公开(公告)号：US11285460B2

公开(公告)日：2022-03-29

申请号：US15120845

申请日：2015-02-18

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Karolina Busson ,  Karol Lacki ,  Bjorn Noren ,  Helena Skoglar

IPC: B01D15/12 ,  B01D15/20 ,  B01D15/30 ,  B01D15/36 ,  B01J20/28 ,  B01J20/286 ,  B01J20/32 ,  C07K1/22 ,  C07K14/765 ,  C07K14/77 ,  C12N1/20

Abstract: The invention discloses a separation matrix for purification of biological particles, comprising a plurality of particles having a porous core entity and a porous shell entity covering the core entity, wherein the core entity comprises at least 50 micromole/ml primary amines present on covalently attached ligands displaying at least two primary amines per ligand and the shell entity comprises less than 20 micromole/ml primary amines The invention further discloses a method of purifying biological particles and a method of manufacturing a separation matrix.