公开(公告)号：US20240132538A1

公开(公告)日：2024-04-25

申请号：US17768461

申请日：2020-11-20

Applicant: Cytiva Bioprocess R&D AB

Inventor： Christopher James Sevinsky ,  Peter Lundback ,  Johan Ohman ,  Gregory Grossmann ,  Sean R. Dinn

IPC: C07K1/22 ,  C07K14/195 ,  C07K17/10

CPC classification number: C07K1/22 ,  C07K14/195 ,  C07K17/10 ,  C07K2319/92

Abstract: The present invention relates to protein purification, primarily in the chromatographic field. More closely, the invention relates to affinity chromatography using a split intein system with an improved C-intein tag and N-intein ligand, wherein the target protein may be purified as a tag-less end product with a native N-terminus.

公开(公告)号：US20240076650A1

公开(公告)日：2024-03-07

申请号：US18260560

申请日：2022-01-26

Applicant: CYTIVA BIOPROCESS R&D AB

Inventor： Peter Lundback ,  Ronnie Palmgren ,  Ola Lind ,  Nils Norrman ,  Helen Cheek ,  Susana Brito Dos Santos ,  Per Denker

IPC: C12N15/10 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/289

CPC classification number: C12N15/101 ,  B01D15/3819 ,  B01J20/265 ,  B01J20/28035 ,  B01J20/28038 ,  B01J20/289 ,  B01J2220/52

Abstract: The invention relates to processes for purification of poly-tagged products, such as mRNA, from synthetic or biological compositions. The process involves contacting the composition with a oligo d (T)-functionalized chromatography medium comprising a convection-based chromatography material.