公开(公告)号：US10947262B2

公开(公告)日：2021-03-16

申请号：US16309622

申请日：2017-06-13

Applicant: BIOGEN MA INC.

Inventor： Robert S. Gronke ,  Ratnesh S. Joshi

IPC: C07H1/06 ,  C07H1/00 ,  B01D15/30 ,  B01D15/42 ,  C07H21/02 ,  C07H21/04

Abstract: The invention herein describes a method to purify a target oligonucleotide using hydrophobic interaction chromatography (HIC). The method includes adding a salt to a mixture of the target oligonucleotide and product-related impurities, applying the diluted mixture, at a particular dynamic loading capacity, to the hydrophobic interaction chromatography resin (or hydrophobic adsorbent), washing the hydrophobic adsorbent with an aqueous salt solution, eluting the target oligonucleotide with a eluting solution, and collecting the eluent comprising the target oligonucleotide.

公开(公告)号：US20250011357A1

公开(公告)日：2025-01-09

申请号：US18742011

申请日：2024-06-13

Applicant: Biogen MA Inc.

Inventor： Robert S. Gronke ,  Ratnesh S. Joshi

IPC: C07H1/06 ,  B01D15/30 ,  B01D15/42 ,  C07H1/00 ,  C07H21/02 ,  C07H21/04

Abstract: The present invention is directed to a method of purifying oligonucleotides using hydrophobic interaction chromatography.

公开(公告)号：US20210269467A1

公开(公告)日：2021-09-02

申请号：US17199921

申请日：2021-03-12

Applicant: BIOGEN MA INC.

Inventor： Robert S. Gronke ,  Ratnesh S. Joshi

IPC: C07H1/06 ,  C07H1/00 ,  B01D15/30 ,  B01D15/42 ,  C07H21/02 ,  C07H21/04

Abstract: The present invention is directed to a method of purifying oligonucleotides using hydrophobic interaction chromatography.

公开(公告)号：US20190248823A1

公开(公告)日：2019-08-15

申请号：US16309622

申请日：2017-06-13

Applicant: BIOGEN MA INC.

Inventor： Robert S. Gronke ,  Ratnesh S. Joshi

IPC: C07H1/06 ,  C07H21/04 ,  C07H21/02 ,  B01D15/30 ,  B01D15/42

CPC classification number: C07H1/06 ,  B01D15/305 ,  B01D15/426 ,  C07H1/00 ,  C07H21/02 ,  C07H21/04

Abstract: The present invention is directed to a method of purifying oligonucleotides hydrophobic interaction chromatography.