公开(公告)号：US12180507B2

公开(公告)日：2024-12-31

申请号：US15749733

申请日：2016-08-05

Applicant: Colorado State University Research Foundation

Inventor： Salman R. Khetani ,  Mitchell J. Durham ,  Brenton R. Ware

IPC: C12N5/00 ,  A61K35/407 ,  A61K35/44 ,  C12N5/071 ,  G01N33/50

Abstract: The disclosure relates to in vitro cultures of human hepatocytes, and in particular co-cultures systems including human hepatocytes, non-parenchymal cells, and human endothelial cells, and the use of the co-cultures in developing and screening drugs.

公开(公告)号：US10717966B2

公开(公告)日：2020-07-21

申请号：US16157930

申请日：2018-10-11

Applicant: Colorado State University Research Foundation

Inventor： Salman R. Khetani ,  Dustin R. Berger ,  Brenton R. Ware

IPC: C12N5/071

Abstract: The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

公开(公告)号：US20190048315A1

公开(公告)日：2019-02-14

申请号：US16157930

申请日：2018-10-11

Applicant: Colorado State University Research Foundation

Inventor： Salman R. Khetani ,  Dustin R. Berger ,  Brenton R. Ware

IPC: C12N5/071

Abstract: The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

公开(公告)号：US20180223253A1

公开(公告)日：2018-08-09

申请号：US15749733

申请日：2016-08-05

Applicant: Colorado State University Research Foundation

Inventor： Salman R. Khetani ,  Mitchell J. Durham ,  Brenton R. Ware

IPC: C12N5/071 ,  A61K35/407 ,  A61K35/44 ,  G01N33/50

Abstract: The disclosure relates to in vitro cultures of human hepatocytes, and in particular co-cultures systems including human hepatocytes, non-parenchymal cells, and human endothelial cells, and the use of the co-cultures in developing and screening drugs.

公开(公告)号：US10266806B2

公开(公告)日：2019-04-23

申请号：US14596787

申请日：2015-01-14

Applicant: Colorado State University Research Foundation

Inventor： Salman R. Khetani ,  Dustin R. Berger ,  Brenton R. Ware

IPC: C12N5/07 ,  C12N5/071

Abstract: The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

公开(公告)号：US20150240203A1

公开(公告)日：2015-08-27

申请号：US14596787

申请日：2015-01-14

Applicant: Colorado State University Research Foundation

Inventor： Salman R. Khetani ,  Dustin R. Berger ,  Brenton R. Ware

IPC: C12N5/071 ,  G01N33/50

CPC classification number: C12N5/067 ,  C12N2501/237 ,  C12N2501/39 ,  C12N2502/13 ,  C12N2503/02 ,  C12N2533/90

Abstract: The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

Abstract translation: 本公开提供了体外人多能干细胞衍生的肝细胞和至少一种非实质细胞群体的共培养物，制备共培养物的方法和使用共培养物进行高通量筛选和评估候选药物的方法 。 干细胞衍生的肝细胞共培养系统提供了体外模型，其中相对于常规单一培养，干细胞来源的肝细胞的细胞存活力和相对成熟的肝细胞表型保持较长的时间。