公开(公告)号：US20140087359A1

公开(公告)日：2014-03-27

申请号：US14033365

申请日：2013-09-20

Applicant: California Institute of Technology

Inventor： Samuel NJOROGE ,  John GORMAN ,  George MALTEZOS ,  Axel SCHERER

IPC: C12M1/00 ,  C12N1/06

CPC classification number: C12M47/06 ,  B01L3/5027 ,  B01L7/52 ,  B01L2300/1883 ,  C12M21/00 ,  C12N1/06 ,  C12N1/066 ,  G01N27/44791

Abstract: Disclosed herein are methods and systems for use in preparing a sample. The methods and systems may be used for lysing one or more structures in a sample (e.g., cells, viral particles, etc.). The methods and compositions may comprise a microfluidic chip or use thereof. The microfluidic chips disclosed herein may comprise (a) a substrate comprising a chamber, wherein at least one mechanical element may be located within the chamber; (b) a thermal element in contact with the chamber; and (c) at least one aperture within the surface of the substrate, wherein the aperture may be configured to insulate the chamber.

Abstract translation: 本文公开了用于制备样品的方法和系统。 方法和系统可用于裂解样品中的一种或多种结构（例如，细胞，病毒颗粒等）。 所述方法和组合物可以包含微流控芯片或其用途。 本文公开的微流体芯片可以包括（a）包括腔室的基底，其中至少一个机械元件可以位于腔室内; （b）与所述室接触的热元件; 以及（c）在所述基底表面内的至少一个孔，其中所述孔可以被配置为使所述腔绝缘。