公开(公告)号：US20150317458A1

公开(公告)日：2015-11-05

申请号：US14650059

申请日：2013-12-05

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Katrin Lotz ,  Michael Leps ,  Rainer Lemke ,  Bjoern Junker ,  Falk Schreiber

IPC: G06F19/00 ,  C12N15/82

CPC classification number: G16C10/00 ,  A01H1/00 ,  C12N15/8216 ,  C12N15/8243 ,  C12N15/8254

Abstract: The present invention relates to a method for identifying at least one metabolic conversion step, the modulation of which increases the amount of a metabolite of interest in a plant cell, plant or plant part, said method comprising establishing a stoichiometric network model for the metabolism of the plant cell, plant or plant part including the synthesis pathway for the metabolite of interest, identifying at least one candidate metabolic conversion step by applying at least one algorithm of Growth-coupled Design, and validating the at least one candidate metabolic conversion step by a constraint-based modeling approach in the stoichiometric network model, wherein an increase in the metabolite of interest occurring in said constraint-based modeling approach is indicative for a metabolic conversion step, the modulation of which increases the amount of the metabolite of interest in the plant cell, plant or plant part. The present invention further relates to a method for generating a plant cell, plant or plant part which produces an increased amount of a metabolite of interest when compared to a control, said method comprising identifying a metabolic conversion step, the modulation of which increases a metabolite of interest in a plant cell, plant or plant part, by the method for identifying a metabolic conversion step and modulating the said metabolic conversion step such that the amount of the metabolite of interest is increased in vivo in a plant cell, plant or plant part.

Abstract translation: 本发明涉及用于鉴定至少一种代谢转化步骤的方法，所述代谢转化步骤的调节增加植物细胞，植物或植物部分中感兴趣的代谢物的量，所述方法包括建立用于代谢的化学计量网络模型 所述植物细胞，植物或植物部分包括所述感兴趣代谢物的合成途径，通过应用至少一种生长耦合设计算法来鉴定至少一种候选代谢转化步骤，以及通过以下步骤验证所述至少一种候选代谢转化步骤： 在化学计量网络模型中基于约束的建模方法，其中在所述基于约束的建模方法中发生的感兴趣代谢物的增加指示代谢转化步骤，其调节增加植物中感兴趣的代谢物的量 细胞，植物或植物部分。 本发明还涉及当与对照相比时产生植物细胞，植物或植物部分的方法，其产生增加的感兴趣代谢物的量，所述方法包括鉴定代谢转化步骤，其调节增加代谢物 感兴趣的植物细胞，植物或植物部分，通过鉴定代谢转化步骤的方法和调节所述代谢转化步骤，使得感兴趣的代谢物的量在植物细胞，植物或植物部分的体内增加 。