公开(公告)号：US3910819A

公开(公告)日：1975-10-07

申请号：US44375174

申请日：1974-02-19

Applicant: CALIFORNIA INST OF TECHN

Inventor： REMBAUM ALAN ,  INGRAM MARYLOU ,  STROUD-SCHMINK F AGNES ,  ROUNDS DONALD E

IPC: C12N1/38 ,  C12N5/00 ,  C12B3/00

CPC classification number: C12N5/0068 ,  C12N1/38 ,  C12N2533/50

Abstract: Contact of negatively charged biological cells is promoted by dispersion within the cell medium of a minor amount of a polyquaternary polymeric amine known as an ionene. Adhesion of the cells to normally negatively charged surfaces such as glass or plastic tissue culture devices is stimulated by pretreating the surfaces of the devices with a dilute solution of the ionene polymer. Increased cell adhesion and growth is demonstrated for both normal and transformed cells. Experiments have verified that cells grow at a more rapid rate on the surface of an ionene treated container than in the most effective treated containers that are commercially available.

Abstract translation: 带负电荷的生物细胞的接触通过在少量称为紫罗烯的多季铵聚合胺的细胞培养基内的分散来促进。 通过用紫罗烯聚合物的稀溶液预处理器件的表面来刺激细胞对通常带负电的表面如玻璃或塑料组织培养装置的粘附。 对正常细胞和转化细胞都证实增加细胞粘附和生长。 实验已经证实，细胞在经紫外线处理的容器的表面上比在市售的最有效的处理容器中以更快的速率生长。