公开(公告)号：US11142545B2

公开(公告)日：2021-10-12

申请号：US16312819

申请日：2017-06-22

Applicant: BIOGEN MA INC.

Inventor： Xianglin Shi ,  Jimin Yang ,  William F. Kiesman ,  Yannick Fillon

IPC: C07H21/04 ,  C07H1/00 ,  C07H21/02 ,  C07H21/00

Abstract: The invention herein describes a solid-phase synthetic method for preparing thiolated oligonucleotides without needing a capping step. The methods of the invention comprises repetition of a three-reaction per cycle, namely detritylation, coupling and sulfurization, without a capping step. In some embodiments, the synthetic methods of the present invention can be used for preparing an anti-sense oligonucleotide.

公开(公告)号：US20190161513A1

公开(公告)日：2019-05-30

申请号：US16312819

申请日：2017-06-22

Applicant: BIOGEN MA INC.

Inventor： Xianglin Shi ,  Jimin Yang ,  William F. Kiesman ,  Yannick Fillon

IPC: C07H21/04 ,  C07H21/02 ,  C07H1/00

Abstract: The invention herein describes a synthetic method for preparing thiolated oligonucleotides without needing a capping step, as the sulfurization agent caps the unreacted 5′-OH groups.