NUCLEIC ACIDS EXTRACTION SYSTEM AND METHOD BASED ON 3D-PRINTED MICRODEVICE

    公开(公告)号:US20230146223A1

    公开(公告)日:2023-05-11

    申请号:US17914974

    申请日:2021-06-18

    CPC classification number: C12N15/1006 B33Y80/00 B29C64/124

    Abstract: The present invention discloses a nucleic acids extraction system and method based on a 3D-printed microdevice, and belongs to the technical field of nucleic acids extraction. The nucleic acids extraction system is a monomer 3D-printed microdevice or a 3D-printed microdevice prepared by 3D printing technologies, the monomer 3D-printed microdevice comprises a nucleic acids binding region and a handle region, and the 3D-printed microdevice is composed of more than two monomer 3D-printed microdevices through a joining region; and the nucleic acids binding region is made of photosensitive resin or thermoplastic. An extraction method for the nucleic acids extraction system based on a 3D-printed microdevice is used to bind, clean and elute nucleic acids by moving the monomer 3D-printed microdevice or the 3D-printed microdevice among a solution containing target nucleic acids, a washing buffer and an elution buffer.

    CONTINUOUS CRYSTALLIZATION METHOD UNDER CONTROL OF MULTISTAGE MEMBRANE

    公开(公告)号:US20200114277A1

    公开(公告)日:2020-04-16

    申请号:US16314773

    申请日:2017-11-03

    Abstract: The present invention provides a continuous crystallization method under control of the multistage membrane modules, and belongs to the technical field of crystallization engineering. A crystallization solution is added to a crystallizer, and a stirring apparatus and a temperature control apparatus are started. After the system running stability, the loop of crystallization is started. Meanwhile, the coolant or antisolvent feed liquid loop is also started. The crystallization solution can respectively conduct crystal nucleation, growth and ripening in the multistage membrane modules, and then the crystallization solution is transported into a filter device and a drying apparatus to obtain the final crystal products. The desired crystal products can be obtained by the systematical control of the nucleation and crystal growth through the flow and the temperature of the crystallization solution, coolant or antisolvent feed liquid, and the contact time between two liquid phases.

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