公开(公告)号：US20150209690A1

公开(公告)日：2015-07-30

申请号：US14614376

申请日：2015-02-04

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： George MALTEZOS ,  Alvaro GOMEZ ,  Frank GOMEZ ,  Axel SCHERER

IPC: B01D15/22 ,  B29C65/70 ,  B01L3/00

CPC classification number: B01D15/22 ,  B01L3/502707 ,  B01L3/502753 ,  B01L2300/12 ,  B01L2300/16 ,  B29C65/70 ,  B29K2027/12 ,  B29L2031/756 ,  G01N30/603 ,  G01N30/6095 ,  Y10T29/49826

Abstract: A device and method for making a microfluidic separation device. A microfluidic separation device could include a microfluidic column having an inlet, the microfluidic column being configured to hold a first fluid and the microfluidic column including a porous portion, and an outlet attached to the microfluidic column, the outlet being configured to output a second fluid. The method may include providing a microfluidic column having an inlet, configuring the microfluidic column to hold a first fluid, forming a porous portion in the microfluidic column, and attaching an outlet to the microfluidic column.

Abstract translation: 用于制造微流体分离装置的装置和方法。 微流体分离装置可以包括具有入口的微流体柱，所述微流体柱被配置为保持第一流体，并且所述微流体柱包括多孔部分和连接到所述微流体柱的出口，所述出口构造成输出第二流体 。 该方法可以包括提供具有入口的微流体柱，构造微流体柱以保持第一流体，在微流体柱中形成多孔部分，以及将出口附接到微流体柱。

公开(公告)号：US20140087359A1

公开(公告)日：2014-03-27

申请号：US14033365

申请日：2013-09-20

Applicant: California Institute of Technology

Inventor： Samuel NJOROGE ,  John GORMAN ,  George MALTEZOS ,  Axel SCHERER

IPC: C12M1/00 ,  C12N1/06

CPC classification number: C12M47/06 ,  B01L3/5027 ,  B01L7/52 ,  B01L2300/1883 ,  C12M21/00 ,  C12N1/06 ,  C12N1/066 ,  G01N27/44791

Abstract: Disclosed herein are methods and systems for use in preparing a sample. The methods and systems may be used for lysing one or more structures in a sample (e.g., cells, viral particles, etc.). The methods and compositions may comprise a microfluidic chip or use thereof. The microfluidic chips disclosed herein may comprise (a) a substrate comprising a chamber, wherein at least one mechanical element may be located within the chamber; (b) a thermal element in contact with the chamber; and (c) at least one aperture within the surface of the substrate, wherein the aperture may be configured to insulate the chamber.

Abstract translation: 本文公开了用于制备样品的方法和系统。 方法和系统可用于裂解样品中的一种或多种结构（例如，细胞，病毒颗粒等）。 所述方法和组合物可以包含微流控芯片或其用途。 本文公开的微流体芯片可以包括（a）包括腔室的基底，其中至少一个机械元件可以位于腔室内; （b）与所述室接触的热元件; 以及（c）在所述基底表面内的至少一个孔，其中所述孔可以被配置为使所述腔绝缘。