公开(公告)号：US20230024869A1

公开(公告)日：2023-01-26

申请号：US17933003

申请日：2022-09-16

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Leendert W. NEUTEBOOM ,  John A. McElver ,  Rosa De Pinho Barroco ,  Chris De Wilde ,  Max Fabian Felle

IPC: C12N15/11 ,  C12N15/70 ,  C12N15/75 ,  C12N15/81 ,  C12N15/82 ,  C12N15/85 ,  C12N15/90 ,  C12N15/10

Abstract: Methods for modification of target nucleic acids. The method involves a construct in which guide RNA is covalently linked to donor RNA (fusion NA) to be introduced into the target nucleic acid by homologous recombination and is based on the introduction of a nuclease, e.g. CRISPR or TALEN, into the cell containing the target nucleic acid. The fusion NA may be introduced as a DNA vector.

公开(公告)号：US20230049124A1

公开(公告)日：2023-02-16

申请号：US17933015

申请日：2022-09-16

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Leendert W. NEUTEBOOM ,  John A. Mcelver ,  Rosa De Pinho Barroco ,  Chris De Wilde ,  Max Fabian Felle

IPC: C12N15/11 ,  C12N15/70 ,  C12N15/75 ,  C12N15/81 ,  C12N15/82 ,  C12N15/85 ,  C12N15/90 ,  C12N15/10

Abstract: Methods for modification of target nucleic acids. The method involves a construct in which guide RNA is covalently linked to donor RNA (fusion NA) to be introduced into the target nucleic acid by homologous recombination and is based on the introduction of a nuclease, e.g. CRISPR or TALEN, into the cell containing the target nucleic acid. The fusion NA may be introduced as a DNA vector.