by incorporating into the gel an effective amount of one or more tri- or tetra-hydroxyl quaternary ammonium compounds, or a mixture of such compounds.

公开(公告)号：US11092595B2

公开(公告)日：2021-08-17

申请号：US16072816

申请日：2017-02-24

Applicant: Agilent Technologies, Inc.

Inventor： Francis T. Haxo ,  Michael J. Kimzey

IPC: G01N33/533 ,  C07D207/46 ,  C07D213/24 ,  C12Q1/6806 ,  C07H1/00

Abstract: Bispyridines improve the labeling of nucleophiles, including amines and thiols and are particularly useful for improving labeling with acidic and basic labels. Use of bispyridines with such labels dramatically increases labeling compared to protocols without a bispyridine. The labeled nucleophile can then be subjected to standard analytical methods.

公开(公告)号：US11858961B2

公开(公告)日：2024-01-02

申请号：US17398763

申请日：2021-08-10

Applicant: Agilent Technologies, Inc.

Inventor： Michael J. Kimzey ,  Francis T. Haxo ,  Vaishali Sharma

IPC: C07K1/00 ,  C07K1/113 ,  C11D1/62 ,  C11D17/00

CPC classification number: C07K1/1136 ,  C07K1/113 ,  C11D1/62 ,  C11D17/00

Abstract: Ammonium cation detergents comprising a quaternary or tertiary ammonium cation can be used as detergents to denature proteins and are particularly useful in denaturing glycoproteins or glycopeptides prior to enzymatic deglycosylation. Ammonium cation detergents with sulfate or sulfonate anions are particularly useful.

公开(公告)号：US20210263043A1

公开(公告)日：2021-08-26

申请号：US17319868

申请日：2021-05-13

Applicant: Agilent Technologies, Inc.

Inventor： Michael J. Kimzey

IPC: G01N33/68 ,  G01N33/53 ,  C09B19/00 ,  C09B57/02 ,  C09B57/08 ,  C12P19/04 ,  G01N33/58

Abstract: The presence of mild electrophiles, such as aldehydes, during the denaturation of glycoproteins or glycopeptides and subsequent enzymatic deglycosylation reduces artifacts in subsequent analyses of the glycans released from the glycoproteins or gly-copeptides.